Determination of 4-dimethylaminophenol concentrations in dog blood using LC–ESI/MS/MS combined with precolumn derivatization

Enhancement of the capabilities of liquid chromatography–mass spectrometry with derivatization: General principles and applications

“…Phenol group containing drugs, such as propofol (Beaudry et al, 2005), icariin (Gong et al, 2007), eugenol (Guenette et al, 2006), ethinyl estradiol (Li et al, 2005a), 17 b-estradiol-d 4 (E2-d 4 ), and its metabolite estrone-d 4 (E1-d 4 ) (Xia et al, 2004), and 4-dimethylaminophenol (DMAP) (Zhuang et al, 2008) all showed a poor MS response. Thus, DNS-Cl derivatization was used in combination with LC-MS for sensitive and selective determinations of these drugs in a biological matrix through the introduction of an easily ionizable tertiary amine (>N-).…”

Derivatization reagents in liquid chromatography/electrospray ionization tandem mass spectrometry

“…SRM using the transition of quasi‐molecular ion of the derivative to m/z 171 or 170 resulted in a sensitive detection of the derivatives. So far, it was used for determination of steroids having hydroxyl group such as estradiol, ethinyl estradiol, estrone and the related compounds (Anari et al ., 2002; Xia et al ., 2004; Shou et al ., 2004; Zhang et al ., 2004; Nelson et al ., 2004; Xu et al ., 2005; Tai and Welch, 2005; Li et al ., 2005a; Lin et al ., 2007; Pedreira et al ., 2007; Kushnir et al ., 2008; Farlow et al ., 2009; Petucci et al ., 2010; Scarth et al ., 2010), propofol (veterinary medicine; Beaudry et al ., 2005), 1‐hydroxypyrene (biomarker to monitor exposure to polycyclic aromatic hydrocarbons; Li et al ., 2005b), icariin (Gong et al ., 2007), 4‐dimethylaminophenol (Zhuang et al ., 2008) and multiple classes of phenols in blood (Chang et al ., 2010).…”

Derivatization in liquid chromatography for mass spectrometric detection

“…The generated derivatives were sensitively analyzed by LC/ESI-MS/MS (11). DnsCl is widely used for the analysis of the compounds having hydroxyl group (10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Similarly, picolinic acid (20-26), fusaric acid (27), dimethylglycine (28), isonicotinoyl azide (29,30), and N-methyl-nicotinic acid N-hydroxysuccinimide ester (C1-NA-NHS) (31) were used for the derivatization of alcohols and phenols.…”