Determination of a hydrophilic paclitaxel derivative, 7‐xylosyl‐10‐deacetylpaclitaxel in rat plasma by LC–MS/MS

Abstract

A LC-MS/MS method for the determination of a hydrophilic paclitaxel derivative 7-xylosyl-10-deacetylpaclitaxel in rat plasma was developed to evaluate the pharmacokinetics of 7-xylosyl-10-deacetylpaclitaxel in the rats. 7-Xylosyl-10-deacetylpaclitaxel and docetaxel (IS for 7-xylosyl-10-deacetylpaclitaxel) were extracted from rat plasma with acetic ether and analyzed on a Hypersil C(18 )column (4.6 x 150 mm i.d., particle size 5 microm) with the mobile phase of ACN/0.05% formic acid (50:50, v/v). The analytes were detected using an ESI MS/MS in the multiple reaction monitoring mode. The standard curves for 7-xylosyl-10-deacetylpaclitaxel in plasma were linear (r > 0.999) over the concentration range of 2.0-1000 ng/mL with a weighting of 1/concentration(2). The method showed a satisfactory sensitivity (2.0 ng/mL using 50 microL plasma), precision (CV <or= 10.1%), accuracy (relative error -12.4 to 12.0%), and selectivity. This method was successfully applied to the pharmacokinetic study of 7-xylosyl-10-deacetylpaclitaxel in rat plasma after intravenous administration of 7-xylosyl-10-deacetylpaclitaxel to female Wistar rats.

Pharmacokinetic and tissue distribution of paclitaxel in rabbits assayed by LC‐UV after intravenous administration of its novel liposomal formulation

“…To our knowledge, rabbit tissue samples pre‐treated using the PPT method have not been reported. In the recent literature on PPT method in rabbit plasma (Jiang et al ., ), 100 μL aliquots of working solutions were transferred to clean dry centrifugation tubes and 0.5 mL of rabbit plasma was added to each tube. Then 0.4 mL of acetonitrile was added.…”

“…Thus, it is necessary to develop and validate an analysis method to investigate the pharmacokinetics and tissue distribution of the novel liposomal formulation of PTX. A number of analytical methods of determining PTX in biological fluids and tissues have been reported, including liquid chromatography–mass spectrometry (LC‐MS), in human, dog, rat, mouse plasma or tissues (Tan et al ., ; Gardner et al ., ; Bulitta et al ., ; Jiang et al ., ; Hou et al ., ; Grobosch et al ., ; NageswaraRao et al ., ; Zhang et al ., ), and high‐performance liquid chromatography (HPLC) in plasma (Suno et al ., ; Mowafy et al ., ). Among these analytical methods, HPLC has been most frequently employed in the pharmacokinetic studies of PTX owing to its simplicity, sensitivity and selectivity.…”

Quantification of Taxanes in Biological Matrices: A Review of Bioanalytical Assays and Recommendations for Development of New Assays

“…During development of multiple assays, the response for ESI in positive and negative mode is compared. For docetaxel [84,90,94], felotaxel [98], paclitaxel [66,67] and 7-xylosyl-10-deacetylpaclitaxel [101] it appears that ionization in the positive mode results in an optimal analytical response. Negative ion mode resulted in higher responses of IDN5390 and ortotaxel [103].…”

“…For quantification of docetaxel [83][84][85][86][87][88][89][90], docetaxel and its metabolites [91,92] (SupplemenTary Table 4), or docetaxel and other drugs [93][94][95] (Supplemen- Tary Table 5), fewer assays are published. A limited number of LC-MS/MS assays are developed for the quantification of the second-generation taxanes cabazitaxel [96,97], felotaxel [98,99] and larotaxel [100] and other taxane analogues (Sup-plemenTary Table 6) [101][102][103][104][105]. Usually, tandem quadrupole MS is performed, but assays using MS with a single quadrupole have also been developed for the quantification of paclitaxel and docetaxel [59,81].…”

LC‐ESI‐MS/MS determination of paclitaxel on dried blood spots

“…Several pharmacokinetic studies of PAC have been published as well as analytical methods for its determination in various biological fluids using HPLC with UV (Sparreboom et al , ; Martin N et al, ; Lee et al , ; Mader et al , ; Wang et al , ; Kim et al , ; Suno et al , ; Yonemoto et al , ) and mass spectrometric detection (Liu et al , ; Guo et al , ; Alexandera et al , ; Gao et al , ; Mortier and Lambert, ; Thijssen et al , ; Yua et al , ; Jiang et al , ; Zhang and Chen, ).…”