Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization

Wong, Lawrence T.; Beaubien, A R; Pakuts, Attila

doi:10.1016/s0378-4347(00)80518-6

Cited by 42 publications

(21 citation statements)

References 13 publications

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“…(192) Nitrobenzene derivatives of neomycin B and C can be separated by normal-phase HPLC and detected at 350 nm. (193) Similar methods using 1-fluoro-2,4-dinitrobenzene to form 2,4-dinitrophenyl derivatives of neomycin B and C (194,195) and amikacin (196,197) have also been published. Neomycin and other aminoglycosides have been converted to benzoyl derivatives before UV detection at 230 nm.…”

Section: Antibioticsmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

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This article describes both pre‐ and postcolumn derivatization chemistry used in conjunction with either chromatography or capillary electrophoresis (CE) to facilitate the determination of drugs. Generally, only prederivatization is used in gas chromatography (GC), principally to enhance the volatility, temperature stability, and/or detectability. The GC section considers derivatization of drugs by reagent class: alkylation, acylation, and silylation. The GC sample‐handling section describes an approach that combines the extraction and derivatization steps together. Both pre‐ and postcolumn derivatization are common approaches for high‐performance liquid chromatography (HPLC) and many of these methods have been adapted for CE. Derivatization for HPLC is often directed toward aliphatic amines, carboxylic acids, or alcohols that are difficult to detect at low levels by absorbance, luminescence, or electrochemical means. In addition, small hydrophilic molecules upon prederivatization are often converted into larger more hydrophobic compounds, making reversed‐phase HPLC easier or even feasible. The HPLC section considers derivatization of drugs based on the following types: alkaloids, amines, antibiotics, barbiturates, carbonyl compounds and carboxylic acids, catecholamines, hydroxy compounds, steroids, and sulfur compounds. For the GC and HPLC sections, tables are included, giving the structures of the more important derivatizing agents, the analytes, and the corresponding reaction products. A brief rationale for derivatization chemistry with CE concludes this article. For CE, derivatization is often done to improve detectability as the path length for absorbance detection is very short and fluorescence can be effective using laser‐based systems.

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Section: Antibioticsmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

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“…Amikacin sulfate is quantitatively determined through various analytical techniques such as spectrophotometry, 4-8 spectroflourimetry 9-13 and HPLC methods. [14][15][16][17][18][19][20][21][22][23][24][25] The aminoglycoside antibiotic activity can be increased by association with Herbs like activity by Pityrogramma calomelanos (L.). 26 The spectroscopic method for assay of amikacin sulfate is not official in any pharmacopoeia.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of amikacin Sulfate using vanillin from pure and commercial brands available in Pakistan

Mughal¹,

Dayo²,

Ghoto³

et al. 2015

J YOUNG PHARM

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Objective: To determine the amikacin sulfate by developing a new spectrophotometric method by derivatization using double beam spectrophotometer from pure and commercial brands. Method: A quantitative analytical method was developed by amikacin sulfate using spectrophotometer after derivatization with vanillin under optimized parameters like pH, heating time and temperature, volume of reagent, Impact of mixing order, Effect of addition of solvents and Effect of excipients. The method was successfully applied on bulk and different brands containing amikacin sulfate. Results: The bulk and pharmaceutical analysis was carried out of different brands with formation of slight yellow colored imine base. The product showed absorbance at 400 nm w i t h molar absorptivity of 5.

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“…influence by various experimental parameters, incompleteness of derivatization reaction, appearance of additional peaks due to unreacted reagent, use of salt laden mobile phases, prolonged analysis time, additional cost for derivatization system and reagents). Especially for amikacin determination, it has been reported that precolumn derivatization with o-phthalaldehyde or 1-fluoro-2,4-dinitrobenzene results in unstable derivatives [11][12][13]. Additionally, the LC-UV-vis methods are not suitable for the simultaneous determination of sulfates in raw materials and therefore an extra titrimetric procedure has to be performed.…”

Section: Introductionmentioning

confidence: 99%