Determination of Aflatoxin M1 in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay

Atanasova, Milka; Vasileva, N.; Godjevargova, Tzonka

doi:10.1080/00032719.2016.1187626

Cited by 15 publications

(3 citation statements)

References 26 publications

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“…The precision of the mpEIA assay was estimated based on repeat measurements of standard diluted urine samples with spiked concentrations of gB-HCMV ( Table 3). The relative standard deviations have small values, lesser than about 6.5% in all cases (n=3), in accordance with the usually obtained for these types of immunosensors based on magnetic particles compared with classical ELISAs [17,36]. The relative errors at these…”

Section: Immunosensor Analytical Performancesupporting

confidence: 88%

A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification

Pires

Arcos‐Martínez

Dias-Cabral

et al. 2018

Journal of Pharmaceutical and Biomedical Analysis

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Human cytomegalovirus (HCMV) is a herpes virus that can cause severe infections. Still, the available methods for its diagnostic have the main disadvantage of requiring long time to be performed. In this work, a simple magnetic particle-based enzyme immunoassay (mpEIA) for the quantification of glycoprotein B of Human cytomegalovirus (gB-HCMV) in urine samples is proposed. The immunosensor scheme is based on the analyte protein gB-HCMV sandwiched between a primary monoclonal antibody, (MBs-PrG-mAb1), and a secondary anti-gB-HCMV antibody labelled with Horseradish peroxidase (Ab2-HRP) to allow spectrophotometric detection. The mpEIA analytical performance was tested in urine samples, showing a linear dependence between gB-HCMV concentration and the absorbance signal at 450 nm in a range of concentrations from 90 to 700 pg mL. The calculated detection limits for gB-HCMV were 90 ± 2 pg mL and the RSD was about 6.7% in urine samples. The immunosensor showed good selectivity against other viruses from Herpesviridae family, namely varicella zoster and Epstein Barr viruses. The recoveries of spiked human urine samples at 0.30-0.50 ng mL concentration levels of gB-HCMV ranged between 91 to 105%. The proposed mpEIA method was validated following the guidelines of the European Medicines Agency (EMEA-2014), and allows rapid, successful and easy quantification of gB-HCMV in urine samples.

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Section: Immunosensor Analytical Performancesupporting

confidence: 88%

A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification

Pires

Arcos‐Martínez

Dias-Cabral

et al. 2018

Journal of Pharmaceutical and Biomedical Analysis

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“…The immunosensor had a detection limit of 5.5 pg/mL and a linear assay range of 0.01–100 ng/mL. Magnetic beads were also used in the work by Atanasova and colleagues concerning the detection of aflatoxin M1 [ 94 ]. The magnetic nanoparticle-based fluorescent immunoassay provided a limit of detection for aflatoxin M1 2.9 pg/mL and a linear calibration range of 3.0 to 100 pg/mL.…”

Section: Progress On Immunosensors For Toxic Biological Warfare Agent...mentioning

confidence: 99%

Immunosensors for Assay of Toxic Biological Warfare Agents

Pohanka

2023

Biosensors

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An immunosensor for the assay of toxic biological warfare agents is a biosensor suitable for detecting hazardous substances such as aflatoxin, botulinum toxin, ricin, Shiga toxin, and others. The application of immunosensors is used in outdoor assays, point-of-care tests, as a spare method for more expensive devices, and even in the laboratory as a standard analytical method. Some immunosensors, such as automated flow-through analyzers or lateral flow tests, have been successfully commercialized as tools for toxins assay, but the research is ongoing. New devices are being developed, and the use of advanced materials and assay techniques make immunosensors highly competitive analytical devices in the field of toxic biological warfare agents assay. This review summarizes facts about current applications and new trends of immunosensors regarding recent papers in this area.

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“…The excess of the conjugate is directly proportional to the OPP concentration in the sample. On this principle have been developed assays for the detection of ochratoxin, aflatoxin M1, enterotoxin A, and antibiotics in milk [38][39][40][41]. There are few publications about MNP-based immunoassay for the determination of phosphorus pesticides in milk [42][43][44].…”

Section: Fpia and Mnps Based Ifamentioning

confidence: 99%

Immunochemical Assays for Determination of Organophosphorus Pesticides in Milk

Godjevargova¹

2019

JDVS

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The detection of pesticide residues is an important task in ensuring the safety of milk. In the last decade, organophosphorus insecticides have been used, but they also pose a serious risk because of their high toxicity. For fast screening of milk, immunochemical methods of analysis are appropriate. Enzyme-linked immunosorbent assay is the most common assay mode and base of many commercialized assay kits. Residues of some organophosphorus compounds in milk and dairy products determined by ELISA are shown. For fast screening of pesticides, the lateral flow strips are also described. The advantages of a new developed method MNPs-based immunoassay for determination of phosphorus pesticides in milk was described and some publications were represented. In recent years, simultaneous identification of more than one pesticide is increasingly perceptual, that way the examples for multi-immunoassays for determination of organophosphorus pesticides was presented.

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Determination of Aflatoxin M1 in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay

Cited by 15 publications

References 26 publications

A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification

A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification

Immunosensors for Assay of Toxic Biological Warfare Agents

Immunochemical Assays for Determination of Organophosphorus Pesticides in Milk

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