Determination of 5‐Aminosalicylic Acid by Catalase‐Peroxidase Based Biosensor

Akkaya, Alper; Altuğ, Çağrı; Pazarlıoǧlu, Nurdan Kaşıkara; Dinçkaya, Erhan

doi:10.1002/elan.200904606

Cited by 18 publications

(12 citation statements)

References 23 publications

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“…In comparison, electrochemical sensors and biosensors have some advantages such as high sensitivity, inexpensive equipments, simple modification of working electrodes and they can be used in “in vivo” and “in situ ” biological studies. To our knowledge, in the recent years limited number of studies can be found in literature on electrochemical determinations of MSA 24–28.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Electrochemical Behavior of Mesalazine on the Surface of a Glassy Carbon Electrode Modified with CNT/PPY Doped by 1,5‐Naphthalenedisulfonic Acid

2013

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A glassy carbon electrode (GCE) was modified with a thin layer of multiwalled carbon nanotubes (MWCNTs) and subsequently, electrochemically deposited poly‐pyrrole. The electrochemical behavior of mesalazine was studied on the surface of the modified electrode by applying linear sweep voltammetry (LSV). The electropolymerization process and the electrochemical response toward mesalazine were investigated in the presence of different aromatic anion dopants including, benzenesulfonic acid (BSA), 1,3‐benzenedisulfonic acid (1,3‐BDSA), 1,5‐naphthalenedisulfonic acid (1,5‐NDSA) and new coccine (NC). By using 1,5‐NDSA as dopant, a significant increase (∼418 times) in the peak current of mesalazine was observed, in comparison to the bare GCE. Experimental variables such as drop size of the cast MWCNTs suspension, pH of the supporting electrolyte, accumulation conditions and the number of scans in the electropolymerization process were optimized by monitoring the LSV responses of mesalazine. Under the optimum conditions, two linear dynamic ranges of 0.01–0.1 µmol L−1 and 0.1–1.0 µmol L−1 with a detection limit of 3 nmol L−1 were resulted for the voltammetric determination of mesalazine. The prepared electrode showed high sensitivity, stability and good reproducibility for determination of mesalazine. These properties made the prepared sensor suitable for the determination of mesalazine in pharmaceutical and clinical preparations.

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Section: Introductionmentioning

confidence: 99%

Investigation of the Electrochemical Behavior of Mesalazine on the Surface of a Glassy Carbon Electrode Modified with CNT/PPY Doped by 1,5‐Naphthalenedisulfonic Acid

2013

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“…Catalase and horseradish peroxidase were co-entrapped in a gelatin matrix to prepare a biosensor on the surface of a Teflon thin membrane covering a gold electrode for the determination of 5-aminosalicylic acid. The biosensor retained 91 % of its activity after 21 days of storage at 4 C in a wet atmosphere [30].…”

Section: Immobilization Techniquesmentioning

confidence: 99%

Electrochemical Biosensors for Drug Analysis

Özkan

Kauffmann

Zuman

2015

Monographs in Electrochemistry

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“…In 2011, electrochemical sensors for the paracetamol were also reported. In 2009, Akkaya et al [143] reported a catalase-peroxidase-based biosensor for the determination of 5-aminosalicylic acid (5-ASA, mesalazine), which is an antiinflammatory drug used to treat inflammation of the digestive tract (Crohn's disease). The LOD obtained was 2.5 nM in buffer.…”

Section: Analgesics and Anti-inflammatory Compoundsmentioning

confidence: 99%