Determination of all-trans retinoic acid loaded in solid lipid nanoparticles by differential pulse voltammetry at glassy carbon electrode

Silva, Flávia Lidiane Oliveira da; Tinoco, Letícia Márcia da Silva; Ferreira, Lucas Antônio Miranda; Malagutti, Andréa R.; Carneiro, Guilherme

doi:10.1016/j.electacta.2015.10.018

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…6 ). These findings are consistent with previous electrochemical studies of retinoids ( 28 , 31 ), especially of all- trans -retinoic acid ( 32 ). Oddly enough, when the potential range was extended to +1.6 V, other two oxidation processes appeared ( Fig.…”

Section: Resultssupporting

confidence: 93%

Electrochemical Study and Determination of All-trans Retinol at Carbon Paste Electrode Modified by Surfactant

Žabčíková

Mikýsek

Červenka

et al. 2018

Food Technol. Biotechnol.

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SummaryThe oxidation mechanism of all-trans-retinol (vitamin A1) and its several esters in non-aqueous, aqueous organic mixture, and pure aqueous media was investigated by cyclic voltammetry. The oxidation occurred in several irreversible steps. The calculated highest density of electrons in retinoid molecules which are delocalized over carbon atoms of the five conjugated double bonds (C5-C14) was found in the part of the molecule involved in oxidation processes. The most sensitive oxidation peak (at +0.8 V vs. Ag/AgCl) was used for development of new direct voltammetric method based on differential pulse voltammetry for the determination of retinol at carbon paste electrode modified with surfactant sodium dodecyl sulfate (CPE/SDS). The results show that 30% (by mass) of modifier SDS exhibited optimal sensitivity and shape of voltammograms. Compared to commonly used glassy carbon electrode (GCE), the CPE/SDS showed significant progress in the retinol electroanalysis. The linear ranges for retinol determination were 1.5·10−6–1.8·10−4 M for CPE/SDS and 4.4·10−6–7.0·10−4 M for GCE with the detection limits of 1.3·10−6 and 4.6·10−7 M, respectively.

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Section: Resultssupporting

confidence: 93%

Electrochemical Study and Determination of All-trans Retinol at Carbon Paste Electrode Modified by Surfactant

Žabčíková

Mikýsek

Červenka

et al. 2018

Food Technol. Biotechnol.

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“…As expected, high EE (96.7 ± 4.9%) was obtained, which is in good agreement with our previous work involving incorporation of HIP between ATRA and lipophilic amines in lipid nanocarriers. ATRA concentration in the AP was found to be negligible as previously reported (Carneiro et al, 2012;Da Silva et al, 2015;Silva et al, 2015;Silva et al, 2016). The high ATRA retention in lipid matrix is related to the hydrophobicity of the counterion used in the HIP strategy, while the lipophilic amine is associated with intense interaction of ATRA with the lipid matrix (Oliveira et al, 2017).…”

Section: Development Of Atra-ne Formulationssupporting

confidence: 73%

Hyaluronic acid-coated nanoemulsions loaded with a hydrophobic ion pair of all-trans retinoic acid for improving the anticancer activity

Tinoco

Silva

Ferreira

et al. 2018

Braz. J. Pharm. Sci.

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All-trans retinoic acid (ATRA) has been studied for the treatment of cancer, including leukemia and breast cancer. This work aims to develop nanoemulsions (NE) loaded with a hydrophobic ion pair (HIP) of all-trans retinoic acid (ATRA) and a lipophilic amine, stearylamine (SA), and coated with hyaluronic acid (HA) to enhance anticancer activity and reducing toxicity. Blank NE was prepared by spontaneous emulsification and optimized prior to HIP incorporation. NE-ATRA was electrostatically coated with different concentrations of HA. Incorporation of ATRA-SA led to monodisperse NE with small size (129 ± 2 nm; IP 0.18 ± 0.005) and positive zeta potential (35.7 ± 1.0 mV). After coating with 0.5 mg/mL HA solution, the mean diameter slightly increased to 158 ± 5 nm and zeta potential became negative (-19.7 ± 1.2 mV). As expected, high encapsulation efficiency (near 100%) was obtained, confirmed by polarized light microscopy and infrared analysis. Formulations remained stable over 60 days and release of ATRA from NE was delayed after the hydrophilic HA-coating. HA-coated NE-ATRA was more cytotoxic than free ATRA for MDA-MB-231 and MCF-7 breast cancer cell lines, especially in the CD44 overexpressing cells. Blank coated formulations showed no cytotoxicity. These findings suggest that this easily-made HA-coated NE-ATRA formulation is a promising alternative for parenteral administration, thus improving the breast cancer therapy with this drug.

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“…In previous studies, it was found that α-tocopherol provides only one oxidation peak in a non-aqueous solution (Sýs et al, 2016). On the other hand, retinol acetate provides three oxidation steps under the same conditions (da Silva et al, 2015). Fortunately, potential values of these three oxidation peaks of the retinol acetate are sufficiently distant from the potential value of α-tocopherol peak.…”

Section: Introductionmentioning

confidence: 94%

Square wave voltammetry at carbon paste electrode modified with surfactant for alpha tocopheryl acetate determination in cosmetics

Žabčíková

Nallbani

Sýs

et al. 2018

Potr. S. J. F. Sci.

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The aim of this study was describe electrochemical properties of a carbon paste electrode (CPE) bulk modified with 30% (w/w) surfactant sodium dodecyl sulphate (CPE/SDS) and demonstrates its application in the determination of α-tocopheryl acetate (α-TAc), known as vitamin E acetate, in selected cosmetic products, especially body creams. In addition to anionic SDS, cationic hexadecylpyridinium chloride monohydrate (CPC) was also tested as possible modifier. It was found that selection of surfactant type and its content significantly affect an electrical conductivity and mechanical stability of these heterogeneous electroanalytical sensors in pure organic solvents. Under this study, it was found that CPC is a totally inappropriate mediator due to very high backgroundcurrent. Together with other lipophilic vitamins characterized by antioxidant activity (dominantly retionoids), this completely synthetic substance is widely used as significant cosmetic additive due its preservative properties. Monitoring of its content in cosmetic products is usually performed by high-performance liquid chromatography (HPLC) with UV detection. This standard analytical protocol is always burdened with the complex and time-consuming preparation of the sample before analysis. For that reason, robust and simple electroanalytical method based on anodic oxidation of the α-TAc at CPE/SDS by square wave voltammetry (SWV) performed in pure organic electrolyte (99.8% acetonitrile containing 0.1 mol•L -1 LiClO 4 ) was developed. Moreover, simple dissolution of sample in supporting electrolyte using ultrasonic bath and subsequent filtering through a stacked filter included all the necessary procedures for sample preparation. The linear range from 0.1 to 1.2 mmol•L -1 and limit of detection 37 µmol•L -1 were found at pulse amplitude 10 mV and frequency 10 Hz as optimum. In analysis of selected cosmetics, the developed electroanalytical method was not validated using comparison with standard HPLC. At least, the recovery was verified by analysis of model sample and value 95.8% was calculated.

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Determination of all-trans retinoic acid loaded in solid lipid nanoparticles by differential pulse voltammetry at glassy carbon electrode

Cited by 7 publications

References 28 publications

Electrochemical Study and Determination of All-trans Retinol at Carbon Paste Electrode Modified by Surfactant

Electrochemical Study and Determination of All-trans Retinol at Carbon Paste Electrode Modified by Surfactant

Hyaluronic acid-coated nanoemulsions loaded with a hydrophobic ion pair of all-trans retinoic acid for improving the anticancer activity

Square wave voltammetry at carbon paste electrode modified with surfactant for alpha tocopheryl acetate determination in cosmetics

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