Determination of action spectrum for sparfloxacin‐photosensitized single‐strand breaks in plasmid pBR322 DNA

Sayama, Katsuhide; Kobayashi, Yuki; Fujita, Hitoshi; Ito, Atsushi; Tokura, Y.; Sasaki, Masako

doi:10.1111/j.1600-0781.2005.00190.x

Cited by 4 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cleavage: Almost all FQs have been reported to induce SSB on plasmid DNA: ENO (18,37,52,87,(108)(109)(110)(111), PEF (18,108,109), BAY (40,81,106), LOM (37,40,81,87,108,109,(111)(112)(113), NOR (18,29,87,108,109,111), SPA (37,87,108,109,112,114), OFL (37,39,45,87,108,109,111), CIP (37,40,87,108,109,(111)(112)(113), LEV…”

Section: Nucleic Acidsmentioning

confidence: 99%

“…Cleavage : Almost all FQs have been reported to induce SSB on plasmid DNA: ENO (18,37,52,87,108–111), PEF (18,108,109), BAY (40,81,106), LOM (37,40,81,87,108,109,111–113), NOR (18,29,87,108,109,111), SPA (37,87,108,109,112,114), OFL (37,39,45,87,108,109,111), CIP (37,40,87,108,109,111–113), LEV (45,112), RUF (70,108,109), FLE (108,109,111), TOS (87,111), ORB, CLI (108,109), MOX (40,45) and GAT (87). As observed for the other FQs DNA targets, most of 6,8 dihalogenated FQs (LOM, FLE, ORB, BAY and SPA) show a higher potential as DNA cleaving agents; also in this case methoxy substitution (MOX) tends to reduce this effect (40,45).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Photosensitization Reactions of Fluoroquinolones and Their Biological Consequences

Guidi

Bracchitta

Catalfo

2011

Photochem & Photobiology

View full text Add to dashboard Cite

This review focuses on damage photosensitized by the fluoroquinolone (FQ) antibacterial drugs. Different models are employed to study biosubstrate photodamage mediated by FQs (organisms, cells, isolated biomolecules and super molecules). Being that the effect of environment (polarity of the medium, ions, pH, binding with bio-molecules, etc.) is crucial in FQ photochemistry, photobiological reactions can be consequently dramatically influenced. Thus, the photosensitization processes induced by FQs are here discussed taking into account that such extensive and cross-targeted pathological implications request an excursus covering photosensitization in systems of increasing molecular complexity. In vivo and in vitro evidences for photoallergy, phototoxicity, photomutagenesis and photocarcinogenesis mediated by FQs are discussed.

show abstract

Section: Nucleic Acidsmentioning

confidence: 99%

mentioning

confidence: 99%

Photosensitization Reactions of Fluoroquinolones and Their Biological Consequences

Guidi

Bracchitta

Catalfo

2011

Photochem & Photobiology

View full text Add to dashboard Cite

show abstract

“…Nonetheless, although ocular (cornea) phototoxicity was observed in LE rats treated with 8-MOP, the detection of retinal phototoxicity from systemic drugs that absorb light above 400 nm requires use of a visible light source. Because SPFX has an absorption band in the range of 320 to 450 nm, 19 the lack of retinal phototoxicity after treatments with SPFX in this study may relate to the use of a UV source. 8-Methosypsoralen, LMFX, and PFD do not absorb visible light.…”

Section: Discussionmentioning

confidence: 92%

Assessment of 8-Methosypsoralen, Lomefloxacin, Sparfloxacin, and Pirfenidone phototoxicity in Long-Evans Rats

Adachi¹,

Yuko²,

Satou³

et al. 2014

Int J Toxicol

View full text Add to dashboard Cite

Phototoxicity has a strong impact on drug development. Although several animal models have been developed to quantitatively assess human risks, none have been validated for standardized use. In this study, we validated an in vivo phototoxicity model using Long-Evans (LE) rats treated with 4 well-known phototoxic drugs, namely 8-methoxypsoralen, lomefloxacin, sparfloxacin, and pirfenidone. Daily macroscopic observations of skin and eyes, ophthalmological examinations 4 days after dosing, and blood sampling for toxicokinetics (TKs) were performed after exposure of treated animals to ultraviolet, and dose-dependent eye and/or skin reactions were noted for all compounds. Margins of safety were calculated when possible and correlated well with known relative phototoxicity of the 4 compounds. We conclude that the present in vivo phototoxicity assay using LE rats with TK analysis can be used to quantitatively predict the risk of pharmaceutical phototoxicity in humans.

show abstract

“…Numerous FQs have been reported to induce SSB on plasmid DNA (7,11,14,16–32); the results are shown in Table 2. As a general trend, FQs with a fluorine at C‐8 (LOM, FLE, ORB, BAY and SPA) show a higher potential as DNA cleaving agents, while methoxy substitution (MOX) tends to reduce this effect.…”

Section: Detection Of Dna Damage Photosensitized By Fluoroquinolonesmentioning

confidence: 99%

Photosensitized DNA Damage: The Case of Fluoroquinolones^†

Lhiaubet‐Vallet

Boscá

Miranda³

2009

Photochem & Photobiology

View full text Add to dashboard Cite

This review focuses on DNA damage photosensitized by the fluoroquinolone (FQ) antibacterial drugs. The in vivo evidence for photocarcinogenesis mediated by FQs is presented in the introduction. The different methods employed for detection of DNA-photodamage mediated by FQs are then summarized, including gel electrophoresis (with whole cells, with isolated DNA and with oligonucleotides) and chromatographic analysis (especially HPLC with electrochemical and MS ⁄ MS detection). The chemical mechanisms involved in the formation of the reported lesions are discussed on the basis of product studies and transient spectroscopic evidence. In general, the literature coverage is limited to the last decade, although some earlier citations are also included.

show abstract

Determination of action spectrum for sparfloxacin‐photosensitized single‐strand breaks in plasmid pBR322 DNA

Abstract: The findings suggest that protection of skin from short wavelengths of visible light beyond the absorption wavelengths as well as UVA light is of primary importance in prevention against induction of SPFX phototoxicity.

Cited by 4 publications

References 24 publications

Photosensitization Reactions of Fluoroquinolones and Their Biological Consequences

Photosensitization Reactions of Fluoroquinolones and Their Biological Consequences

Assessment of 8-Methosypsoralen, Lomefloxacin, Sparfloxacin, and Pirfenidone phototoxicity in Long-Evans Rats

Photosensitized DNA Damage: The Case of Fluoroquinolones^†

Contact Info

Product

Resources

About

Determination of action spectrum for sparfloxacin‐photosensitized single‐strand breaks in plasmid pBR322 DNA

Abstract: The findings suggest that protection of skin from short wavelengths of visible light beyond the absorption wavelengths as well as UVA light is of primary importance in prevention against induction of SPFX phototoxicity.

Cited by 4 publications

References 24 publications

Photosensitization Reactions of Fluoroquinolones and Their Biological Consequences

Photosensitization Reactions of Fluoroquinolones and Their Biological Consequences

Assessment of 8-Methosypsoralen, Lomefloxacin, Sparfloxacin, and Pirfenidone phototoxicity in Long-Evans Rats

Photosensitized DNA Damage: The Case of Fluoroquinolones†

Contact Info

Product

Resources

About

Photosensitized DNA Damage: The Case of Fluoroquinolones^†