Determination of androstenone levels in porcine plasma by LC-MS/MS

Chen, G.; Ren, Li; Yang, Shi‐Yao; Qiu, Jun; Kamal‐Eldin, Afaf; Lundström, Kerstin

doi:10.1016/j.foodchem.2010.03.083

Cited by 9 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the within-laboratory reproducibility, the calculated RSD values (RSD < 10.5%) were below 20%, indicating good precision of the method (European Commission, 2002). Moreover, these validation parameters proved to perform equally well compared to other detection methods in serum or plasma such as HPLC-fluorescence detection (RSD 6 12.0%) Tuomola et al, 1996), LC-MS/MS (RSD 6 11.0%) (Chen et al, 2010) and a time-resolved fluoroimmunoassay (RSD 6 8.1%) (Tuomola et al, 1997).…”

Section: Accuracy and Precisionmentioning

confidence: 77%

“…These LOD and LOQ are noticeably lower compared to fat if a similar detection technique is considered (Bekaert et al, 2012), while for SK and IND, but not for AEON, slightly lower limits are reported by Fischer et al (2011) applying a stable isotope dilution analysis -headspace solid phase microextraction -gas chromatography/mass spectrometry. However, considering only serum or plasma specific detection techniques, the limits of this study are in accordance, although comparison is not straightforward due to aberrant criteria of determination of these limits Chen et al, 2010;Haugen, Brunius, & Zamaratskaia, 2012;Tuomola et al, 1997).…”

Section: Limit Of Detection and Limit Of Quantificationmentioning

confidence: 79%

See 1 more Smart Citation

Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry

et al. 2015

View full text Add to dashboard Cite

Section: Accuracy and Precisionmentioning

confidence: 77%

Section: Limit Of Detection and Limit Of Quantificationmentioning

confidence: 79%

Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry

et al. 2015

View full text Add to dashboard Cite

“…Although it is not known whether AND undergoes enterohepatic circulation, showed that hepatocytes produce sulphoconjugates and glucuronyl conjugates of AND; also found that increased levels of sulphoconjugation decreased the accumulation of AND in adipose tissue. Chen et al (2010) also reported that the majority of plasma AND was in the form of conjugates. If AND follows a similar route of metabolism and recycling as oestrogens, it may be possible to decrease AND levels by interrupting the enterohepatic circulation.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of non-nutritive sorbent materials as intestinal-binding agents for the control of boar taint

Jen

Squires

2011

Animal

View full text Add to dashboard Cite

In many countries, male pigs are castrated to prevent boar taint, but this practice raises concerns about animal welfare and reduces the production efficiency of pork. The objective of this study was to develop dietary manipulations to prevent boar taint. We evaluated the effectiveness of adding activated carbon (AC) or Tween-60 (Tween; polyoxyethylene sorbitan monostearate) to pig finishing diets to reduce levels of androstenone (AND) and skatole in plasma and fat of entire male pigs. Boars (159 6 2 days of age at the start of the experiment) were fed diets supplemented with either 5% AC or 5% Tween for 28 days followed by either 14 or 28 days of recovery. Plasma samples were collected at experimental days 0, 7, 14, 21, 28, 42 and 56, and backfat biopsies were taken at experimental days 0, 28, 42 and 56. Feeding AC significantly (P , 0.05) reduced the levels of AND in plasma by day 28 compared to day 0 and by day 42 in fat compared to day 0. AC treatment also decreased levels of oestrone sulphate (E 1 S) in plasma by day 7 compared to day 0. Treatment with Tween significantly decreased (P , 0.05) the levels of plasma AND by day 28 from levels at day 0. Tween treatment did not significantly affect levels of fat AND or plasma E 1 S compared to day 0; however, fat AND levels decreased between days 28 and 42 following treatment with Tween (P , 0.05). Levels of plasma E 1 S, plasma AND and fat AND for control boars remained constant throughout the experiment. Skatole plasma concentrations were very low and did not vary significantly (P . 0.05) from day 0 for any treatment, but fat skatole levels decreased by day 42 in the Tween treatment group. Importantly, there was no difference in growth rate between the control and experimental groups. We conclude that adding AC or Tween to finishing diets for boars can reduce the levels of plasma and fat AND, but further work is needed to confirm the effects of these treatments on reducing fat skatole levels.

show abstract

“…Besides the animals' benefit from the abandonment of castration, raising entire male pigs is more profitable from economic aspects. It is reported that entire boars show higher nutrient uptake efficiency and superior meat quality because of leaner carcasses and higher protein content when compared with castrates . In consequence, an increased amount of meat from entire male pigs should end up in the food chain.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of deuterium‐labeled d₃‐androstenone and d₃‐skatole for boar taint analysis

Fischer

Elsinghorst

Wüst

2011

Labelled Comp Radiopharmac

View full text Add to dashboard Cite

The steroidal pig pheromone androstenone (5α-androst-16-en-3-one) as well as the indole derivate skatole are known to be the major compounds contributing to boar taint. The preparation of 2 H-labeled internal standards of androstenone and skatole for stable isotope dilution assay-gas chromatography-mass spectrometry analysis of pig back fat samples is presented. The synthesis of d 3 -androstenone is highlighted by a palladium catalyzed deuteration of a Δ5,6-double bond of a hydrophobic steroid in a polar, D 2 O-containing solvent mixture. Moreover, the utilization of D 2 O as a deuterium source with in situ formation of D 2 by means of Mg 0 was found to be a very feasible and convenient alternative with respect to costs and technical effort.

show abstract

Determination of androstenone levels in porcine plasma by LC-MS/MS

Cited by 9 publications

References 23 publications

Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry

Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry

Efficacy of non-nutritive sorbent materials as intestinal-binding agents for the control of boar taint

Synthesis of deuterium‐labeled d₃‐androstenone and d₃‐skatole for boar taint analysis

Contact Info

Product

Resources

About

Determination of androstenone levels in porcine plasma by LC-MS/MS

Cited by 9 publications

References 23 publications

Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry

Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry

Efficacy of non-nutritive sorbent materials as intestinal-binding agents for the control of boar taint

Synthesis of deuterium‐labeled d3‐androstenone and d3‐skatole for boar taint analysis

Contact Info

Product

Resources

About

Synthesis of deuterium‐labeled d₃‐androstenone and d₃‐skatole for boar taint analysis