Determination of aggregation thresholds of UV absorbing anionic surfactants by frontal analysis continuous capillary electrophoresis

Saux, Thomas Le; Varenne, Anne; Gareil, Pierre

doi:10.1016/j.chroma.2004.03.006

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…The free fraction of 0.42 under plateau peak conditions is in agreement with the previously determined value of 0.39 [16]. The degree of complexation of 2-propylisochinolinium bromide with dextran sulfate was also studied in the frontal analysis continuous CE [21,22] mode and results similar to those obtained by CE-FA were found (Table 2). In addition, results obtained by ED were in good agreement with those achieved under plateau peak conditions (Table 2), lending support to the validity of the CE-FA method.…”

Section: Dextran Sulfate Interactionssupporting

confidence: 75%

Pre-equilibrium capillary zone electrophoresis or frontal analysis: Advantages of plateau peak conditions in affinity capillary electrophoresis

et al. 2005

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The feasibility of using the affinity CE methodologies pre-equilibrium CZE and CE frontal analysis was tested on interaction systems exhibiting rapid on-and-off kinetics. Experimentally, the methodologies differ only with respect to the volume of sample introduced into the capillary. Pre-equilibrium CZE has been considered amendable to interactions with slow on-and-off kinetics only; however, it has recently been applied in studies of interactions with fast on-and-off kinetics. The effect of varying the sample volume introduced hydrodynamically into the capillary on the apparent degree of complexation was studied. For two different binding systems, the fraction of free analyte was found to be overestimated using pre-equilibrium CZE as compared to volumes providing plateau peak conditions as used with frontal analysis. Results indicate that frontal analysis conditions lead to more robust binding assays and thus more reliable data. The validity of data obtained by pre-equilibrium CZE may be low, thus the use of an experimental setup providing plateau peaks is highly recommended. It is suggested that the effect of altering the sample volume on the degree of binding should be investigated as part of method development and validation.

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Section: Dextran Sulfate Interactionssupporting

confidence: 75%

Pre-equilibrium capillary zone electrophoresis or frontal analysis: Advantages of plateau peak conditions in affinity capillary electrophoresis

et al. 2005

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“…The measured CMC values are in good accordance with literature data [8,24] although a direct comparison is difficult. It is well known that CMC values are dependent on temperature and, particularly in the case of charged surfactants, ionic strength and counterions [8].…”

Section: Comparison Of Ce-fa and Conductometry For Determining Cmcssupporting

confidence: 85%

“…If the experimental conditions are chosen correctly, the height of the plateau or plateau peak corresponding to the ligand is directly proportional to the concentration of free ligand in the original sample. Recently, a new method for determining CMC values of UV-absorbing anionic surfactants based on FA was introduced [24]. This method is attractive as it does not rely on marker compounds and appears to be quite accurate and easily implemented.…”

Section: Introductionmentioning

confidence: 99%

CE frontal analysis employing contactless conductivity detection for determination of CMCs of non‐UV absorbing charged surfactants

2007

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Micellar systems composed of surfactants are used extensively in academia and industry for many different applications. In this work a highly versatile CE method for determination of CMCs of charged surfactants has been developed. In the case of positively charged surfactants a coating procedure of the fused-silica capillary was used, whereas negatively charged surfactants were analyzed using uncoated capillaries. The CE method is based on frontal analysis (FA) employing use of contactless conductivity and UV detection. The main advantages of the method are that it can be used for non-UV absorbing surfactants without introducing marker compounds which previously has been found to affect CMCs, requires very limited sample volume and is easily implemented and automated using standard CE equipment. The fact that counterions and different aggregated states are separated allows a detailed characterization of the micelle systems using the developed method. In the case of UV absorbing surfactants similar results were obtained employing contactless conductivity and UV detection. Finally, CMCs obtained using conductometry gave similar results as compared to the developed CE-FA procedure.

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The Concept of Stationary and Moving Boundaries Modelled as Accelerating or Decelerating Planes in the Understanding of Sweeping Processes Employed for Online Focusing in Capillary Zone Electrophoresis and Electrokinetic Chromatography

2017

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Determination of aggregation thresholds of UV absorbing anionic surfactants by frontal analysis continuous capillary electrophoresis

Cited by 6 publications

References 21 publications

Pre-equilibrium capillary zone electrophoresis or frontal analysis: Advantages of plateau peak conditions in affinity capillary electrophoresis

Pre-equilibrium capillary zone electrophoresis or frontal analysis: Advantages of plateau peak conditions in affinity capillary electrophoresis

CE frontal analysis employing contactless conductivity detection for determination of CMCs of non‐UV absorbing charged surfactants

The Concept of Stationary and Moving Boundaries Modelled as Accelerating or Decelerating Planes in the Understanding of Sweeping Processes Employed for Online Focusing in Capillary Zone Electrophoresis and Electrokinetic Chromatography

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