Determination of anthracene by real-time immuno-polymerase chain reaction assay

Zhuang, Huisheng; Zhou, Chun

doi:10.1016/j.aca.2008.10.077

Cited by 26 publications

(6 citation statements)

References 33 publications

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“…persistent, bioaccumulative and toxic to human health and the environment. 9 Numerous traditional methods have been developed to identify PAHs such as gas chromatography (GC) coupled with mass spectrometry (GC/MS), 10 High Performance Liquid Chromatography (HPLC) 11 and spectroscopy. 12 Despite their high sensitivity and specificity, these compounds require skills to handle.…”

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confidence: 99%

Surface Modification of Screen-Printed Carbon Electrode (SPCE) with Calixarene-Functionalized Electrochemically Reduced Graphene Oxide (ERGO/C4) in the Electrochemical Detection of Anthracene

Zainal

Ahmad

Ngee

2019

J. Electrochem. Soc.

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In this study, electrochemically reduced graphene oxide (ERGO) and 4-tertbutylcalix[4]arene (C4) were deposited on the surface of screen-printed carbon electrodes (SPCE) to detect anthracene in aqueous solution. The formation of electrochemically reduced graphene oxide/calix[4]arene (ERGO/C4) on SPCE was characterized using Raman spectroscopy, FTIR, and FESEM. Electrochemical properties of the modified electrode were investigated by means of CV and DPV. The electrochemical response of anthracene was greatly improved on ERGO/C4-SPCE compared to bare SPCE. Under optimized condition, the anthracene peak showed a good linear relationship at a concentration ranging from 2-8 nM and a detection limit of 0.016 nM. The developed sensor was able to conserve its performance in the presence of various organic compound and inorganic metal ions and also yielded a good comparable result to the conventional method which is HPLC in real sample with acceptable recovery of 96-97%; thus proving to be a promising candidate for rapid, inexpensive, and sensitive determination of anthracene in real samples.

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confidence: 99%

Surface Modification of Screen-Printed Carbon Electrode (SPCE) with Calixarene-Functionalized Electrochemically Reduced Graphene Oxide (ERGO/C4) in the Electrochemical Detection of Anthracene

Zainal

Ahmad

Ngee

2019

J. Electrochem. Soc.

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“…We have adopted this method for the quantication of antibodies to the environmental pollutants of low molecular weight such as uoranthene, anthracene, as well as the PCB congeners PCB77 and PCB37. [21][22][23][24] Good detection results are obtained.…”

Section: Introductionmentioning

confidence: 81%

Determination of multi-residue PCBs in air by real-time fluorescent quantitative immuno-PCR assay

2014

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Amplification curves of a dilution series of Aroclor 1248 of direct competition rt-IPCR. In the figure, the fluorescence signal of the curve (10 fg mL−1) reaching the threshold was at around cycle 12.3, and there was a fall in the Ct value from 10 to 106 fg mL−1. This result implied that the time expended to reach the threshold for the high concentration of PCB molecules was much longer than for the low concentration PCBs.

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“…The possibility of labelling the secondary antibody with a nucleotide sequence and to implement sequence amplification using PCR has been explored (Sano et al 1992). This approach improves the sensitivity of ELISA tests by a factor of 100-10,000 and has been widely used for the detection of rare infectious diseases (Guo et al 2006), toxins in food (Kuczius et al 2009;Zhang et al 2010) or pollutants in aqueous media (Zhuang and Zhou 2009;Chen and Zhuang 2009). However, PCR requires the implementation of complex protocols that need special instruments and qualified personnel.…”

Section: Isothermal Dna Amplification Methodsmentioning

confidence: 99%