Determination of acyclovir in human serum by high-performance liquid chromatography using liquid–liquid extraction and its application in pharmacokinetic studies

Bahrami, Gh.; Mirzaeei, S H; Kiani, Amir

doi:10.1016/j.jchromb.2004.11.038

Cited by 60 publications

(47 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initially, HPLC-UV for quantification of ACV in horse plasma was tested. The use of a simple deproteinization step or a liquid-liquid extraction with dichloromethane/isopropyl alcohol (50:50, v/v) (Bahrami et al, 2005) was not sufficient as clean-up at a detection wavelength of 254 nm. Therefore, deproteinization with PFPA, trifluoroacetic acid or 20% trichloroacetic acid before the liquid-liquid extraction was tested.…”

Section: Resultsmentioning

confidence: 99%

“…The method for the determination of the total concentration of ACV in horse plasma was developed based on methods described in literature (Fernández et al, 2003;Bahrami et al, 2005). To 1.0 mL of plasma, 50 μL of the internal standard solution (1 μg mL −1 for concentrations from 50 to 2500 ng mL…”

Section: Sample Preparationmentioning

confidence: 99%

“…HPLC with UV detection (Fernández et al, 2003;Merodio et al, 2000;Stagni et al, 2004;Bangaru et al, 133 2000;Bahrami et al, 2005;Boulieu et al, 1997;Basavaiah et al, 2003;Poirier et al, 1999;Pham-Huy et al, 1999;de Jalón et al, 2002) is a very common method used for the determination of the drug; however, some of these methods present a detection limit higher than 100 ng mL −1 , which is not sensitive enough to perform PK studies (Stagni et al, 2004;Basavaiah et al, 2003;Pham-Huy et al, 1999). To improve sensitivity, fluorescence detection with very acid mobile phases can be used.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of acyclovir in horse plasma and body fluids by high‐performance liquid chromatography combined with fluorescence detection and heated electrospray ionization tandem mass spectrometry

Maes

Garré

Desmet

et al. 2008

Biomedical Chromatography

View full text Add to dashboard Cite

Two methods are presented for the determination of 'respectively' the plasma protein unbound and total concentration of acyclovir in horse plasma and body fluids: first, a liquid-liquid extraction was performed on plasma, combined with HPLC-fluorescence detection for the total plasma concentration; second a more sensitive method using high-performance liquid chromatography combined with heated electrospray ionization tandem mass spectrometry (LC-HESI-MS/MS) was described for plasma and for body fluids analysis. To obtain the unbound concentration of acyclovir in plasma, a simple deproteinization step using a Microcon filter was performed. Ganciclovir was used as an internal standard. Analysis was carried out on an Inertsil 5 ODS-3 column for the HPLC-fluorescence method. For the LC-HESI-MS/MS method a PLRP-S column was used. The limit of quantification (LOQ) for the total concentration was set at 50 and 2 ng mL(-1) for the HPLC-fluorescence method and the LC-HESI-MS/MS method, respectively. The limit of quantification for the unbound concentration was set at 5 ng mL(-1) and at 2 ng mL(-1) for body fluids. The methods were successfully used to perform pharmacokinetic and clinical studies in horses after intravenous and oral dosage of acyclovir and its prodrug valacyclovir.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of acyclovir in horse plasma and body fluids by high‐performance liquid chromatography combined with fluorescence detection and heated electrospray ionization tandem mass spectrometry

Maes

Garré

Desmet

et al. 2008

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Some of the analytical methods have been reported for acyclovir such as luorescence [18][19][20] direct UV [21][22][23][24] and HPLC-MS [25]. In that numerous HPLC methods have been reported for estimation of acyclovir in pharmaceutical formulations has been reported [26][27][28][29][30][31][32][33][34]. Among chromatographic techniques; the reversed-phase (RP) HPLC was widely used for the analysis.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of LC-MS/MS Method for the Analysis of P-Toluenesulfonicacid, In Antiviral Drug Acyclovir

Reddy¹

2015

IJIRSET

View full text Add to dashboard Cite

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC/MS/MS) method was developed and validated for the trace analysis (>1 ppm level) of p-Toluenesulfonicacid, a genotoxic impurity, in acyclovir drug substances. The chromatographic separation was achieved on Symmetry C-18 (150 X 4.6mm, 3.5µm) column using a mobile phase consisting of 5 mM ammonium acetate and methanol (70:30 v/v) at flow rate of 0.4 mL /min. The API-4000 LC/MS/MS was operated on an electrospray in negative mode. The selected ion monitoring (SIM) mode was used during the analytical run and it is able to quantitate up to 1.5 ppm of p-Toluenesulfonicacid. The newly developed method was validated as per achieved on International Conference on Harmonization (ICH) guidelines

show abstract

“…performance capillary electrophoresis [8], UV detection [9][10][11][12][13][14][15] and LC-MS/MS technique [16][17][18][19]. However, all these methods have their own disadvantages and which includes complicated sample pretreatment, time consuming chromatographic separation, low sensitivity and large volume of plasma (>500 µL).…”

mentioning

confidence: 99%

Role of RBC Partitioning and Whole Blood to Plasma Ratio in Bioanalysis: A Case Study With Valacyclovir and Acyclovir

Saha¹,

Kumar²,

Gurule³

et al. 2017

Mass Spectrom Purif Tech

View full text Add to dashboard Cite

A LC-MS/MS method was developed for simultaneous estimation of valacyclovir and acyclovir in human plasma. Plasma sample was extracted with solid phase extraction technique and chromatographic condition was set with Inertsil CN-3 (5 µm) column and mobile phase (1 mM ammonium acetate buffer -methanol, 50:50 v/v). Valacyclovir, acyclovir, Valacyclovir D4 and acyclovir D4 were detected in positive polarity in multiple reactions monitoring mode at mass transitions (m/z) 325.2→152.1, 226.2→152.1, 329.3→152.1 and 230.2→152.1, respectively. The validated calibration curve range for valacyclovir is 4.09 to 725.63 ng/mL and for acyclovir is 50.35 to 10017.29 ng/mL. During method development, stability of acyclovir in whole blood could not be established over the period for 2 hr as the K WB / P ratio for acyclovir is greater than 1 and although for valacyclovir it is less than 1. Therefore, the drug distribution of acyclovir was investigated in whole blood and plasma. Experimental data showed that an initial drop of acyclovir level in plasma due to the cellular uptake of acyclovir by erythrocytes. Hence, the spiked comparison samples were allowed to reach equilibrium (between RBC and plasma). After reaching the equilibration time (30.0 min), plasma was harvested from the spiked whole blood and processed as per the proposed protocol. From the blood stability data, we concluded that valacyclovir and acyclovir both are stable in blood for 2 hrs. The developed method was validated as per current regulatory guidelines and applied for valacyclovir and acyclovir bio-equivalence study.

show abstract

Determination of acyclovir in human serum by high-performance liquid chromatography using liquid–liquid extraction and its application in pharmacokinetic studies

Cited by 60 publications

References 22 publications

Determination of acyclovir in horse plasma and body fluids by high‐performance liquid chromatography combined with fluorescence detection and heated electrospray ionization tandem mass spectrometry

Determination of acyclovir in horse plasma and body fluids by high‐performance liquid chromatography combined with fluorescence detection and heated electrospray ionization tandem mass spectrometry

Development and Validation of LC-MS/MS Method for the Analysis of P-Toluenesulfonicacid, In Antiviral Drug Acyclovir

Role of RBC Partitioning and Whole Blood to Plasma Ratio in Bioanalysis: A Case Study With Valacyclovir and Acyclovir

Contact Info

Product

Resources

About