Determination of Alpha-Amino Nitrogen as Ammonia

Schlenker, Frank S.

doi:10.1021/ac60007a014

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…The two imino acids react better with ninhydrin under acidic conditions (25,26). The presence of an acid reduces the blank color because amino acids and ammonia do not give a color with ninhydrin under acidic conditions (24). Because there is essentially no proline lost in locating it on the paper (Table II, 2, 3), no interference by ammonia, and no fading of color in the dark in 24 hours, highly reproducible determinations can be made.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of an acid reduces the blank color because amino acids and ammonia do not give a color with ninhydrin under acidic conditions (24). Because there is essentially no proline lost in locating it on the paper (Table II, 2, 3), no interference by ammonia, and no fading of color in the dark in 24 hours, highly reproducible determinations can be made. The final color obtained from the other acids is not reduced by the color developed in locating proline and asparagine.…”

Section: Discussionmentioning

confidence: 99%

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Determination of Amino Acids from Plants by Paper Chromatography

Thompson¹,

Morris²

1959

Anal. Chem.

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potassium was found in the basic amino acid fraction and no detectable amount with the other amino acids. In obtaining the amine fraction, the monovalent cations are removed from the resin with 0.5V hydrochloric acid, but the diand trivalent cations are only partially eluted. The remaining polyvalent cations which are eluted with 6Ar hydrochloric acid usually do not interfere with the chromatography of amines.The acid form of Dowex 50 does not retain strongly acidic amino acids such as cysteic acid and taurine (3) and these are not purified. Such strongly acidic amino acids have not been reported in plants (20). It would be possible to retain these acids and other anions on the salt form of a strongly basic resin while nonionic substances would pass through. Such variations would provide a basis for a more extensive fractionation scheme whereby separate fractions with nonionic materials, anions, neutral amino acids, acidic amino acids, basic amino acids, cations, and some amines could be obtained (10, 17).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Determination of Amino Acids from Plants by Paper Chromatography

Thompson¹,

Morris²

1959

Anal. Chem.

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Absorption of Amino Acids by Yeasts From a Semi-Defined Medium Simulating Wort

Jones¹,

Pragnell²,

Pierce³

1969

Journal of the Institute of Brewing

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The biosynthetic pathways involved when a-amino acids are absorbed by yeasts from a semi-defined medium simulating wort have been measured using 15N and "C isotopes. When grown in this medium under brewery conditions, a complex transaminase equilibrium system operates within the cell, showing that the theory of intact assimilation of amtno acids into yeast protein is invalid.A relatively high level of specificity was found in respect of the transfer of carbon skeletons of amino acids in the medium to carbon skeletons of yeast protein amino acids and the contributions in respect of each amino acid have been measured through out growth.Simple sugars have been shown to contribute significantly to the carbon skeletons of most amino acids found in yeast and the extent of these syntheses has been quanti tatively assessed.

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Photochemistry of proteins. XVII. Inactivation of enzymes with ultraviolet light and photolysis of the peptide bond

McLaren¹,

Gentile²,

Kirk³

et al. 1953

J. Polym. Sci.

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Ribonuclease and carboxypeptidase are inactivated by ultraviolet light (2537 A.) with quantum yields of 0.03 and 0.001–0.005 respectively. The quantum yields for low molecular weight proteins are of the order of 0.03 and are higher than those for splitting peptide bonds in typical peptides (0.004–0.0004 or less). It is postulated that the primary process of inactivation involves modification of aromatic residues in proteins. A possible reason for the inverse relationship between quantum yields and molecular weights of enzymes and proteins is discussed.

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Determination of Alpha-Amino Nitrogen as Ammonia

Abstract: When subjected to alkaline aeration at pH 11.0, the complex is hydrolyzed and the liberated ammonia so isolated is a measure of the amount of a-amino nitrogen that was originally present in the solution.

Cited by 7 publications

References 17 publications

Determination of Amino Acids from Plants by Paper Chromatography

Determination of Amino Acids from Plants by Paper Chromatography

Absorption of Amino Acids by Yeasts From a Semi-Defined Medium Simulating Wort

Photochemistry of proteins. XVII. Inactivation of enzymes with ultraviolet light and photolysis of the peptide bond

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