Determination of anabolic steroids in pharmaceuticals by liquid chromatography with a microemulsion of sodium dodecyl sulfate and pentanol as mobile phase

Cartas, Sagrario Torres; Garcı́a-Alvarez-Coque, M.C.; Camanas, R. M. Villanueava

doi:10.1016/0003-2670(94)00460-4

Cited by 51 publications

(3 citation statements)

References 17 publications

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“…In [69], progesterone, MPA, and some other steroids were chromatographed using microemulsions of 0.1 M sodium dodecylsulfate and 7% pentanol as a mobile phase.…”

Section: Liquid Chromatography and Hplcmentioning

confidence: 99%

Methods of control and standardization of drugs containing progestogen hormones (A review)

et al. 1998

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The group of progestogen hormones includes progesterone (corpus luteum hormone) and a number of synthetic preparations with a structure close to that of progesterone (norethisterone, lynestrenol, levonorgestrel, desogestrel, etc.).The process of state control of the commercial progestogen hormonal preparations and the corresponding normative documentation are not free of significant drawbacks (in particular, some documents operative in the Russian Federation do not regulate the contents of related steroids). For this reason, the problems pertaining to the increase in the level of standardization and control of the quality of progestogen hormonal preparations require permanent attention and thorough consideration.A lot of information concerning the analysis of progestogen hormones is contained in the monograph of G6rrg [1]. However, there are some new data accumulated since that publication.The main methods used for the analysis of progesterone and related drugs were reviewed in [2].An analysis of the published data shows that the authenticity of ethisterone [3][4][5], norethisterone [4,5], and hydroxyprogesterone capronate [5] is established using a color reaction with sulfuric acid; levonorgestrel is identified by its reaction with m-dinitrobenzene in alkaline medium [5]; ethis-I State Research Institute of Drug Standardization and Control, Ministry of Health and Medical Industry, Moscow, Russia.

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“…In [69], progesterone, MPA, and some other steroids were chromatographed using microemulsions of 0.1 M sodium dodecylsulfate and 7% pentanol as a mobile phase.…”

Section: Liquid Chromatography and Hplcmentioning

confidence: 99%

Methods of control and standardization of drugs containing progestogen hormones (A review)

et al. 1998

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“…Most attempts involved gas chromatography (GC) and high performance liquid chromatography (HPLC). However, the separation of anabolic steroids by GC requires further derivatization [4] and the separation efficiency is poor by HPLC [5,6]. Capillary electrophoresis has shown its superiority in recent year.…”

Section: Introductionmentioning

confidence: 99%

Separation of anabolic steroids by micellar electrokinetic capillary chromatography

1999

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SummaryThe separation of seven analogous anabolic steroids was studied by micellar electrokinetic capillary chromatography (MECC). The retention order was found to be dependent on polarity. All of these steroids were well separated by the addition of organic modifiers to the separation buffer. Of the organic modifiers tested, 1-propanol gave the best separation, better than methanol or acetonitrile.

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“…To date, MEEKC has been used for a variety of separations, e.g., natural products [9], sugars [10], fatty acids [11], hop acids [12], preservatives [13], bases and nucleosides [14], proteins [15], agrochemicals [16,17], ketones and diketones [6,18], steroids [19][20][21], vitamins [22][23][24][25][26], and a range of pharmaceuticals [23,[27][28][29][30][31][32][33][34][35]. All these separations involved standard solutions in relatively simple matrices.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of microemulsion electrokinetic capillary chromatography and micellar electrokinetic capillary chromatography for the analysis of UV‐absorbing compounds in human urine

Melin

Perrett

2004

Electrophoresis

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Separations of human urine by microemulsion electrokinetic chromatography (MEEKC) and micellar electrokinetic capillary chromatography (MEKC) with respect to resolution, migration times and efficiencies were optimized and compared. The optimised MEEKC and MEKC methods were simple and fast, both of which are excellent characteristics for the complex separations required in clinical and biomedical studies. However, resolution in MEKC was significantly greater than in MEEKC although migration times were 30% faster for the optimised MEEKC method. In addition, a faster analysis method (short-end injection) specifically for routine screening purposes was also investigated. With both MEEKC and MEKC modes, this provided short separations (less than 4 min for urine) with no major compromise in resolution. In conclusion, we found that MEEKC offered no real advantage over MEKC for urine analysis.

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Determination of anabolic steroids in pharmaceuticals by liquid chromatography with a microemulsion of sodium dodecyl sulfate and pentanol as mobile phase

Cited by 51 publications

References 17 publications

Methods of control and standardization of drugs containing progestogen hormones (A review)

Methods of control and standardization of drugs containing progestogen hormones (A review)

Separation of anabolic steroids by micellar electrokinetic capillary chromatography

A comparative study of microemulsion electrokinetic capillary chromatography and micellar electrokinetic capillary chromatography for the analysis of UV‐absorbing compounds in human urine

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