Determination of antibacterial activity and metabolite profile of Ruta graveolens against Streptococcus mutans and Streptococcus sobrinus

Salman, Hamzah Abdulrahman; Venkatesh, Sankarasetty; Senthilkumar, R; Kumar, Belur Shivappa Gnanesh; Ali, Aamer Mousa

doi:10.4103/jlp.jlp_160_17

Cited by 14 publications

(6 citation statements)

References 23 publications

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“…When using microbial metabolomics to identify Streptococcus mutans and Actinomyces viscosus, the researchers found that PCA could classify and identify the two species of bacteria, and the one-dimensional spectrum time of magnetic resonance samples was only 5 min, which was convenient and fast (Kunze et al, 2010;Ribeiro et al, 2017;Salman et al, 2018). The spectra of extracellular metabolites of Actinomyces tunica, Actinomyces neisseriae, Streptococcus sanguis, Streptococcus cousins, Porphyromonas gingivalis, Prevotella intermedia, Clostridium nucleatum, Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, and Candida in a stable phase were compared by the same method (Hetrodt et al, 2018;Sanz et al, 2017).…”

Section: Oral Floramentioning

confidence: 99%

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Meng¹,

LI²,

YANG³

et al. 2023

Biocell

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In recent years, microbial metabolomics, a new field that has attracted wide attention, provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts. Many microorganisms are found in the human intestine, oral cavity, vagina, etc. Probiotics could maintain the good health of the host, while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind.Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system, which could provide key information to understand the related metabolic pathways and associated changes. This approach analyzes the final products of cellular regulatory processes, the level of which can be regarded as the ultimate response of the biological system to genetic or environmental changes. Microbial metabolomics has been widely used in different research fields, such as microbial phenotypic classification, mutant screening, metabolic pathways, microbial metabolic engineering, fermentation engineering monitoring and optimization, microbial environmental pollution, and so on. However, there are only a few reviews on microbial metabolomics of probiotics and pathogens. This review summarizes the main methodologies, including sample preparation, identification of metabolites, data processing, and analysis. Recent applications in microbial metabolomics of probiotics and pathogens are also described. This paper first summarized the research progress and application of microbial metabolomics from two aspects: probiotics and pathogenic bacteria. Probiotics and pathogenic bacteria do not exist independently most of the time; hence, these were reviewed in the research field of coexistence of probiotics and pathogenic bacteria, which was subdivided into important microbial research fields closely related to human health, including the human gut, oral cavity, food, and nutrition-related microorganisms. Then, the main problems and trends associated with microbial metabolomics are discussed.

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Section: Oral Floramentioning

confidence: 99%

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Meng¹,

LI²,

YANG³

et al. 2023

Biocell

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“…The plant has been studied for its major components, mainly coumarins, terpenoids, alkaloids, flavonoids, aliphatic acids, ketones, rutarin, isorutarin, rutin, isorhamnetin-3-O-rutinoside, cnidioside A, methylcnidioside A, cnidioside B, methyl ester [3], caffeic acid, chlorogenic acid, cinnamic acid, p-coumaric acid and protocatechuic acid [4], ɣ-fagarine and kokusaginine [5], limonene, thymol and carvacrol [6]. The richness of Ruta in active molecules may explain its great effectiveness in therapeutic treatments and its use in folk medicine, mainly as an antispasmodic, anticancer, treatment of menstrual disorder, an abortifacient, a sedative, antiinflammatory, antipyretic, anti-microbial, etc.…”

Section: Introductionmentioning

confidence: 99%

"Evaluation of the Phenolic Contents, Antioxidant and Antimicrobial Activities of Leaves Extracts of Ruta Graveolens"

Halouani¹,

Abdelli²,

Slama³

et al. 2021

BJSTR

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Ruta graveolens, better known as rue, is a polyvalent herb rich in secondary metabolites that has demonstrated different biological properties and its various medicinal properties. Ruta belongs to the Rutaceae family and is native to the Mediterranean region. We choose same species plant, from two origin, North Africa (Tunisia) and South Europe (France). Different extracts were obtained from dried leaves by using five solvents with increasing polarity, petroleum ether, chloroform, ethyl acetate, ethanol, methanol, and water. Different extracts from both plants, demonstrated in most of tests the same profile. Starting with yield extraction evaluation, a high yields were obtained with ethanol, methanol and aqueous plant extracts. Phenols content assessment reveals an important amount in ethanolic and methanolic extracts of polyphenols, flavonoids and in petroleum ether, chloroform and ethyl acetate of tannins. The antioxidant power was evaluated by using four methods, mainly DPPH, ABTS, β-Carotene and FRAP. Results are expressed as Inhibitory Concentration 50 and the best performance was noted with ethanolic and methanolic extracts.Effect of five organic and aqueous extracts of both plants on growth of two Gramnegative and two Gram-positive bacteria and Candida albicans was evaluated by MIC and MBC determination. Ethanol and methanol extracts of both Ruta exerts a bactericidal effect on different bacterial strain. While, a selective antifungal activity was noted only with methanolic extracts. Results were confirmed with MBC/MIC ratio (less than or equal to 4). Phenols content, especially polyphenols and flavonoids, showed good correlation with antioxidant and antimicrobial activities, which confirm its significant contribution to those activities of Ruta graveolens extracts.

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“…Truxillense, a los que se les puede relacionar sus propiedades antibacterianas. (17,18,19,24,25) Loyola y otros, (17) reportaron el efecto antibacteriano del extracto etanólico de E. coca Lam en las concentraciones de 50 % y 75 % sobre S. mutans, formando halos de inhibición a las 24 horas de 10,1 ± 0,7 mm y 11,2 ± 0,7, respectivamente; incrementándose el efecto a las 48 horas con halos inhibitorios de 10,5 ± 0,6 mm y 11,3 ± 0,7 mm en cada concentración. Estos resultados son semejantes a los obtenidos en la presente investigación y los comunicados por Salcedo, (24) donde las concentraciones de 50% y 100% de E. coca novogranatense var.…”

Section: Discussionunclassified

“…La población, estuvo constituida por placas petri sembradas con cepas estandarizadas de S. mutans ATCC ® 35668 ™ y L. acidophilus ATCC ® 4356 ™ , obtenidas de la American Type Culture Collection (ATCC), importadas por la empresa Genlab S.A.C. Para la muestra se tomó como referencia investigaciones previas, (18,19) y estuvo conformada por 20 placas petri por cada concentración y tipo de extracto; resultaron 160 unidades experimentales. (20,21)…”

Section: Métodosunclassified

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Comparación del efecto antibacteriano del extracto acuoso y alcohólico de hojas secas de Erythroxylum coca "Coca" frente a Streptococcus mutans ATCC®35668™ y Lactobacillus acidophilus ATCC®4356™

Alva¹

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Introducción: La hoja de Erythroxylum coca, se utiliza tradicionalmente en diversos países por sus bondades farmacológicas. Objetivo: Comparar el efecto antibacteriano del extracto acuoso y alcohólico de hojas de Erythroxylum coca frente a Streptococcus mutans ATCC®35668™ y Lactobacillus acidophilus ATCC®4356™. Métodos: Estudio experimental in vitro. Se obtuvo el extracto acuoso y alcohólico de E. coca. La muestra estuvo conformada por 20 placas petri para cada concentración y extracto evaluado. La media de los diámetros de los halos de inhibición, se expresaron en milímetros y se analizaron mediante la prueba de Kruskal-Wallis y el análisis post hoc de Dunn con ajuste de Bonferroni. La comparación de los efectos entre las 24 y 48 horas se realizó mediante el test Wilcoxon con un nivel de significancia del 5 %. Resultados: La concentración alcohólica de 25 mg/mL fue más efectiva sobre S. mutans, al compararla con los otros grupos (p< 0,05). En el extracto alcohólico se observó diferencias con la concentración de 50 mg/mL a las 24 y 48 horas frente a S. mutans (p< 0,05). Se observaron diferencias significativas al comparar el efecto inhibitorio del extracto acuoso de 75 mg/mL y 50 mg/mL a las 24 y 48 horas frente a L. acidophilus (p< 0,05) y para el extracto alcohólico las diferencias fueron estadísticamente significativas con las concentraciones de 50 mg/mL y 25 mg/mL (p< 0,05). Conclusión: Existen diferencias en el efecto antibacteriano del extracto acuoso y alcohólico de las hojas de E. coca frente a S. mutans y L. acidophilus.

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Determination of antibacterial activity and metabolite profile of Ruta graveolens against Streptococcus mutans and Streptococcus sobrinus

Cited by 14 publications

References 23 publications

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

"Evaluation of the Phenolic Contents, Antioxidant and Antimicrobial Activities of Leaves Extracts of Ruta Graveolens"

Comparación del efecto antibacteriano del extracto acuoso y alcohólico de hojas secas de Erythroxylum coca "Coca" frente a Streptococcus mutans ATCC®35668™ y Lactobacillus acidophilus ATCC®4356™

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