Determination of Anthocyanins of Purple Carrot Two Cultivars

Abstract

Two cultivars of purple carrot 'Pupur' and 'Mayami shokoladnaya" were investigated. Anthocyanins of both cultivars was qualitatively the samemain compound was cyanidin-3-feruloylhexosyl-pentosyl-hexodide, mole fraction of the anthocyanin was approximately 72 % of the overall anthocyanin content of carrot roots of cv. "Purpur" (I) and only 49 % in in carrot roots of cv. "Mayami shokoladnaya" (II). Cyanidin-3-pentosyl-hexoside (33.8 %) was found in carrot roots of (II) and only 4-18 % in carrot roots of (I). The overall accumulation of anthocyanins was fond in roots of carrots was 0.111 and 0.232 g per 100 g FW for cultivars I and II, respectively.

Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases

“…Several papers report the determination of anthocyanins in diverse biological samples such as plants extracts, food, and agricultural samples [ 9 , 31 , 32 , 34 , 49 , 59 , 60 , 61 ]. The spectrophotometric quantification of total monomeric anthocyanins is based on the characteristic light absorbance that these compounds possess depending on their ionization state [ 1 ].…”

“…The most used and useful HPLC and UHPLC methods for flavonoid analyses utilize reverse phase columns, in general C18 silica bounded stationary phases, of different diameters and particle size. Common 5 µm particle size HPLC columns are still wide spread in most of the analytical laboratories and still possess good selectivity and resolution of complex mixture of anthocyanidins and anthocyanins [ 40 , 57 , 59 , 60 , 68 , 69 ]. The upgrade to more powerful columns with smaller diameters and particle size (2.6 or 1.7 µm) is limited in many cases to the availability of UHPLC instruments and more modern chromatographic equipment.…”