Determination of Ammonia and of Amide Nitrogen in Plant Tissue

Chemical Investigation of "Trashy" Leaf Phenomenon in Australian-Grown Flue-Cured Tobacco

“…Borsook and DubnotI 1939;Pucher, Vickery, and Leavenworth 1935;Lugg 1938;and others). In a careful study Shore, Wilson, and Stueck (1936) presented a reasonably clear picture of the formation" of NH3 during the period of acid ~ydrolysis, showing that the deamidation reaction, is accompanied by the slow ,deamination of peptides and amino acids.…”

“…A more detailed report of this investigation will be published elsewhere. The procedure adopted was as follows: Before use, the apparatus (see Pucher, Vickery, and Leavenworth 1935;Archibald 1943) is thoroughly aerated with air scrubbed with H 2S04 , The 100 ml. receiver flask is charged with 2 ml.…”

THE INFLUENCE OF LIGHT, TEMPERATURE, AND SOME ENZYME POISONS UPON THE TOTAL ORGANIC ACID CONTENT OF LEAF TISSUE OF KALANCHOE DAIGREMONTIANA (HAMET AND PERRIER)

“…In one experiment in the following studies various soluble nitrogen fractions were determined. Ammonia, total amide, and "glutamine" amide fractions were estimated using adaptations of the methods of PUCHER et al (33) and VICKERY et al (47). Total soluble nitrogen was estimated by an adaptation of the method of UMBREIT and BOND (43).…”

The Effect of Copper Deficiency on the Nitrogen Metabolism and Oil Synthesis of the Tung Tree

“…This fraction is most accurately called hydrolyzable reduced nitrogen. Since no ammonium nitrogen was detected in the original water extract by the vacuum distillation method of PUCHER et al (20), this fraction is probablv entirely amide nitrogen and will be so designated in this paper although specific amides were not isolated.…”

“…standard taper Erlenmeyer flask, 20 ml. of 12 N H2S04 added, and the solution was refluxed in a boiling water bath for 3 hours (20). The digest was then transferred to a Kjeldahl flask, washing(v five times with 60-ml.…”