Determination of amino acids in food and feed by microwave hydrolysis and UHPLC-MS/MS

Weber, Patrick

doi:10.1016/j.jchromb.2022.123429

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…Previous literature has reported the appropriate microwave conditions that were used for milk powder digestion by the same manufacturer of the digestion apparatus [ 30 ]. Meanwhile, we justified the upper and lower limits of these conditions by referring to the relevant literature reports that were used for natural product digestion [ 16 , 31 ]. Finally, the level of influencing factors were determined, including microwave heating temperature (150, 165, and 180 °C), microwave heating time (10, 20, and 30 min), and solid–liquid ratio (25, 50, and 70 g/mL).…”

Section: Methodsmentioning

confidence: 99%

“…Domestic standards for the determination of amino acid content in natural product samples often refer to the National Standard for Food Safety of the Determination of Amino Acids in Food (GB 5009.124-2016). Foreign official standards mainly refer to the International Organization for Standardization’s (ISO) 13904:2016, the American Association of Analytical Chemists’ (AOAC) 994.12 (1997), and the European Commission (EC) 152/2009 [ 16 ]. The above methods stipulate that the sample for determination of free amino acids in proteins should be hydrolyzed by hydrochloric acid (6 mol/L) at 110 °C for 20–24 h. In recent years, the microwave digestion method has been gradually used in the pretreatment for the determination of amino acids in proteins with the advantages of accelerating the rate of hydrolysis, simple processing, and reliable results.…”

Section: Introductionmentioning

confidence: 99%

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An Optimized Microwave-Assisted Digestion Method to Analyze the Amino Acids Profile of Quisqualis Fructus from Different Planted Origins

Dai,

Yang,

Wang

et al. 2024

Foods

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This study aims to establish a rapid and convenient microwave-assisted digestion method for sample pretreatment to determine amino acid profiles in natural products. This method was applied to analyze the amino acid profiles of Quisqualis Fructus (QF) from different planted origins. The microwave-assisted digestion conditions were optimized by a response surface methodology (RSM), and 17 amino acids in different planted origins of QF were determined by an automatic amino acid analyzer according to the optimized digestion conditions. The contents of 17 amino acids in QF from different planted origins were further analyzed by fingerprint and chemometric analysis. The temperature of microwave digestion at 167 °C, time of microwave digestion at 24 min, and a solid–liquid ratio of 46.5 g/mL was selected as the optimal digestion conditions. The total content of 17 amino acids in QF from different planted origins ranged from 71.88 to 91.03 mg/g. Amino acid composition and nutritional evaluation indicated that the content of medicinal amino acids was higher than aromatic amino acids. The results of fingerprint analysis reflected that the similarity between the 16 batches of QF ranged from 0.889 to 0.999, while chemometrics analysis indicated amino acid content in QF varied from different planted origins, and six important differential amino acids were screened. Compared with the traditional extraction method, microwave-assisted digestion with response surface optimized has the advantages of rapidity, convenience, and reliability, which could be used to study the amino acid profiles in natural products. The amino acid profile of QF indicated that it has a rich medicinal nutritional value. Different planted origins of QF have a high degree of similarity and could be effectively distinguished by chemometric analysis.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Optimized Microwave-Assisted Digestion Method to Analyze the Amino Acids Profile of Quisqualis Fructus from Different Planted Origins

Dai,

Yang,

Wang

et al. 2024

Foods

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“…The supernatant was aspirated, diluted with pure water, and vortexed for 5 min. The diluted samples were centrifuged at 4 °C for 5 min at 12,000 rpm; 50 μl of supernatant was taken for analysis [ 58 ].…”

Section: Methodsmentioning

confidence: 99%

Eubacterium rectale Improves the Efficacy of Anti-PD1 Immunotherapy in Melanoma via l -Serine-Mediated NK Cell Activation

et al. 2023

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Natural killer (NK) cells, as key immune cells, play essential roles in tumor cell immune escape and immunotherapy. Accumulating evidence has demonstrated that the gut microbiota community affects the efficacy of anti-PD1 immunotherapy and that remodeling the gut microbiota is a promising strategy to enhance anti-PD1 immunotherapy responsiveness in advanced melanoma patients; however, the details of the mechanism remain elusive. In this study, we found that Eubacterium rectale was significantly enriched in melanoma patients who responded to anti-PD1 immunotherapy and that a high E. rectale abundance was related to longer survival in melanoma patients. Furthermore, administration of E. rectale remarkably improved the efficacy of anti-PD1 therapy and increased the overall survival of tumor-bearing mice; moreover, application of E. rectale led to a significant accumulation of NK cells in the tumor microenvironment. Interestingly, conditioned medium isolated from an E. rectale culture system dramatically enhanced NK cell function. Gas chromatography–mass spectrometry/ultrahigh performance liquid chromatography–tandem mass spectrometry-based metabolomic analysis showed that l -serine production was significantly decreased in the E. rectale group; moreover, administration of an l -serine synthesis inhibitor dramatically increased NK cell activation, which enhanced anti-PD1 immunotherapy effects. Mechanistically, supplementation with l -serine or application of an l -serine synthesis inhibitor affected NK cell activation through Fos/Fosl. In summary, our findings reveal the role of bacteria-modulated serine metabolic signaling in NK cell activation and provide a novel therapeutic strategy to improve the efficacy of anti-PD1 immunotherapy in melanoma.

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“…For instance, p-N,N,N-trimethylammonioanilyl N′-hydroxysuccinimidyl carbamate iodide achieved subfemtomole to attomole levels of AAs detection [95]. Other examples (see Table 2 and Figure 4) [95][96][97][98][99][100][101][102][103][104][105] are dibenzyl ethoxymethylene malonate and benzyl ethyl ethoxymethylene malonate [105] as well as 4-dimethylaminobenzoylamido acetic acid N-hydroxysuccinimide ester [106] and dimethy-laminophenacyl bromide [107]. By synthesizing 3-aminopyridyl-N-hydroxysuccinimidyl carbamate (APDS) reagent, Shimbo et al [97] could measure over 105 amino compounds in rat plasma and quantify 22 AAs within 10 min.…”

Section: Hplc Tandem Msmentioning

confidence: 99%

Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

Yin

Adams

Schepdael

2023

J of Separation Science

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The significance of branched‐chain amino acids in diseases was clearly shown over the years. This review aims to describe the available techniques for their analytical determination. The article provides examples of the use of various analytical methods. The methods are divided into two categories: derivatization and non‐derivatization approaches. Separation is achieved through different chromatography or capillary electrophoresis techniques and can be combined with different detectors such as flame ionization, ultraviolet, fluorescence, and mass spectrometry. It compares the application of various derivatization reagents or detection as such for different detectors.

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Determination of amino acids in food and feed by microwave hydrolysis and UHPLC-MS/MS

Cited by 13 publications

References 31 publications

An Optimized Microwave-Assisted Digestion Method to Analyze the Amino Acids Profile of Quisqualis Fructus from Different Planted Origins

An Optimized Microwave-Assisted Digestion Method to Analyze the Amino Acids Profile of Quisqualis Fructus from Different Planted Origins

Eubacterium rectale Improves the Efficacy of Anti-PD1 Immunotherapy in Melanoma via l -Serine-Mediated NK Cell Activation

Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

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