Determination of acyclovir by ultrafiltration and high-performance liquid chromatography

Nebinger, Peter; Koel, Marlies

doi:10.1016/0378-4347(93)80128-q

Cited by 29 publications

(13 citation statements)

References 10 publications

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“…Although the sensitivity of these assays with fluorometric detection was high, with a lower limit of quantification (LLOQ) of 0.02 µg/mL, the procedure for pre-treatment of biological samples was tedious, and sometimes pre-column derivatization had to be performed (Tsuchie et al, 2001). In addition to fluorometric detection, HPLC with UV detection for acyclovir, ganciclovir and penciclovir at a fixed wavelength (254 nm) was applied in most reports published (Nebinger and Koel, 1993;Page et al, 1996;Boulieu et al, 1997;Campanero et al, 1998;Pham-Huy et al, 1999;He et al, 2000;Merodio et al, 2000;Brown et al, 2002). A relatively high sensitivity of the assays (LLOQ 0.05-0.1 µg/mL) for these guanosine analogues in biological matrices was achieved, owing to the maximum UV absorbance at approximately 252-254 nm.…”

Section: Discussionmentioning

confidence: 99%

Determination of antiviral nucleoside analogues AM365 and AM188 in perfusate and bile of the isolated perfused rat liver using HPLC

Wang

Nation

Evans

et al. 2005

Biomedical Chromatography

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Development, validation and application of an HPLC assay for new antiviral nucleoside analogues AM365 and AM188 in isolated perfused rat liver perfusate and bile were performed. An analytical column (Phenosphere-NEXT, 250 x 4.6 mm, C(18), 4 microm, Phenomenex) was used in tandem with a guard column (4 x 3 mm, C(18), Phenomenex) and operated at 25 degrees C. The mobile phase [methanol:10 mmol/L sodium orthophosphate buffer (pH 7.0), 15:85, v/v] was pumped at 1 mL/min. The signal from a diode array detector was collected from 190 to 300 nm. The chromatogram was processed at 220 and 252 nm for AM365 and AM188, respectively. The HPLC method was validated by six intraday and seven interday runs. Standard curves were linear in the range 0.125-8.00 microg/mL for AM365 and AM188, and the lower limit of quantification for AM365 and AM188 was 0.125 microg/mL. Mean interday precision and accuracy of IPL perfusate quality control samples were within 8.8%, and mean intraday precision and accuracy were within 13.1%. The assay has been successfully used in the study of metabolism and disposition of AM365 in the isolated perfused rat liver.

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Section: Discussionmentioning

confidence: 99%

Determination of antiviral nucleoside analogues AM365 and AM188 in perfusate and bile of the isolated perfused rat liver using HPLC

Wang

Nation

Evans

et al. 2005

Biomedical Chromatography

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“…If protein binding proves to be low, ultrafiltration as a means of deproteinization of the serum samples can be applied. Although ultrafiltration is commonly used for determining free unbound drug levels in serum [21], it has previously been applied as a means of sample preparation for other polar compounds such as acyclovir, ganciclovir, ribavirin and zidovudine [13,[22][23][24][25]. The obtained ultra filtrate is purely aqueous which is particularly suitable for injecting onto LC-systems that are equilibrated under the same conditions.…”

Section: Protein Binding and Ultrafiltrationmentioning

confidence: 99%

Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Sturkenboom¹,

Lijke²,

Jongedijk³

et al. 2015

J Appl Bioanal

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Clinical studies on tuberculosis have shown a correlation between low drug exposure and treatment failure and acquired drug resistance. Objective was to develop a LC-MS/MS method for the quantification of isoniazid, pyrazinamide and ethambutol. Stable isotope-labelled isoniazid-D4 and ethambutol-D4 were used as internal standards. Protein binding of isoniazid, pyrazinamide and ethambutol was investigated and proved low. Therefore, sample preparation using ultrafiltration could be applied, resulting in linear calibration curves in the range of 0.2-8 mg/L for isoniazid and ethambutol and 2-80 mg/L for pyrazinamide. The method was validated according to the guidelines of the FDA. A fast, simple and reliable LC-MS/MS method has been developed for the simultaneous determination of isoniazid, pyrazinamide and ethambutol in human serum for therapeutic drug monitoring and pharmacokinetic studies.

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“…This compound is structurally related to acyclovir and endogenous guanosine was not detectable in drugfree patient plasma samples. While there have been two previous reports that have utilized guanosine as internal standard (Jankowski et al, 1998;Nebinger and Koel, 1993), most of the other studies have not employed an internal standard.…”

Section: Stabilitymentioning

confidence: 99%

“…There have been a number of previously published assays for acyclovir, including radioimmunoassay (RIA; Wood et al, 1994), enzyme-linked immunosorbent assay (Tadepalli et al, 1986) and high-performance liquid chromatography (HPLC) using UV (Bahrami et al, 2005;Bangaru et al, 2000;Basavaiah et al, 2003;Boulieu et al, 1997;Caamano et al, 1999;Fernandez et al, 2003;McMullin et al, 1996;Nebinger and Koel, 1993;Pham-Huy et al, 1999;Poirier et al, 1999;Swart et al, 1994;Teshima et al, 2003;Tzanavaras and Themelis, 2007;Vo et al, 2002;Volpato et al, 1997) or fluorescence detection (Jankowski et al, 1998;Mascher et al, 1992;Peh and Yuen, 1997;Svensson et al, 1997). The details of the HPLC assays are summarized in Table 1.…”

Section: Introductionmentioning

confidence: 99%

HPLC‐fluorescence assay for acyclovir in children

Zeng

Nath

Shaw

et al. 2008

Biomedical Chromatography

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A simple, accurate, reliable and sensitive HPLC method was developed and validated for quantitating acyclovir in human plasma. Sample (100 microL) preparation involved addition of guanosine (internal standard) and protein precipitation with 7% perchloric acid and centrifugation. Supernatant (20 microL) was injected onto a C18 HPLC column with a mobile phase of 0.05 m sodium phosphate buffer-acetonitrile (pH 2.35, 992:8, v/v) with 25 microL of 0.4 m tetrabutylammonium hydroxide titrant and fluorescence detection (excitation, 260 nm; emission, 375 nm). Analyte recovery was 101% and the assay response was linear over the acyclovir concentration range of 0.1-20 mg/L. Intra- and inter-day accuracy and precision were less than 7%. The limit of detection and limit of quantitation were 0.033 and 0.1 mg/L, respectively. In five paediatric oncology patients administered intravenous acyclovir, concentrations ranged from 0.24 to 43.65 mg/L. This method can be used to measure acyclovir concentrations in paediatric patients.

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Determination of acyclovir by ultrafiltration and high-performance liquid chromatography

Cited by 29 publications

References 10 publications

Determination of antiviral nucleoside analogues AM365 and AM188 in perfusate and bile of the isolated perfused rat liver using HPLC

Determination of antiviral nucleoside analogues AM365 and AM188 in perfusate and bile of the isolated perfused rat liver using HPLC

Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

HPLC‐fluorescence assay for acyclovir in children

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