Determination of aminoglycosides in pharmaceutical formulations — II. High-performance liquid chromatography

Fabre, H.; Sekkat, M.; Blanchin, Marie-Dominique; Mandrou, B.

doi:10.1016/0731-7085(89)80185-2

Cited by 48 publications

(19 citation statements)

References 27 publications

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“…Other authors have quantified gentamicin on the basis of the sum of 3 peaks. 25,26 The specificity of the method was established using forced degradation. The samples mixed with 0.1 mmol/L HCl showed no indication of degradation, and the gentamicin concentration remained essentially unchanged, with 99.63% ± 0.29% remaining for the HCl-treated samples and 99.10% ± 0.57% remaining for the control.…”

Section: Discussionmentioning

confidence: 99%

Stability of Trisodium Citrate and Gentamicin Solution for Catheter Locks after Storage in Plastic Syringes at Room Temperature

Cote

Battistella

Vercaigne

2010

CJHP

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Background: Catheter-related infections are a major problem for hemodialysis patients with central venous catheters for vascular access. Catheter lock solutions containing an anticoagulant are used to maintain the patency of the catheter between hemodialysis sessions. There is evidence that the use of lock solutions containing an antibiotic is associated with lower rates of infection but also that these solutions can kill microbes in colonized catheters and thus avoid the risks and costs associated with replacing the catheter.

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Section: Discussionmentioning

confidence: 99%

Stability of Trisodium Citrate and Gentamicin Solution for Catheter Locks after Storage in Plastic Syringes at Room Temperature

Cote

Battistella

Vercaigne

2010

CJHP

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“…Several methods have been described to determine netilmicin in pharmaceutical formulations and biological matrices. Precolumn derivatization LC with dansyl chloride [10], o-phthalaldehyde (OPA) [11][12][13][14], 1-fluoro-2,4-dinitrobenzene [15,16], 11-fluorenylmethyl chloroformate [17], or OPA-b-mercaptopropionic acid [18,19], and postcolumn LC derivatization with OPA [20,21] combined with fluorescence or UV detection have been described. However, these techniques are time consuming and give problems in quantification.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an improved reversed‐phase liquid chromatographic method combined with pulsed electrochemical detection for the analysis of netilmicin

Manyanga

Hoogmartens

Adams

2010

J of Separation Science

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Netilmicin is one of the aminoglycoside antibiotics that lacks a strong UV absorbing chromophore. However, the application of pulsed electrochemical detection has been used successfully for the direct analysis of aminoglycoside antibiotics. This study describes an improved LC method combined with pulsed electrochemical detection for the analysis of netilmicin. Using a Zorbax SB C-18 column (250 mm x 4.6 mm id, 5 microm), isocratic elution was carried out with a mobile phase containing sodium sulfate (20 g/L), sodium octanesulfonate (0.3 g/L), THF (20 mL/L), and 0.2 M phosphate buffer pH 3.0 (50.0 mL/L). The robustness of the method was examined by means of an experimental design. The method proved to be sensitive, repeatable, linear, and robust. The method has also been used to analyze some commercial netilmicin samples.

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“…Amikacin sulfate is quantitatively determined through various analytical techniques such as spectrophotometry, 4-8 spectroflourimetry 9-13 and HPLC methods. [14][15][16][17][18][19][20][21][22][23][24][25] The aminoglycoside antibiotic activity can be increased by association with Herbs like activity by Pityrogramma calomelanos (L.). 26 The spectroscopic method for assay of amikacin sulfate is not official in any pharmacopoeia.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of amikacin Sulfate using vanillin from pure and commercial brands available in Pakistan

Mughal¹,

Dayo²,

Ghoto³

et al. 2015

J YOUNG PHARM

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Objective: To determine the amikacin sulfate by developing a new spectrophotometric method by derivatization using double beam spectrophotometer from pure and commercial brands. Method: A quantitative analytical method was developed by amikacin sulfate using spectrophotometer after derivatization with vanillin under optimized parameters like pH, heating time and temperature, volume of reagent, Impact of mixing order, Effect of addition of solvents and Effect of excipients. The method was successfully applied on bulk and different brands containing amikacin sulfate. Results: The bulk and pharmaceutical analysis was carried out of different brands with formation of slight yellow colored imine base. The product showed absorbance at 400 nm w i t h molar absorptivity of 5.

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Determination of aminoglycosides in pharmaceutical formulations — II. High-performance liquid chromatography

Cited by 48 publications

References 27 publications

Stability of Trisodium Citrate and Gentamicin Solution for Catheter Locks after Storage in Plastic Syringes at Room Temperature

Stability of Trisodium Citrate and Gentamicin Solution for Catheter Locks after Storage in Plastic Syringes at Room Temperature

Development and validation of an improved reversed‐phase liquid chromatographic method combined with pulsed electrochemical detection for the analysis of netilmicin

Quantitative determination of amikacin Sulfate using vanillin from pure and commercial brands available in Pakistan

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