Determination of Ambroxol Hydrochloride by HPLC

Macrocycleversus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes

“…2.91, 3.24 and 6.40 for clenbuterol, ambroxol and bromhexine, respectively, and required a different chromatographic separation approach. Application of previously reported RP-LC systems for determination of ambroxol and bromhexine [18][19][20][21][22][23][24][25]43] were excluded from consideration as they used acidic mobile phases with addition of triethylamine, nonylamine [18,[20][21][22] or ammonium acetate and diammonium phosphate [20,22,43] as "silanol blockers". The use of such mobile phase additives forming highly charged "onium" cations would lead to significantly decreased sensitivity and selectivity of potentiometric detection by enhancing background noise [35,36,41] during determination of the current cationic mucolytic agents.…”

Colorimetric and Atomic Absorption Spectrometric Determination of Mucolytic Drug Ambroxol Through Ion-Pair Formation with Iron and Thiocyanate

“…Bearing in mind that a stationary phase with lower hydrophobicity and silanol activity reduces the interaction leading to tailing in peaks for basic ionizable analytes [56], C-8 analytical column was chosen in the present study instead of C-18 column used in [5]. Another subject is the optimum pH of buffer employed in the mobile phase.…”

“…For this reason, the reversed-phase HPLC method was employed as a referee method for the proposed methods. The procedure was modified from the reported method [5].…”

“…In aqueous solutions of pH 6.1, AMB is completely ionized, since its pK a value is around 8.1 (benzylamino group) [3]. To date, several analytical methods for the determination of AMB are described, including high-performance liquid chromatography [4][5][6][7][8][9], UV-visible spectrophotometry [10,11], flow injection analysis [12][13][14][15], gas chromatography [16,17], capillary electrophoresis [18,19], Raman spectroscopy [20,21], potentiometry [22], and voltammetry [23][24][25].…”

Validated derivative and ratio derivative spectrophotometric methods for the simultaneous determination of levocetirizine dihydrochloride and ambroxol hydrochloride in pharmaceutical dosage form

“…Some analytical methods have been reported for the determination of LCD either alone [6][7][8][9] or in combination with other drugs using high performance liquid chromatography [10][11][12][13][14][15], thin layer chromatography [16] and UV spectrophotometry [17][18][19][20][21][22]. As well, a number of methods have been reported for the individual determination of ABH [23][24][25][26][27], and in combination with other drugs in pharmaceutical dosage form by high performance liquid chromatography [28][29][30][31][32][33], liquid chromatography-mass spectroscopy/mass spectroscopy [34], thin layer chromatography [35], UV spectrophotometry [36,37] and high performance thin layer chromatography [38,39].…”