Determination of anti-staphylococcal activity of thymoquinone in combinations with antibiotics by checkerboard method using EVA capmat™ as a vapor barrier

Rondevaldova, Johana; Nový, Pavel; Urban, Jan; Kokoška, Ladislav

doi:10.1016/j.arabjc.2015.04.021

Cited by 20 publications

(12 citation statements)

References 23 publications

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“…Volatility causes a risk of active substance losses via evaporation during sample handling, experiment preparation, and incubation, depending on time and temperature conditions [40,41]. Although, in conventional assays based on disc diffusion and dilution tests, this can be prevented by using vapour barriers such as an ethylene vinyl acetate (EVA) capmat [42] and a cover glass with a plastic seal ring [43], this approach does not solve the problem of the assessment of the antimicrobial potential of VAs in a vapour phase. In this case, the interaction of VAs with the matrix onto which they are applied (e.g., paper disc, cultivation broth) is a crucial aspect affecting the speed and intensity of their evaporation into the atmosphere.…”

Section: Asteraceae Shoot Disc Volatilization Methodsmentioning

confidence: 99%

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Houdková

Kokoška

2020

Planta Med

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This review summarizes data on the in vitro antimicrobial effectiveness of volatile agents of plant origin and in vitro methods for evaluating their activity in the vapour phase. As a result of literature analysis, the antimicrobial efficacy of vapours from 122 different plant species and 19 pure compounds examined in 61 studies using different in vitro tests against a broad spectrum of microorganisms was identified and summarized. In addition, 11 different techniques found in the literature are described in detail. An original classification of methods based on the solid and liquid matrix volatilization principle is proposed because carrier medium/matrix selection is crucial for the volatilization of any agents tested. This review should be useful for medicinal, pharmaceutical, food, and agricultural experts working in areas related to the management of infectious diseases (especially respiratory and skin infections), food preservation (active packaging), and protection of agriculture products (controlled atmosphere). It may also stimulate the interest of pharmaceutical, cosmetic, food, and agriculture industries in the research and development of new antimicrobial agents of natural origin. Since several original apparatuses previously developed for antimicrobial susceptibility testing in the vapour phase are described in this review, labware manufacturers may also be interested in this topic. The review also provides specific guidelines and recommendations for researchers studying the antimicrobial activity of volatile agents. The article will therefore appeal to communities of industrial stakeholders, pharmacists, physicians, food experts, agriculturists, and researchers in related areas such as pharmacology, medicinal chemistry, microbiology, natural product chemistry, food preservation and plant protection.

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Section: Asteraceae Shoot Disc Volatilization Methodsmentioning

confidence: 99%

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Houdková

Kokoška

2020

Planta Med

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“…Three clinical isolates of drug resistant S. aureus (SA1, SA2, and SA3) and one epidemic MRSA strain (EMRSA-15) were obtained from the Motol University Hospital (Prague, CZ). Oxacillin, gentamicin, tetracycline, and penicillin were used as markers of the antibiotic resistance as it has been previously determined 97 – 99 . Based on the MIC values, clinical isolates were identified to be resistant to: SA1—resistant to gentamicin (MIC 8 µg/mL) and tetracycline (MIC 8 µg/mL); SA2—resistant to oxacillin (MIC 68 µg/mL), gentamicin (MIC 16 µg/mL) and tetracycline (MIC 8 µg/mL); SA3—resistant to gentamicin (MIC 8 µg/mL) and penicillin (MIC 18.67 µg/mL); EMRSA-15—resistant to oxacillin (MIC 99.56 µg/mL) and penicillin (MIC 16 µg/mL).…”

Section: Methodsmentioning

confidence: 99%

In vitro antagonistic inhibitory effects of palm seed crude oils and their main constituent, lauric acid, with oxacillin in Staphylococcus aureus

Laloučková

Skřivanová

Rondevaldova

et al. 2021

Sci Rep

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Infections caused by Staphylococcus aureus are a serious global threat, and with the emergence of antibiotic resistance, even more difficult to treat. One of the possible complications in antistaphylococcal therapy represents negative interactions of antibiotics with food. In this study, the in vitro interaction between oxacillin and crude palm seed oil from Astrocaryum vulgare, Cocos nucifera, and Elaeis guineensis against nine strains of S. aureus was determined using the checkerboard method. Lauric acid was identified as a major constituent of all tested oils by gas chromatography. The results showed strong concentration dependent antagonistic interactions between palm oils and oxacillin with values of fractional inhibitory concentrations indices ranging from 4.02 to 8.56 at concentrations equal or higher than 1024 µg/mL of the tested oils. Similarly, lauric acid in combination with oxacillin produced antagonistic action with fractional inhibitory concentration indices ranging from 4.01 to 4.28 at 1024 µg/mL. These findings suggest that interference between oxacillin and palm oils and their constituents can negatively affect the treatment of staphylococcal infections in humans and other animals.

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“…The antibacterial effect synergism of N. sativa seed essential oil with antibiotic was reported in several previous studies, therefore it may be used in combination with antibiotic. 28,46 Further investigation is needed in order to investigate the cytotoxic effect of N. sativa seed o/w emulsion regarding to human use.…”

Section: Discussionmentioning

confidence: 99%

Untitled

2017

IJPSR

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Our previous study highlighted the antimicrobial activity evaluation of Nigella sativa seed oil extract that was obtained from supercritical fluid extraction using carbon dioxide (SCFE-CO 2 ) on Gram positive pathogenic bacteria. The aim of this study was to formulate oil-in-water (o/w) emulsion of previous N. sativa seed oil extract and to investigate its antimicrobial activity against Streptococcus mutans and Streptococcus sanguis as the most common causative bacteria of dental caries. Oil-in-water emulsion containing 10% N. sativa seed oil extract (DLBS1355) exhibited good inhibition activity on both S. mutans and S. sanguis. Time-kill assay and biofilm inhibition assay were conducted using emulsion with 0.5% N. sativa seed oil extract content. The results showed bactericidal and biofilm inhibition activities on both tested bacteria. Antimicrobial activity of the emulsion was found higher on S. sanguis than S. mutans. Results of the present study showed that emulsification process did not significantly affect the antimicrobial activity of N. sativa seed oil extract and the formulated N. sativa oil-in-water emulsion could further be used as an antimicrobial agent against dental cariogenic bacteria.

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Determination of anti-staphylococcal activity of thymoquinone in combinations with antibiotics by checkerboard method using EVA capmat™ as a vapor barrier

Cited by 20 publications

References 23 publications

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

In vitro antagonistic inhibitory effects of palm seed crude oils and their main constituent, lauric acid, with oxacillin in Staphylococcus aureus

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