Determination of a Tentative Epidemiological Cut-Off Value (ECOFF) for Dalbavancin and Enterococcus faecium

Weber, Robert E.; Fleige, Carola; Layer, Franziska; Neumann, Bernd; Kresken, Michael; Werner, Guido

doi:10.3390/antibiotics10080915

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…Essentially, the ECOFF value determines the threshold between wild type susceptible strains and those with acquired resistance [34]. ECOFFs are statistically determined from collated data derived from thousands of AST distributions [35]. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) mostly relies on statistical programs-such as ECOFFinder-to determine an ECOFF value [36].…”

Section: Introductionmentioning

confidence: 99%

Profiling Antibiotic Susceptibility among Distinct Enterococcus faecalis Isolates from Dental Root Canals

Manoil,

Cerit,

Fang

et al. 2023

Antibiotics

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Enterococcus faecalis, a leading multi-resistant nosocomial pathogen, is also the most frequently retrieved species from persistently infected dental root canals, suggesting that the oral cavity is a possible reservoir for resistant strains. However, antimicrobial susceptibility testing (AST) for oral enterococci remains scarce. Here, we examined the AST profiles of 37 E. faecalis strains, including thirty-four endodontic isolates, two vanA-type vancomycin-resistant isolates, and the reference strain ATCC-29212. Using Etest gradient strips and established EUCAST standards, we determined minimum inhibitory concentrations (MICs) for amoxicillin, vancomycin, clindamycin, tigecycline, linezolid, and daptomycin. Results revealed that most endodontic isolates were susceptible to amoxicillin and vancomycin, with varying levels of intrinsic resistance to clindamycin. Isolates exceeding the clindamycin MIC of the ATCC-29212 strain were further tested against last-resort antibiotics, with 7/27 exhibiting MICs matching the susceptibility breakpoint for tigecycline, and 1/27 reaching that of linezolid. Both vanA isolates confirmed vancomycin resistance and demonstrated resistance to tigecycline. In conclusion, while most endodontic isolates remained susceptible to first-line antibiotics, several displayed marked intrinsic clindamycin resistance, and MICs matched tigecycline’s breakpoint. The discovery of tigecycline resistance in vanA isolates highlights the propensity of clinical clone clusters to acquire multidrug resistance. Our results emphasize the importance of implementing AST strategies in dental practices for continued resistance surveillance.

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Section: Introductionmentioning

confidence: 99%

Profiling Antibiotic Susceptibility among Distinct Enterococcus faecalis Isolates from Dental Root Canals

Manoil,

Cerit,

Fang

et al. 2023

Antibiotics

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show abstract

“…For species with sufficient isolates and excellent inhibitory activity as demonstrated by BDQ/CLO, ECOFFs were identified using ECOFFinder (EUCAST) ( 34 ). For the unimodal MIC distribution profile, the ECOFF was defined as the concentration that could inhibit 95% of the bacterial population.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Susceptibility Testing Using the MYCO Test System and MIC Distribution of 8 Drugs against Clinical Isolates of Nontuberculous Mycobacteria from Shanghai

Ying

Yang

et al. 2023

Microbiol Spectr

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We designed customized panel that contains 8 repurposed drugs, including vancomycin (VAN), bedaquiline (BDQ), delamanid (DLM), faropenem (FAR), meropenem (MEM), clofazimine (CLO), cefoperazone-avibactam (CFP-AVI), and cefoxitin (FOX) from the MYCO test system. To better understand the efficacy of these 8 drugs against different NTM species, we determined the MICs of 241 NTM isolates collected in Shanghai, China.

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“…For species with sufficient isolates and excellent inhibitory activity as demonstrated by BDQ/CFZ, ECOFFs were identified using ECOFFinder (EUCAST) (15). For the unimodal MIC distribution profile, ECOFF was defined as the concentration that could inhibit 95% of the bacterial population.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial susceptibility testing using MYCO test-system and MIC distribution of 8 drugs against clinical isolates from Shanghai of Nontuberculous Mycobacteria

Ying

Yang

Wang

2022

Preprint

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Given the increased incidence and prevalence of nontuberculous mycobacteria (NTM) diseases and the natural resistance of NTM to multiple antibiotics, in vitro susceptibility testing of different NTM species against drugs from the MYCO test system and new applied drugs are required. 241 NTM clinically isolates were under analyzed, including 181 slowly growing mycobacterium (SGM) and 60 rapidly growing mycobacterium (RGM). The Sensititre SLOMYCO and RAPMYCO panels were used for the drug susceptibility testing to commonly used anti-NTM antibiotics. Furthermore, Minimum inhibitory concentration (MIC) distributions were determined against 8 potential anti-NTM drugs, including vancomycin (VA), bedaquiline (BDQ), delamanid (DLM), faropenem (FAR), meropenem (MPM), clofazimine (CFZ), avibactam (CAZ), and Cefoxitin (FOX) and epidemiological cut-off values (ECOFFs) were analyzed using ECOFFinder. The results showed that most of the SGM strains were susceptible to clarithromycin (CLA), rifampicin (RFB) from the SLOMYCO panels and BDQ, CFZ from the 8 applied drugs, while, RGM strains were susceptible to tigecycline (TGC) from the RAPMYCO panels and also BDQ, CFZ. The ECOFF values of CFZ were 0.25μg/ml, 0.25μg/ml, 0.5μg/ml, and 1μg/ml for M. kansasii, M. avium, M. intracellulare, and M. abscessus, respectively, and BDQ was 0.5μg/ml for the same four prevalent NTM species. Due to the weak activity of the other 6 drugs, no ECOFF was determined. This study on the susceptibility of NTM includes 8 potential anti-NTM drugs and a large sample size of Shanghai clinical isolates. and demonstrated that BDQ and CFZ had efficient activities against different NTM species in vitro, which can be applied for the treatment of NTM diseases.

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Determination of a Tentative Epidemiological Cut-Off Value (ECOFF) for Dalbavancin and Enterococcus faecium

Cited by 12 publications

References 36 publications

Profiling Antibiotic Susceptibility among Distinct Enterococcus faecalis Isolates from Dental Root Canals

Profiling Antibiotic Susceptibility among Distinct Enterococcus faecalis Isolates from Dental Root Canals

Antimicrobial Susceptibility Testing Using the MYCO Test System and MIC Distribution of 8 Drugs against Clinical Isolates of Nontuberculous Mycobacteria from Shanghai

Antimicrobial susceptibility testing using MYCO test-system and MIC distribution of 8 drugs against clinical isolates from Shanghai of Nontuberculous Mycobacteria

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