Determination of a Chemical Marker in Dipterocarpus alatus Oleoresin Samples and Bioactivity Screening via Antioxidants, Nitric Oxide Inhibition on Murine RAW 264.7 Cells, and Collagen Production on Normal Human Dermal Fibroblasts

Abstract

Bioactivities of oleoresin such as antibacterial, antifungal, anticancer, anti-inflammatory activity, insecticidal activity, antioxidant activities, antiarthritic agent, 3 including its use for the treatment of skin allergies, diarrhea, dysentery, astringency have been reported. 4,5 The essential oils, components separated from oleoresins were utilized in pharmaceutical industry, perfume industry, food, drink additives and other chemical industries. 6 The D. alatus oleoresin turns brown after standing at room temperature for a few days while D. alatus oleoresin after boiling appears as a clear homogeneous yellow liquid and are more stable without a precipitate. The oleoresin could improve the quality by degumming under alkaline condition at temperature of 95 ºC for 30 min according to the announcement of the Department of Energy, Thailand. 7 The degumming process facilitates the removal of impurities or gum from the oil to prevent crude oil settling during storage or transport. However, the alkali degumming products could change the chemical composition subsequently affect biological activities. The properties of D. alatus oleoresin were found to have potential as an alternative liquid biofuel and (-)-α-gurjunene was found as the major compound in oleoresin by GC-MS analysis. 8 The major compound in D. alatus oleoresin is (-)-α-gurjunene which can be used as a chemical marker. Copaifera langsdorffii was used to treat rat skin wounds and also had potential in healing wounds of the skin. 9

The characteristics and biological activity enhancements of melatonin encapsulations for skin care product applications

“…The absorbance of the sample was measured at 540 nm. The collagen content was calculated and compared with the calibration curve of standard collagen type I ( Longsri et al, 2021 ).…”