Determination of ambroxol in syrups using diffuse reflectance spectroscopy

Abstract

Recebido em 6/8/08; aceito em 19/1/09; publicado na web em 3/7/09 This paper reports an analytical method for the determination of ambroxol in micellar medium by spot test-diffuse reflectance spectroscopy. The reflectance measurements were performed analyzing the colored compound (λ= 520 nm) produced from the reaction between ambroxol and p-dimethylaminocinnamaldehyde on the surface filter paper. The linear range was from 1.21 × 10 −3 to 9.65 × 10 −3 mol L -1 (500 -4000 µg mL -1). The limit of detection and quantification were 3.50 x 10 -4 mol L -1 (145 µg mL -1 ) and 1.16 x 10 -3 mol L -1 (481 µg mL -1 ), respectively. Five commercial samples were analysed and the results obtained by the proposed method were in good agreement with those obtained by the literature method at 95% confidence level.

Validated derivative and ratio derivative spectrophotometric methods for the simultaneous determination of levocetirizine dihydrochloride and ambroxol hydrochloride in pharmaceutical dosage form

“…Some analytical methods have been reported for the determination of LCD either alone [6][7][8][9] or in combination with other drugs using high performance liquid chromatography [10][11][12][13][14][15], thin layer chromatography [16] and UV spectrophotometry [17][18][19][20][21][22]. As well, a number of methods have been reported for the individual determination of ABH [23][24][25][26][27], and in combination with other drugs in pharmaceutical dosage form by high performance liquid chromatography [28][29][30][31][32][33], liquid chromatography-mass spectroscopy/mass spectroscopy [34], thin layer chromatography [35], UV spectrophotometry [36,37] and high performance thin layer chromatography [38,39].…”

Development and Validation of Absorbance Correction Method for Simultaneous Determination of Levocetirizine and Ambroxol in Combined Tablet Dosage Form

“…The above three pharmacopoeias describe potentiometry method for its estimation [7][8][9] . Literature survey reveals that diffuse reflectance spectroscopy 10 , spectrophotometry 11,12 , RP-HPLC 13 , stability indicating HPTLC 14 and acid dye methods 15 The combination of Levocetirizine and Ambroxol is not official in any pharmacopoeia; hence no official method is available for estimation of these two drugs in combined dosage form. Literature survey reveals that several RP-HPLC [16][17][18] and spectrophotometric 19 methods have been reported for estimation of Levocetirizine and Ambroxol in combined dosage form.…”