Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation

Krasucka, D.; Kowalski, C.; Osypiuk, M.; Opielak, Grzegorz

doi:10.1556/achrom.27.2015.1.5

Cited by 11 publications

(5 citation statements)

References 17 publications

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“…Analysis of amoxicillin concentration in plasma was modified from Krasucka, Kowalski, Osypiuk, & Opielak, 2013. Samples were quantified by high-performance liquid chromatography (HPLC) with fluorescence detection.…”

Section: Drug Analysis Methods and Sample Processingmentioning

confidence: 99%

Pharmacokinetic profile of amoxicillin and its glucuronide‐like metabolite when administered subcutaneously to koalas (Phascolarctos cinereus)

Kimble

Vogelnest

Gharibi

et al. 2019

Vet Pharm & Therapeutics

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Amoxicillin was administered as a single subcutaneous injection at 12.5 mg/kg to four koalas and changes in amoxicillin plasma concentrations over 24 hr were quantified. Amoxicillin had a relatively low average ± SD maximum plasma concentration (Cmax) of 1.72 ± 0.47 µg/ml; at an average ± SD time to reach Cmax (Tmax) of 2.25 ± 1.26 hr, and an elimination half‐life of 4.38 ± 2.40 hr. The pharmacokinetic profile indicated relatively poor subcutaneous absorption. A metabolite was also identified, likely associated with glucuronic acid conjugation. Bacterial growth inhibition assays demonstrated that all plasma samples other than t = 0 hr, inhibited the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 to some extent. Calculated pharmacokinetic indices were used to predict whether this dose could attain a plasma concentration to inhibit some susceptible Gram‐negative and Gram‐positive pathogens. It was predicted that a twice daily dose of 12.5 mg/kg would be efficacious to inhibit susceptible bacteria with an amoxicillin minimum inhibitory concentration (MIC) ≤ 0.75 µg/ml such as susceptible Bordetella bronchiseptica, E. coli, Staphylococcus spp. and Streptococcus spp. pathogens.

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Section: Drug Analysis Methods and Sample Processingmentioning

confidence: 99%

Pharmacokinetic profile of amoxicillin and its glucuronide‐like metabolite when administered subcutaneously to koalas (Phascolarctos cinereus)

Kimble

Vogelnest

Gharibi

et al. 2019

Vet Pharm & Therapeutics

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“…A large number of available methods have been developed for determining penicillins from biological matrices, e.g . in milk, eggs, and plasma, but in the literature there is limited information about methods quantification of amoxicillin in medicated feedingstuffs with the use of the HPLC technique and extraction of AMO from the feed matrix ( 4 , 9 , 12 , 15 , 16 ). Researchers have often used acetonitrile, acetonitrile and water mixture or KH 2 PO 4 buffer for extraction amoxicillin from biological matrices at different pH ranges.…”

Section: Discussionmentioning

confidence: 99%

In-house validation method for quantification of amoxicillin in medicated feedingstuffs with the use of HPLC-DAD technique

Patyra

Kwiatek

2020

Journal of Veterinary Research

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AbstractIntroductionA high-performance liquid chromatographic–diode array detector (HPLC-DAD) method for the determination of amoxicillin in medicated feedingstuffs was developed and validated. The method was used to investigate the quality requirements of animal feedingstuffs (declared content of active substance and feed homogeneity).Material and MethodsTwo-gram samples were extracted by potassium phosphate buffer solution. Extracts were filtered and directly analysed by HPLC-DAD without further clean-up. Amoxicillin was separated by acetonitrile and 0.01M phosphate buffer (pH 5.0) on a Phenomenex Luna C18 column.ResultsThis method provided average recoveries of 76.1 to 81.6% with coefficients of variation (CV, %) for repeatability and reproducibility in the ranges of 3.7–7.2% and 5.3–7.6%, respectively. The limit of detection was 51.2 mg/kg and limit of quantification was 103.0 mg/kg.ConclusionThe method was successfully validated and proved to be efficient, precise, and useful for quantification of amoxicillin in medicated feedingstuffs.

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“…Thus, we choose BSTFA as the most suitable derivatization reagent. Derivatization medium should show excellent solubility to both derivatization reagents and the analytes since it is the external environment of derivatization reaction [16]. Therefore, selection of an appropriate medium is directly related to derivatization reaction.…”

Section: Derivatization Conditionsmentioning

confidence: 99%

Determination of cembrenediols in tobacco by gas chromatography—mass spectrometry-selected ion monitoring with precolumn derivatization

Zhou

Yang

et al. 2016

Acta Chromatographica

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Summary. An efficient and sensitive analytical method based on precolumn derivatization and gas chromatography-mass spectrometry-selected ion monitoring (GC-MS-SIM) was proposed and validated for analysis of two cembrenediols (CBDs) which are α-cembrenediol and β-cembrenediol in tobacco samples. CBDs in tobacco samples were extracted by sonication with 50 mL dichloromethane for 10 min before derivatized with 2:3 (v/v) bis(trimethylsilyl)trifluoroacetamide (BSTFA)-pyridine at 20 °C for 100 min. CBDs' level in tobacco samples was analyzed by GC-MS-SIM and quantified by the internal standard method. The linear range for α-CBD and β-CBD was 13.6-554.6 μg mL −1 and 4.11-162.6 μg mL −1 , and the correlation coefficients of both were 0.9998. The limit of detection (LOD) and limit of quantification (LOQ) of α-cembrenediol and β-cembrenediol were 0.40 μg g −1 and 1.34 μg g −1 , and 0.27 μg g −1 and 0.90 μg g −1 , respectively. Average recoveries of α-CBD and β-CBD were 94.4-99.9% and 91.9-98.2% while the relative standard deviations (RSDs, n = 5) were ranged from 2.67 to 5.6% and 2.04 to 4.22%, respectively. This proposed analytical method has been successfully applied to analyze CBDs in tobacco samples.

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Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation

Cited by 11 publications

References 17 publications

Pharmacokinetic profile of amoxicillin and its glucuronide‐like metabolite when administered subcutaneously to koalas (Phascolarctos cinereus)

Pharmacokinetic profile of amoxicillin and its glucuronide‐like metabolite when administered subcutaneously to koalas (Phascolarctos cinereus)

In-house validation method for quantification of amoxicillin in medicated feedingstuffs with the use of HPLC-DAD technique

Determination of cembrenediols in tobacco by gas chromatography—mass spectrometry-selected ion monitoring with precolumn derivatization

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