Determination of anionic surfactants during wastewater recycling process by ion pair chromatography with suppressed conductivity detection

Levine, Lanfang H.; Judkins, Jennifer; Garland, Jay L.

doi:10.1016/s0021-9673(00)00155-2

Cited by 33 publications

(8 citation statements)

References 5 publications

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“…Plate contents were incubated at 30°C, and readings were obtained every 15 min for 120 h. Plates were not shaken during the incubation period. On day 21, a single pulse of 400 ml of a 1,000-ppm SLES and 1,000-ppm CAPB solution was added to all treatments to determine surfactant decay rates based on analysis of nutrient solution for SLES by using ion pair chromatography with suppressed conductivity detection (11) or for CAPB by using high-pressure liquid chromatography linked to electrospray ionization-mass spectrometry (10).…”

mentioning

confidence: 99%

Community-Level Physiological Profiling Performed with an Oxygen-Sensitive Fluorophore in a Microtiter Plate

Garland

Roberts

Levine

et al. 2003

Appl Environ Microbiol

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Community-level physiological profiling based upon fluorometric detection of oxygen consumption was performed on hydroponic rhizosphere and salt marsh litter samples by using substrate levels as low as 50 ppm with incubation times between 5 and 24 h. The rate and extent of response were increased in samples acclimated to specific substrates and were reduced by limiting nitrogen availability in the wells.Characterization and classification of heterotrophic microbial communities based on rapid assessment of multiple solecarbon-source use, termed community-level physiological profiling (CLPP) by Lehman et al. (9), were developed over 10 years ago (6). Although CLPP is a valuable method for assessing relative change in microbial communities when properly applied (3, 12), limitations due to selective enrichment resulting from the high substrate concentrations (i.e., Ͼ100 mM) and long incubation periods (typically 1 to 4 days) restrict its present use for assessing actual differences in the physiological capabilities of microbial communities (2,8,14,16). Development of more functionally relevant CLPP will depend on the application of new detection methods that allow for rapid and direct (e.g., O 2 consumption or CO 2 production) rather than indirect (e.g., tetrazolium dye reduction) assessment of respiration, with decreased requisite incubation times and substrate concentrations. In addition, the use of undefined, proprietary reagents should be minimized so that incubation conditions can be defined and manipulated by the investigator.14 C respirometric approaches limit enrichment effects by allowing for detection of physiological response with addition of very low substrate concentrations (i.e., nanomolar levels), but even simplified assay systems (13) still have the expense associated with disposal of radioactive wastes. CLPP approaches based on CO 2 monitoring that limit incubation time have been developed (1) but require high substrate concentrations (i.e., 10 to 100 mM).Tracking the rate of O 2 consumption in the test medium may improve sensitivity, given the relatively low concentration and solubility of O 2 in water. A recently developed, fluorescencebased microplate platform for assessing dissolved oxygen (BD Oxygen Biosensor System; BD Biosciences, Bedford, Mass.) (17) could enable rapid testing of multiple substrates; the effectiveness of this system to detect known shifts in substrate utilization by mixed microbial communities is the subject of this work. The BD Oxygen Biosensor System is based on an O 2 -sensitive fluorophore, 4,7-diphenyl-1,10-phenathroline ruthenium (II) chloride, absorbed into a silicone matrix that is permeable to O 2 (17). The fluorescence of the ruthenium dye is quenched by the presence of O 2 , so the signal from the fluorophore-gel complex loaded on the bottom of the microplate wells increases in response to respiration in the overlying sample. In this work, samples of environmental systems suspended in sterile phosphate-buffered mineral salts (PBMS) ( Ϫ1 ) were inoculated into the ...

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mentioning

confidence: 99%

Community-Level Physiological Profiling Performed with an Oxygen-Sensitive Fluorophore in a Microtiter Plate

Garland

Roberts

Levine

et al. 2003

Appl Environ Microbiol

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“…Several methods of determining anionic surfactants exist: titration [1,2], voltamperometry [3], spectrophotometry [1,[3][4][5][6], sensor-based methods [7], flow injection analysis [8][9][10] or chromatography [11][12][13][14]. The use of high performance liquid chromatography allows for both qualitative and quantitative analysis of individual surfactants present in mixtures.…”

Section: Introductionmentioning

confidence: 99%

“…The use of high performance liquid chromatography allows for both qualitative and quantitative analysis of individual surfactants present in mixtures. Depending on the surfactant, such analysis may be carried out with the use of different separation methods, such as reverse-phase or ion-exchange chromatography [11][12][13][14]. Chromatography-based methods of determining anionic surfactants are more sensitive and precise compared to standard methods.…”

Section: Introductionmentioning

confidence: 99%

Continuous Flow Methylene Blue Active Substances Method for the Determination of Anionic Surfactants in River Water and Biodegradation Test Samples

Wyrwas

Zgoła‐Grześkowiak

2013

J Surfact & Detergents

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Anionic surfactants are commonly determined with the use of the methylene blue active substances (MBAS) standard method, which is time-consuming and labor-intensive. Therefore, new methods for determination of anionic surfactants are needed. In this study, the standard MBAS method for determination of anionic surfactants was modified and adjusted to work in a continuous flow system combined with spectrophotometric measurement. The developed method was found to be satisfactory in terms of sensitivity and precision, with a short time of analysis. The quantification limit for anionic surfactants was at 16 μg L−1, with a relative standard deviation of 1.3 % for a model sample and 3.8 % for a river water sample. The results obtained for environmental samples were comparable to those obtained by using the standard MBAS method; however, the developed continuous flow method is faster, more sensitive and consumes smaller doses of chemical reagents.

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“…Anionic surfactants are the most widely used surfactants in personal care products. Surfactants used in personal care preparations account for an estimated 15-16% of the total surfactant consumption [2,3]. In 2010, the total amount of surfactants (not including soaps) consumed in Europe was 2.94 Mt, of them 1.397 Mt were non-ionic, 1.193 Mt were anionic, 0.250 Mt were cationic and 0.096 Mt were amphoteric, according to statistics published by the European Committee of Surfactants and their Organic Intermediates (CESIO) [4].…”

Section: Introductionmentioning

confidence: 99%

Determination of alcohol sulfates and alcohol ethoxysulfates in wastewater samples by liquid chromatography tandem mass spectrometry

Fernández-Ramos

Ballesteros

Blanc

et al. 2013

Microchemical Journal

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Determination of anionic surfactants during wastewater recycling process by ion pair chromatography with suppressed conductivity detection

Cited by 33 publications

References 5 publications

Community-Level Physiological Profiling Performed with an Oxygen-Sensitive Fluorophore in a Microtiter Plate

Community-Level Physiological Profiling Performed with an Oxygen-Sensitive Fluorophore in a Microtiter Plate

Continuous Flow Methylene Blue Active Substances Method for the Determination of Anionic Surfactants in River Water and Biodegradation Test Samples

Determination of alcohol sulfates and alcohol ethoxysulfates in wastewater samples by liquid chromatography tandem mass spectrometry

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