Determination of 60 pesticides in hen eggs using the QuEChERS procedure followed by LC-MS/MS and GC-MS/MS

Song, Nho‐Eul; Lee, Jun Young; Mansur, Ahmad Rois; Jang, Hae Won; Lim, Min-Cheol; Lee, Yunyeol; Yoo, Miyoung; Nam, Tae Gyu

doi:10.1016/j.foodchem.2019.125050

Cited by 109 publications

(49 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The original QuEChERS method introduced by Anastassiades, Lehotay, Štajnbaher, and Schenck (2003) has been modified to be more suitable for the analysis of some pesticides, including those with planar structures, different polarities, and pHdependent characteristics. The dispersive solid-phase extraction (d-SPE) technique was developed simultaneously with the QuEChERS method to minimize interference (Song et al, 2019). In d-SPE, a primary secondary amine (PSA) sorbent removes polar interferents (organic acids and sugars), while octadecylsilane (C 18 ) is used to remove non-polar co-eluents such as lipids, and graphitized carbon black (GCB) is used to remove pigmented matrices such as chlorophyll and carotenoids.…”

Section: Introductionmentioning

confidence: 99%

Multi-residue analysis of 203 pesticides in strawberries by liquid chromatography tandem mass spectrometry in combination with the QuEChERS method

Song

Yoo

Nam

2019

CyTA - Journal of Food

Self Cite

View full text Add to dashboard Cite

Herein, a QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure together with liquid chromatography and tandem mass spectrometry was applied to the multi-residue analysis of 203 pesticides in strawberries. To reduce the amount of co-eluents, we compared several dispersive solidphase extraction cleanup procedures and found that cleanup using octadecylsilane provided the best recoveries. Spiked-sample recoveries of 70-120% with relative standard deviations of <20% were obtained for all tested pesticides except for quinmerac. Matrix-matched calibration curves exhibited good linearities (R 2 > 0.99), and the limits of quantification (LOQs) were lower than the maximum residue limits recommended by the European Commission and Korea Food and Drug Administration. The developed method was applied to real samples, revealing that their pesticide contents exceeded the corresponding LOQs. The obtained results demonstrate that octadecylsilane-based dispersive solid-phase extraction is a rapid and high-throughput cleanup procedure for the multi-residue analysis of pesticides in strawberries. Análisis de residuos múltiples de 203 pesticidas detectados en fresas empleando cromatografía líquida de espectrometría de masas en tándem en combinación con el método QuEChERS RESUMEN El presente estudio aplicó un procedimiento QuEChERS [acrónimo en inglés de rápido, fácil, barato, efectivo, resistente y seguro] junto con cromatografía líquida y espectrometría de masas en tándem para analizar residuos múltiples de 203 pesticidas detectados en fresas. Con el propósito de reducir la cantidad de coeluyentes, se compararon varios procedimientos de limpieza de extracción en fase sólida dispersiva, constatándose que la limpieza con octadecilsilano proporcionó las mejores recuperaciones. Así, se obtuvieron recuperaciones de muestras con picos de 70-120% y desviaciones estándar relativas <20% para todos los pesticidas analizados, excepto quinmerac. Las curvas de calibración de matriz coincidente exhibieron buenas linealidades (R 2 > 0.99), mientras que los límites de cuantificación (LOQ) fueron inferiores a los límites máximos de residuos (LMR) recomendados por la Comisión Europea y la Administración de Alimentos y Medicamentos de Corea. El método desarrollado se aplicó a muestras reales, revelando que los contenidos de pesticidas de éstas excedían los LOQ correspondientes. Los resultados obtenidos demuestran que la extracción dispersiva en fase sólida basada en octadecilsilano es un procedimiento de limpieza rápido y de alto rendimiento para analizar residuos múltiples de pesticidas en fresas.

show abstract

Section: Introductionmentioning

confidence: 99%

Multi-residue analysis of 203 pesticides in strawberries by liquid chromatography tandem mass spectrometry in combination with the QuEChERS method

Song

Yoo

Nam

2019

CyTA - Journal of Food

Self Cite

View full text Add to dashboard Cite

show abstract

“…Heavy metal contamination, presence of potential hepatotoxic volatile organic compounds, adulterants, and insecticides and pesticides were analyzed in the retrieved drug samples as per previously published standard methodology. 6,[18][19][20][21][22] Heavy metal concentration was determined by inductively coupled plasma-atomic emission spectrometer (IRIS Intrepid II XSP Duo; Thermo Electron Corp., Munich, Germany). Methodology, chemical standards, reagents, and vials were acquired as per standards set by the United States Environmental Protection Agency, methods 5021A, 8015, 8021, and 8260.…”

Section: Methodology Of Drug Chemical Analysis and Toxicologymentioning

confidence: 99%

Outcomes and Toxicology of Herbal Drugs in Alcoholic Hepatitis – A Single Center Experience from India

Philips¹,

Rajesh²,

George³

et al. 2019

Journal of Clinical and Translational Hepatology

View full text Add to dashboard Cite

Background and Aims: We aimed to study clinical outcomes and liver biopsy features of alcoholic hepatitis (AH) patients on complementary and alternative medicines (CAMs) and to analyze the retrieved drugs for chemical and toxic components linked to drug-induced liver injury. Methods: We retrospectively assessed clinical, biochemical and liver biopsy features of AH patients on CAM with drug-induced liver injury (AH-CAM, n = 27) and compared them to a control group (classical AH, n = 29) on standard of care. Patients without liver biopsy evaluation and other causes for liver disease were excluded. Samples of the CAMs (n = 42) from patients were retrieved and assessed for chemical and toxins. Results: All were males, and significantly worse clinical presentation, biochemical severity, and liver disease scores were notable in patients with AH-CAM. Traditional Ayurvedic-polyherbal formulations were the most commonly used CAM. On liver histology, varying grades of severe-necrosis, severe hepatocellular, canalicular, cholangiolar cholestasis with predominant lymphocytic-portal-inflammation and varying grades of interface-hepatitis were noted in AH-CAM. Analysis of CAMs revealed presence of heavy metals up to 100,000 times above detectable range and adulterants, such as antibiotics, chemotherapy agents, nonsteroidal anti-inflammatory drugs, alcohols, antidepressants, anxiolytics, and recreational drugs. On follow up, a significantly higher number of patients with AH on CAM died at end of 1, 3-and-6-months compared to controls (37% vs. 83%, 29% vs. 62%, 18% vs. 52% respectively; p < 0.001). Conclusions: Patients with AH and CAM-related druginduced liver injury have extremely poor short-term survival in the absence of liver transplantation compared to those patients with AH on evidence-based management. Early transplant referral and educating on and curbing of CAM use in severe liver disease through strict monitoring of unregulated traditional health practices can help ease the burden of liver-related death.

show abstract

“…In recent years, several studies have reported systematic methods based on conventional analytical instruments and novel biosensors. These traditional analytical methods, recognized as the gold standard for determining pesticide residues in food samples, are very effective and fairly accurate; they comprise high-performance liquid chromatography (HPLC) [ 5 , 6 ], gas chromatography [ 7 , 8 ], mass chromatography [ 9 , 10 ], and their combinations [ 11 , 12 ]. Despite the advantages of conventional analytical methods, they are insufficient to be efficiently applied for on-site and rapid detection because of limitations such as time and labor consumption, complex procedures, and expensive equipment.…”

Section: Introductionmentioning

confidence: 99%

Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane

Lim

Park

et al. 2020

Nanomaterials

Self Cite

View full text Add to dashboard Cite

Sol-gel-based mesopores allow the entry of target small molecules retained in their cavity and aptamers to bind to target molecules. Herein, sol-gel-based materials are applied to screen-selective aptamers for small molecules, such as pesticides. To enhance the efficiency of aptamer screening using a sol-gel, it is necessary to increase the binding surface. In this study, we applied the sol-gel to an anodized aluminum oxide (AAO) membrane, and the morphological features were observed via electron microscopy after spin coating. The binding and elution processes were conducted and confirmed by fluorescence microscopy and polymerase chain reaction. The sol-gel coating on the AAO membrane formed a hollow nanocolumn structure. A diazinon-binding aptamer was bound to the diazinon-containing sol-gel-coated AAO membrane, and the bound aptamer was effectively retrieved from the sol-gel matrix by thermal elution. As a proof of concept, a sol-gel-coated AAO disc was mounted on the edge of a pipette tip, and the feasibility of the prepared platform for the systematic evolution of ligands by exponential enrichment (SELEX) of the aptamer binding was also confirmed. The proposed approach will be applied to an automated SELEX cycle using an automated dispenser, such as a pipetting robot, in the near future.

show abstract

Determination of 60 pesticides in hen eggs using the QuEChERS procedure followed by LC-MS/MS and GC-MS/MS

Cited by 109 publications

References 32 publications

Multi-residue analysis of 203 pesticides in strawberries by liquid chromatography tandem mass spectrometry in combination with the QuEChERS method

Multi-residue analysis of 203 pesticides in strawberries by liquid chromatography tandem mass spectrometry in combination with the QuEChERS method

Outcomes and Toxicology of Herbal Drugs in Alcoholic Hepatitis – A Single Center Experience from India

Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane

Contact Info

Product

Resources

About