Determination of acylglycerols in vegetable oil methyl esters by on‐line normal phase LC‐GC

Lechner, Marion; Bauer-Plank, Christina; Lorbeer, Eberhard

doi:10.1002/jhrc.1240201103

Cited by 32 publications

(17 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biodiesel was also analyzed by GC for minor components such as sterols (14,15). Preseparation of biodiesel and its contaminants by high-performance liquid chromatography (HPLC) prior to GC was reported (16). However, other methods have been utilized in the analysis of biodiesel, including HPLC with density detection (17) or pulsed amperometric detection for determining glycerol (18), and viscosity (19).…”

mentioning

confidence: 99%

Rapid monitoring of transesterification and assessing biodiesel fuel quality by near‐infrared spectroscopy using a fiber‐optic probe

Knothe

1999

J Americ Oil Chem Soc

137

View full text Add to dashboard Cite

Vegetable oil esters, particularly methyl esters, are being explored and used as alternative diesel fuel (biodiesel). The transesterification reaction which yields the methyl esters can be monitored for completion by near-infrared (NIR) spectroscopy using a fiber-optic probe. Although the NIR method is less sensitive than gas chromatography (GC) for quantifying minor components, by correlation with existing GC or other analytical data, biodiesel fuel quality can be assessed through the NIR method. The NIR method is easier and faster to use than GC.Paper no. J9071 in JAOCS 76, 795-800 (July 1999).

show abstract

mentioning

confidence: 99%

Rapid monitoring of transesterification and assessing biodiesel fuel quality by near‐infrared spectroscopy using a fiber‐optic probe

Knothe

1999

J Americ Oil Chem Soc

137

View full text Add to dashboard Cite

show abstract

“…Many analytical methods have been used for the study of biodiesel transesterification (1). Among these, GC and LC-GC have been most widely used because of their accuracy in quantifying not only TG and FAME but also the intermediate reaction products, DG and MG (2,3). However, GC requires sample derivatization, which is time-consuming.…”

mentioning

confidence: 99%

A comparison of attenuated total reflectance‐FTIR spectroscopy and GPC for monitoring biodiesel production

Dubé

Zheng

McLean

et al. 2004

J Americ Oil Chem Soc

104

View full text Add to dashboard Cite

Gel permeation chromatography (GPC) and attenuated total reflectance (ATR)-FTIR spectroscopy were used to monitor the products of transesterification of waste frying oil in methanol to FAME or biodiesel. To evaluate the reliability and reproducibility of each method, quantitative analyses of mixtures of standards (TG, DG, MG, FAME, and glycerol) as well as lipid products of transesterification were carried out. The reproducibility of each method was found to be within ±1-5%. The differences between the results of the two methods were less than ±2%. The GPC method showed good separation of the intermediate products MG and glycerol from the TG starting material and FAME, but DG were not completely separated from TG. GPC gave good quantitative results for MG and FAME, but the TG and DG analyses required correction, depending on the mole ratio of TG/DG. In contrast, ATR-FTIR spectroscopy could only give quantitative data for the sum of TG + DG + MG.

show abstract

“…Several separation and analytical techniques such as gas chromatography (GC), thin-layer chromatography, highperformance liquid chromatography (HPLC), and infrared techniques [3,4,10] have been simplified, automated, and used to identify and measure fuel quality indices. According to Plattner [11] and other workers [12,13], the commonly used GC method for BD quantitation is not a convenient or direct analytical method for the detection and quantitation of the contaminants (triacylglycerols (TAG), diacylgylcerol (DAG), monoacylglycerol (MAG), free fatty acid (FFA), glycerol, and unreacted alcohol) in the products. They further indicated that the commonly used GC methods require carefully controlled sample derivatization (trimethylsilylation or acetylation) prior to the GC analysis [3,13,14].…”

Section: Introductionmentioning

confidence: 99%

“…According to Plattner [11] and other workers [12,13], the commonly used GC method for BD quantitation is not a convenient or direct analytical method for the detection and quantitation of the contaminants (triacylglycerols (TAG), diacylgylcerol (DAG), monoacylglycerol (MAG), free fatty acid (FFA), glycerol, and unreacted alcohol) in the products. They further indicated that the commonly used GC methods require carefully controlled sample derivatization (trimethylsilylation or acetylation) prior to the GC analysis [3,13,14]. In addition, an inert, short capillary column with stable and high column temperature (≥350°C) as well as elaborate pretreatment steps to separate the glycerol from the BD is usually required [11,15].…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantitation of Reaction Intermediates and Residuals in Lipase-Catalyzed Transesterified Oils by HPLC

Aryee

Phillip

Cue

et al. 2011

Appl Biochem Biotechnol

View full text Add to dashboard Cite

A high-performance liquid chromatography (HPLC) unit equipped with size exclusion column and a refractive index detector was used for simultaneous monitoring, identification, and quantitation of the reaction components from lipase-catalyzed transesterification of three oils. The procedure simultaneously separated and detected the unreacted triacylglycerols (TAG), diacyl-, and monoacyl-glycerol (DAG and MAG) co-products, residual alcohol as well as free fatty acid (FFA) based on retention times. The chromatograms showed well separated and resolved peaks. The elution of the components from the transesterification reaction in increasing order was: TAG < DAG < FFA < MAG. Generally, higher alcohol ratios decreased the conversion of TAG in all the oils studied with between 14% and 94% of TAG remaining at all the treatment combinations. Higher amount of salmon skin oil (SSO) TAG was generally converted to DAG than Rothsay composite (RC) and olive oil (OO) TAG. Relatively higher amount of OO DAG was converted to MAG than SSO and RC with only 5-14% DAG remaining in OO. RC and OO generally accumulated less MAG, and this was reflected as lower MAG levels in RC (<6%) and OO (<14%) compared with SSO (<27%). For the various treatment combinations and the three oils used in this study, the least amount of FFA was recorded in transesterified OO with a maximum of approximately 4%. This HPLC method can be used as a simple and fast technique to analyze the reaction components and products of transesterification reactions without the need for additional derivatization steps.

show abstract

Determination of acylglycerols in vegetable oil methyl esters by on‐line normal phase LC‐GC

Cited by 32 publications

References 19 publications

Rapid monitoring of transesterification and assessing biodiesel fuel quality by near‐infrared spectroscopy using a fiber‐optic probe

Rapid monitoring of transesterification and assessing biodiesel fuel quality by near‐infrared spectroscopy using a fiber‐optic probe

A comparison of attenuated total reflectance‐FTIR spectroscopy and GPC for monitoring biodiesel production

Identification and Quantitation of Reaction Intermediates and Residuals in Lipase-Catalyzed Transesterified Oils by HPLC

Contact Info

Product

Resources

About