Determination of abscisic acid following Paleg's method

Sívori, E. M.; Sonvico, V.; Fernández, Nola

doi:10.1093/oxfordjournals.pcp.a074704

Cited by 11 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Others are long and laborious such as the rice seedling (7), oat mesocotyl (8 ), and cotton explant (1) bioassays. Still others have a limited sensitive range (7,9,13). Milborrow (8 ), in his recent review of ABA bioassays, considers the cereal coleoptile straight growth bioassays (5) the most fre quently used ABA bioassays.…”

mentioning

confidence: 99%

Two Bioassay Techniques for Determining Abscisic Acid Concentrations1

Bakken

Boe

1982

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Two bioassay techniques for determining abscisic acid (ABA) concentrations are reported. One used bean (Phaseolus vulgaris) embryos while the other used lettuce (Lactuca sativa) hypocotyls. The bean embryo assay requires that seeds first be soaked over night, then the embryos excised and placed in known concentrations of (RS)-ABA for 24 hours, and finally measured. The lettuce hypocotyl assay utilizes seeds incubated for 48 hours, then placed in known concentrations of (RS)-ABA for 72 hours and finally measured. A dose-response curve of each bioassay may then be used to determine unknown concentrations of ABA. The bean embryo test was more rapid, but the lettuce hypocotyl assay was simpler and more sensitive. Elongation of bean embryos and lettuce hypocotyls was inversely correlated to the log concentration of (RS)-ABA within the range of 0.01 to 100 and 0.001 to 100 μg/ml, respectively.

show abstract

mentioning

confidence: 99%