Determination of acrolein in serum by high‐performance liquid chromatography with fluorescence detection after pre‐column fluorogenic derivatization using 1,2‐diamino‐4,5‐dimethoxybenzene

Imazato, Takahiro; Kanematsu, Mariko; Kishikawa, Naoya; Ohyama, Kaname; Hino, Takako; Ueki, Yukitaka; Maehata, Eisuke; Kuroda, Naotaka

doi:10.1002/bmc.3422

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…SRM/MRM MS or label-free ; sample derivatization with AIDA assay, 4-APC) [[186], [187], [188],194,195] GC-MS/MS (sample derivatization with hydrazine-based or non-hydrazine-based reagents) [178,179,190,191,196] CE-UV [193]Atherosclerosis [112]Coronary and peripheral artery disease [196,311]Aortic stenosis [265]Heart failure [312]Apolipoprotein B-100 (LDLs) [43]Apolipoprotein AI (HDLs) [313]Mitochondrial proteins [314]Carnosine [282,285]Lipid peroxidation-derived aldehydesACR Free ACR : Fluorescence methods (e.g. Skraup reaction with m-aminophenol, lanthanide probe) [202,204,205]LC-FD [206,208]LC-MS/MS (sample derivatization) [214] ACR-protein adducts : Immunochemical methods [209,211]GC-MS/MS and LC-MS/MS (e.g. SRM/MRM MS; enrichment strategies, e.g.…”

Section: Methods For Identification and Quantitation Of Lipid Peroxidmentioning

confidence: 99%

“…A less expensive and practical fluorescence-based method, using a two-step tethering strategy, for the detection of ACR in human plasma under mild conditions without the use of HPLC, has been also developed [205]. In other studies, HPLC-fluorescence detection in combination with a derivatization reaction was used for the accurate quantification of ACR in human serum and plasma samples [[206], [207], [208]].…”

Section: Methods For Identification and Quantitation Of Lipid Peroxidmentioning

confidence: 99%

“…MS-based methods were successfully applied to determine ACR using GC-MS [212] or LC-MS/MS [134,213] and mostly DNPH as a derivatization reagent [214]. However, Imazato et al developed a method for ACR analysis by HPLC with fluorescence detection after pre-column derivatization using 1,2-diamino-4,5-dimethoxybenzen (DDB) [208], which is not a hydrazine reagent and does not have intrinsic fluorescence itself that can interfere with the detection of ACR derivative.…”

Section: Methods For Identification and Quantitation Of Lipid Peroxidmentioning

confidence: 99%

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Lipoxidation in cardiovascular diseases

et al. 2019

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Lipids can go through lipid peroxidation, an endogenous chain reaction that consists in the oxidative degradation of lipids leading to the generation of a wide variety of highly reactive carbonyl species (RCS), such as short-chain carbonyl derivatives and oxidized truncated phospholipids. RCS exert a wide range of biological effects due to their ability to interact and covalently bind to nucleophilic groups on other macromolecules, such as nucleic acids, phospholipids, and proteins, forming reversible and/or irreversible modifications and generating the so-called advanced lipoxidation end-products (ALEs).Lipoxidation plays a relevant role in the onset of cardiovascular diseases (CVD), mainly in the atherosclerosis-based diseases in which oxidized lipids and their adducts have been extensively characterized and associated with several processes responsible for the onset and development of atherosclerosis, such as endothelial dysfunction and inflammation.Herein we will review the current knowledge on the sources of lipids that undergo oxidation in the context of cardiovascular diseases, both from the bloodstream and tissues, and the methods for detection, characterization, and quantitation of their oxidative products and protein adducts.Moreover, lipoxidation and ALEs have been associated with many oxidative-based diseases, including CVD, not only as potential biomarkers but also as therapeutic targets. Indeed, several therapeutic strategies, acting at different levels of the ALEs cascade, have been proposed, essentially blocking ALEs formation, but also their catabolism or the resulting biological responses they induce. However, a deeper understanding of the mechanisms of formation and targets of ALEs could expand the available therapeutic strategies.

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Section: Methods For Identification and Quantitation Of Lipid Peroxidmentioning

confidence: 99%

Section: Methods For Identification and Quantitation Of Lipid Peroxidmentioning

confidence: 99%

Section: Methods For Identification and Quantitation Of Lipid Peroxidmentioning

confidence: 99%

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Lipoxidation in cardiovascular diseases

et al. 2019

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“…The aromatic diamine analogue 1,2-diamino-4,5-dimethoxybenzene (DDB, 131) has been reported as an effective reagent for the fluorometric detection of aromatic aldehydes, 329 acrolein, 330 and methylglyoxal. 331 Derivatizations either required strongly basic conditions at room temperature or heating to 80 °C in DMSO.…”

Section: Imidazole Formationmentioning

confidence: 99%

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

et al. 2023

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Many successful stories in enzyme engineering are based on the creation of randomized diversity in large mutant libraries, containing millions to billions of enzyme variants. Methods that enabled their evaluation with high throughput are dominated by spectroscopic techniques due to their high speed and sensitivity. A large proportion of studies relies on fluorogenic substrates that mimic the chemical properties of the target or coupled enzymatic assays with an optical read-out that assesses the desired catalytic efficiency indirectly. The most reliable hits, however, are achieved by screening for conversions of the starting material to the desired product. For this purpose, functional group assays offer a general approach to achieve a fast, optical read-out. They use the chemoselectivity, differences in electronic and steric properties of various functional groups, to reduce the number of false-positive results and the analytical noise stemming from enzymatic background activities. This review summarizes the developments and use of functional group probes for chemoselective derivatizations, with a clear focus on screening for enzymatic activity in protein engineering.

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“…In addition, o-phenylenediamine (OPD) reagents such as 1,2-diamine-4,5dimethoxybenzene (DDB) and 4-methoxy-o-phenylenediamine (MPD) were used for derivatization of ACR or α-oxoaldehydes [52][53][54]. Besides, 2-thiobarbituric acid (TBA) was applied for the FL derivatization of dialdehyde such as malondialdehyde (MDA) [55].…”

Section: Hplc-flmentioning

confidence: 99%