Determination of 8 Diuretics and Probenecid in Human Urine by Gas Chromatography-Mass Spectrometry: Confirmation Procedure

Abstract

A fast and sensitive method for determination of 8 diuretics (acetazolamide, bendroflumethiazide, bumetanide, chlorthalidone, furosemide, hydrochlorothiazide, metolazone, triamterene) and masking agent (probenecid) in human urine using gas-chromatography with mass spectrometric detection is described. The extraction of the substances as function of the nature of organic solvent, mixing time and pH of aqueous phase was studied. The tandem mass spectrometry was used to increase selectivity of diuretics determination due to elimination of background interferences. Fragmentation reactions were studied for each compound and their collision energies were optimized to obtain the best selectivity. The results of method's validation demonstrate its suitability in routine analysis for confirmation purposes.

Noble metal nanostructures in optical biosensors: Basics, and their introduction to anti-doping detection

“…Probenecid (p-dipropylsulphamyl) benzoic acid (PB) was assayed for the first time by Lorenzo et al using SERS-active AgNPs (average diameter 60 nm). They reported the LOD of PB determined by SERS as low as 1.2 × 10 3 ng/mL [157] which was in the range of the published value using GC-MS (50 ng/mL) [158]. This sensitivity was lower compared to the one resulted with the fast-GC and fast-GC/MS techniques (10 ng/mL) [156].…”

Molecular modelling and synthesis of a polymer for the extraction of amiloride and triamterene from human urine

“…These methods provide the necessary selectivity and sensitivity. [4][5][6][7] The other methods for determination of triamterene and amiloride include spectrophotometry and uorimetry. [8][9][10][11][12][13][14] However, direct analysis of urine samples is complicated because the complex urine matrix interferes with the determination of analytes.…”

Determination of Volatiles in Mouse Urine by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry

“…Of particular importance are volatile and semi-volatile substances functioning as pheromones. Examples of the extraction of volatile compounds in both rodent and human urine have been reported in literature using different techniques such as purge-and-trap (Prieto et al 2000) and organic solvents (Achiraman et al 2010;Zaporozhets, Tsyrulneva, and Ischenko 2012;Hardt and Angerer 2000;Zhang et al 2008). On the other hand, solid-phase microextraction (SPME) has gained popularity as a simple, selective, and environmentally friendly tool for the sampling of a variety of volatile and semivolatile compounds in different matrices.…”