Determination of a Hair Dye Using Different Derivatization Agents and HPLC in Combination with UV, Electrochemical, and Fluorescence Detection

Voltammetric investigation of hair dye constituents: application to the quantification of p-phenylenediamine

“…The multi-ingredient composition of hair dyes poses a problem to the analytical chemist and as such its determination is often reliant upon separation techniques. Although the determination of PPD in hair dyes has been achieved by gas chromatography, 11,18 capillary electrophoresis 19 and mass spectrometry, 18 its detection has been mostly based on high performance liquid chromatography (HPLC), [20][21][22][23][24][25][26][27] in conjunction with diode array, 20 UV, 21,22 chemiluminescence, 23 fluorescence 22 and electrochemical detection. 22,24,25 The aim of this work was to investigate the applicability of direct electroanalysis as a means of producing a facile route through which the PPD concentration in hair dye formulations can be monitored.…”

“…The method we describe, using a bare unmodified glassy carbon electrode, is a fast and simple technique of practical interest, with no preliminary extraction step, and contrasts with the majority of the HPLC methods. [20][21][22][23][24][25][26][27] This report serves to show that by means of adapting the electrochemical approach the problem of electroactive interference can be overcome.…”