Determination of Anti-Bartonella henselae Antibody by Indirect Fluorescence Antibody Test

Tsuneoka, Hidehiro; Fujii, R; Yamamoto, Kiyomi; Fujisawa, Kazuo; Iino, Hidechika; Matsuda, Masako; Tsukahara, Masato

doi:10.11150/kansenshogakuzasshi1970.72.801

Cited by 15 publications

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“…The presence of IgM in patients is clear evidence of recent contact with B. henselae; however, the sensitivity of assays in the detection of the IgM is low (5)(6)(7)(8)(9)(10)12). IgM remains in the serum for approximately 3 months (7,13).…”

Section: Discussionmentioning

confidence: 99%

“…The antibody titer was expressed as the highest dilution of serum that yielded a positive staining result. Sera with IgG titers of Ն1:256 or IgM titers of Ն1:20 were regarded as positive, whereas sera with IgG titers of Ͻ1:256 or IgM titers of Ͻ1:20 were regarded as negative (5,15). The IFAs were performed by one technician.…”

Section: Methodsmentioning

confidence: 99%

“…The slides for the IgM-IFA were prepared using B. henselae ATCC 49882 without cocultivation with Vero cells. The details of the procedures have been described elsewhere (5,14). The antibody titer was expressed as the highest dilution of serum that yielded a positive staining result.…”

Section: Methodsmentioning

confidence: 99%

“…henselae IgG is regarded as the gold standard for detecting antibodies to B. henselae. However, although this method has high specificity, the sensitivity varies widely in different reports (4)(5)(6). Although the detection of IgM antibodies is crucial for the diagnosis of active B. henselae infection, the sensitivity of IFA for anti-B.…”

mentioning

confidence: 99%

“…Although the detection of IgM antibodies is crucial for the diagnosis of active B. henselae infection, the sensitivity of IFA for anti-B. henselae IgM (IgM-IFA) is reported to be especially low (5)(6)(7)(8).…”

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confidence: 99%

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Development of a Highly Specific IgM Enzyme-Linked Immunosorbent Assay for Bartonella henselae Using Refined N -Lauroyl-Sarcosine-Insoluble Proteins for Serodiagnosis of Cat Scratch Disease

et al. 2016

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The conventional anti-Bartonella henselae IgM enzyme-linked immunosorbent assay (IgM-ELISA) methods for diagnosing cat scratch disease (CSD) remain poor in both sensitivity and specificity. We sought to develop an IgM-ELISA with improved accuracy in the serodiagnosis of CSD by exploring the antigens that are most suitable for an ELISA. We prepared 5 different protein antigens: antigen I (sonicated B. henselae whole-cell antigen), antigen II (N-lauroyl-sarcosine-insoluble antigen), antigen III (processed sarcosine-soluble antigen), and antigen IV and antigen V (sarcosine-insoluble and sarcosine-soluble antigens refined by DEAE-Sepharose Fast Flow ion-exchange chromatography). The IgM antibodies in the sera of 47 patients with clinically suspected CSD (24 definite, 23 suspected) and of 85 healthy individuals were examined by ELISAs using the 5 antigens, and the results were compared with those of an IgM indirect fluorescent antibody assay (IgM-IFA). In a reference panel, which consisted of 5 positive and 5 negative sera, antigen I and antigen III failed to distinguish between the two statuses, whereas the other three antigens succeeded in distinguishing between them. When the cutoff value was set at the 98th percentile of the ELISA index for healthy individuals, the sensitivity of IgM-IFA for the 24 cases of definite CSD was 54%, whereas the sensitivities of the IgM-ELISAs with antigen II, IV, and V were 75%, 83%, and 75%, respectively. The sensitivities of these three IgM-ELISAs for all 47 of the clinically suspected cases were 49%, 64%, and 51%, respectively. In contrast, the sensitivity of IgM-IFA was 28%. These results indicate that the refined sarcosine-insoluble proteins (antigen IV), which possessed the highest specificity among the 5 antigens, are the most appropriate for developing an IgM-ELISA for the highly specific serodiagnosis of CSD.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Development of a Highly Specific IgM Enzyme-Linked Immunosorbent Assay for Bartonella henselae Using Refined N -Lauroyl-Sarcosine-Insoluble Proteins for Serodiagnosis of Cat Scratch Disease

et al. 2016

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Serodiagnosis of Cat Scratch Disease: Response to Bartonella henselae in Children and a Review of Diagnostic Methods

Sander¹,

Berner

Ruess

2001

EJCMID

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In this study, sera from 116 children without clinical evidence of cat scratch disease (CSD) and sera from 19 children with CSD were investigated by two different immunofluorescent assays (test A and test B) to evaluate the seroprevalence of Bartonella henselae antibodies in this population. Antibodies against Bartonella henselae were found in noninfected children only in low titers: 13% and 3.5% of serum samples were positive by test A and test B, respectively. IgG titers as determined by test A ranged from 1:64 to 1:256, whereas test B did not yield titers >1:64. Of the 19 children with CSD, 7 (37%) and 5 (26%) had low antibody titers (1:64-1:256) on admission by test A and test B, respectively. In such cases, IgM antibodies against Bartonella henselae and/or a titer increase in the following weeks are required to prove suspected CSD. The results indicate that the seroprevalence of Bartonella henselae antibodies in children without evidence of CSD is low, and that the results may differ depending on the tests being used. Low antibody titers, however, were found not only in healthy children but also among patients with CSD, which could be indicative of the beginning or of the end of an illness. Currently, only few data are available concerning the seroprevalence of antibodies to Bartonella spp. among children. Although children are often affected by CSD, the specificity of most tests has been evaluated by investigating blood donors, who are usually adults. Several different serological tests are used for the diagnosis of CSD, including the immunofluorescence assay, the enzyme-linked immunosorbent assay, and Western blot (WB) analysis. The sensitivities of different IFAs range from 14 to 100%, depending on the antigen used, the cut-off chosen, and the test procedures. The current diagnostic value of different serological tests for diagnosis of CSD is reviewed.

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Bartonella spp. bacteremia in high-risk immunocompetent patients

Maggi

Mascarelli

Pultorak

et al. 2011

Diagnostic Microbiology and Infectious Disease

146

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Determination of Anti-Bartonella henselae Antibody by Indirect Fluorescence Antibody Test

Cited by 15 publications

References 0 publications

Development of a Highly Specific IgM Enzyme-Linked Immunosorbent Assay for Bartonella henselae Using Refined N -Lauroyl-Sarcosine-Insoluble Proteins for Serodiagnosis of Cat Scratch Disease

Development of a Highly Specific IgM Enzyme-Linked Immunosorbent Assay for Bartonella henselae Using Refined N -Lauroyl-Sarcosine-Insoluble Proteins for Serodiagnosis of Cat Scratch Disease

Serodiagnosis of Cat Scratch Disease: Response to Bartonella henselae in Children and a Review of Diagnostic Methods

Bartonella spp. bacteremia in high-risk immunocompetent patients

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