Determination of anthelmintic drug residues in milk using ultra high performance liquid chromatography–tandem mass spectrometry with rapid polarity switching

Whelan, Michelle; Kinsella, Brian; Furey, Ambrose; Moloney, Mary; Cantwell, Helen; Lehotay, Steven J.; Danaher, Martin

doi:10.1016/j.chroma.2010.05.007

Cited by 153 publications

(111 citation statements)

References 32 publications

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“…There are very few reported data on CC α and CC β values. The CC α and CC β values determined using UHPLC-MS/MS analysis were lower than those reported here, and ranged from 0.14 to 1.9 and 11 to 123 µg kg -1 for unapproved and MRL substances, respectively (Whelan et al, 2010).…”

Section: Resultsmentioning

confidence: 38%

Determination of benzimidazole residues and their metabolites in raw milk using high performance liquid chromatography-diode array detection

et al. 2017

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A new analytical method using high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the analysis of 18 benzimidazoles and their metabolites in milk. Samples were extracted with acetonitrile and n-hexane and purified by polymer cation exchange (PCX) solid phase extraction cartridges. LC separation was performed on Xbridge C18 with gradient elution using acetonitrile and ammonium acetate buffer. The DAD detection was set at 298, 312, 254 and 290 nm. The method was validated according to the criteria of Commission Decision 2002/657/EC. The following validation parameters were set: accuracy (expressed as recovery) 31.7-137.6 %, limit of decision (CCα) 6.0-120.6 µg kg -1 , detection capability (CCβ) 6.1-120.8 µg kg , precision as CV 7.0-22.5 %, withinlaboratory reproducibility expressed as CV 8.8-30.6 %. Finally, the developed method was applied to the analysis of collected milk samples. A total of 50 milk samples was analysed for benzimidazole residues. All obtained concentrations for all compounds were below the LOQ values.

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Section: Resultsmentioning

confidence: 38%

Determination of benzimidazole residues and their metabolites in raw milk using high performance liquid chromatography-diode array detection

et al. 2017

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“…The method used for the detection of NIT has been developed previously (Whelan et al, 2010) for 38 anthelmintic residues in milk. In this study the samples were only monitored for NIT.…”

Section: Resultsmentioning

confidence: 99%

Research of nitroxynil residues in bovine milk following a single administration in the dry period by ultra-performance liquid chromatography tandem mass spectrometry

et al. 2013

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). Thirty-five pregnant dairy cows were treated in this study with nitroxynil (340 mg/mL solution for injection) at the recommended dose of 10 mg/kg body weight at the start of the dry period, i.e. 53 to 74 days before the expected calving. Calving occurred between 43 days and 79 days after treatment. The concentrations of NIT in the milk were monitored for up to 120 days after calving. NIT residues were extracted using acetonitrile; magnesium sulfate and sodium chloride were added to induce liquid-liquid partitioning and purified by dispersive solid phase extraction for clean-up. NIT was detected by ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) in negative ionization mode. The highest concentrations of this drug were found in two animals at the first milking, 48 and 53 day post treatment with levels of 362 and 657 µg kg -1 , respectively. NIT residues were below the limit of detection of the method (0.24 µg/kg -1 ) between 67 and 106 day post-treatment. Following calving, residues rapidly depleted in animals and were non-detectable from 10 to 38 days post-calving. In particular, in all animals milk resulted compliant (<20 µg/kg −1 ) three days post partum.

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“…The SPE is one of the analysis methods that are widely used recently to increase the selectivity of target substances compared to LLE [22,23]. For instance, Gentili et al [24] have reported that the recovery rate of TA in Cattle was high (97~99%) by LC-MS in combination with SPE [24].…”

Section: Optimization Of Sample Preparation Stepmentioning

confidence: 99%

Development of an Improved HPLC-PDA Method for the Determination of Tolfenamic Acid in Meat and Milk

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et al. 2017

J Chromatogr Sep Tech

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Determination of anthelmintic drug residues in milk using ultra high performance liquid chromatography–tandem mass spectrometry with rapid polarity switching

Cited by 153 publications

References 32 publications

Determination of benzimidazole residues and their metabolites in raw milk using high performance liquid chromatography-diode array detection

Determination of benzimidazole residues and their metabolites in raw milk using high performance liquid chromatography-diode array detection

Research of nitroxynil residues in bovine milk following a single administration in the dry period by ultra-performance liquid chromatography tandem mass spectrometry

Development of an Improved HPLC-PDA Method for the Determination of Tolfenamic Acid in Meat and Milk

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