Determination of allele frequencies of growth hormone AluI polymorphism in Brown Swiss, Holstein, native East Anatolian Red and Turkish Grey breeds

Abstract

A total of 177 cattle of four breeds were genotyped for the bovine growth hormone (BGH)‐AluI polymorphism by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP). The genotype and gene frequencies for each breed were determined and tested to be in Hardy–Weinberg equilibrium. According to breeds, frequencies of allele L gene were 0.905 for Brown Swiss, 0.898 for Holstein, 0.976 for East Anatolian Red and 0.893 for Turkish Grey Breeds. The allele L was predominant and variant VV was not detected in the breeds studied. BGH‐AluI genotypes were found to be in equilibrium within and among breeds.

bGH/AluI and CSN3/HinfI Gene Polymorphisms in Holstein Cattle

“…In the analysis, 245-bp DNA region was amplified using primers growth hormone (bGH) gene region, Forward: 5'-GTA GGG GAG GGT GGA AAA TG -3, Revers:5' TGA CCCTCA GGT ACG TCT CC -3, designed by Ozdemir [4] and 351 bp DNA region was amplified using primers Kappa-Casein (CSN3) gene region, Forward:5'-ATT TAT GGC CAT TCC ACC AA-3', Revers:5'-ATT AGC CCA TTT CGC CTT CT-3'. PCR amplification conditions were set (the initial denaturation temperature would be the same) as 1 cycle/ 5 minutes at 94 0 C for bGH and CSN3; after the 2nd denaturation had been set as 30 cycles, elongation cycles were set 45 seconds at 94 °C, 58°C and 72°C for bGH, and 45 seconds at 94 °C, 60°C and 72°C for CSN3 and final extension temperatures were set 1 cycle/7 minutes at 72°C.…”