Determination of antibiotic residues in Canadian slaughter animals by thin-layer chromatography-bioautography

Salisbury, C. D. C.; Rigby, C E; Chan, Wayne

doi:10.1021/jf00085a024

Cited by 29 publications

(7 citation statements)

References 6 publications

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“…Furthermore, Horie et al [13] recorded 50 ng g -1 as the detection limit for each antibiotic of these macrolides. In addition, the results were approximately in accordance with those of Salisbury et al [18] and Okerman et al [19] who reported detection limits of 1 and 10 ng g -1 for ERY and TYL, respectively, in the spiked meat samples using M. leuteus ATCC 9341 as test organism. This increased sensitivity was due to increasing the sample weight, high recoveries and dissolving the dried extract in a small volume of methanol (100 μL), attributing to increase the concentration of antibiotic in the loaded methanolic extract onto the TLC.…”

Section: Sensitivitysupporting

confidence: 90%

Determination of tylosin, spiramycin, and erythromycin residues in egyptian buffaloes’ meat by thin-layer chromatography-bioautography

Ahmed

Sree²,

Abdel-Fattah³

et al. 2013

Journal of Planar Chromatography – Modern TLC

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Antibiotic extraction from solid matrices is generally complicated unlike aqueous phases. Consequently, increasing the extraction efficiency of antibiotics from solid matrices has received a great concern. Therefore, the main objective of this study was to determine three macrolide antibiotics; tylosin (TYL), spiramycin (SPIR), and erythromycin (ERY) in buffaloes' meat using the recommended method of thin-layer chromatography-bioautography (TLC-B) after some modifications. Antibiotics were extracted by 0.2% metaphosphoric acid-methanol (6:4, v/v), and then the extracts were cleaned up using an Oasis HLB cartridges (200 mg). The recovery ratios at 0.5, 1, and 2 maximum residue limits (MRLs) with high precision were 84.19-92.22%, 83.09-89.42%, and 84.89-90.28%, respectively. The detection limits were 12, 45, and 2 ng g -1 for TYL, SPIR, and ERY, respectively. Forty-five samples of Egyptian buffaloes' meat were analyzed using the adapted method. Only 9 and 5 samples out of the 45 samples were positive for TYL and ERY, respectively, with mean concentrations of 42.59 and 31.21 (ng g -1 ) which were lower than the permitted MRLs recommended by the European community.

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Section: Sensitivitysupporting

confidence: 90%

Determination of tylosin, spiramycin, and erythromycin residues in egyptian buffaloes’ meat by thin-layer chromatography-bioautography

Ahmed

Sree²,

Abdel-Fattah³

et al. 2013

Journal of Planar Chromatography – Modern TLC

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“…Similarly, sample #3 was classified as penicillin (+) by the microbial assay, whereas LC/MS n gave a negative result. These discrepancies between the two techniques could be due to a variety of causes, including poor detection of DCCD at lower concentrations, the presence of non‐ β ‐lactam antibiotics, or the presence of natural microbial inhibitors 22–25…”

Section: Resultsmentioning

confidence: 99%

Confirmatory analysis of β‐lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry

Fagerquist

Lightfield

2003

Rapid Comm Mass Spectrometry

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Eleven beta-lactam antibiotics were analyzed in fortified and incurred beef kidney tissue using high-performance liquid chromatography/electrospray ionization/selective reaction monitoring-ion trap tandem mass spectrometry (LC/ESI-SRM-MS(n)). The analytes included: deacetylcephapirin, amoxicillin, cephapirin, desfuroylceftiofur cysteine disulfide (DCCD, a biomarker of ceftiofur), ampicillin, cefazolin, Pen G, oxacillin, cloxacillin, naficillin and dicloxicillin. Analytes were extracted with acetonitrile and water. Clean-up was performed by solid-phase extraction. Limits of confirmation in fortified tissue are as follows (tolerances or target levels in parentheses): deacetylcephapirin: 10-50 ng/g (100 ng/g); amoxacillin: 50-100 ng/g (10 ng/g); cephapirin: 10 ng/g (100 ng/g); DCCD: 500 ng/g (8000 ng/g); ampicillin: 10 ng/g (10 ng/g); cefazolin: 10 ng/g (10-50 ng/g); Pen G: 10 ng/g (50 ng/g); oxacillin: 10 ng/g (10-50 ng/g); cloxacillin: 10 ng/g (10 ng/g); naficillin: 10 ng/g (10-50 ng/g); dicloxacillin: 100-500 ng/g (10-50 ng/g). The present method was also tested on incurred kidney tissue that had previously been analyzed using a microbial assay. Good correspondence was found between the results from this new method and the bioassay. However, the present method is much more specific and, in several cases, more sensitive than the bioassay. In addition, the time of analysis is significantly shorter than the bioassay. We also found that SRM MS(n) was superior in the analysis of unknown incurred tissue than full spectrum MS(n). We also obtained an MS/MS spectrum of DCCD that is significantly at variance with previously published fragmentation spectra.

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“…Identification of residues was performed using a modified version of the thin-layer chromatography-bioautography of Salisbury et al (1989). To increase the sensitivity of the procedure to virginiamycin, Micrococcus luteus replaced Bacillus subtilis as the test organism.…”

Section: Source Of Variationmentioning

confidence: 99%

The Response of Male Chicken Broilers to the Dietary Addition of Virginiamycin

et al. 1990

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Two replicate trials, each involving 400 Arbor Acre male broiler chicks, were conducted to determine the effect of virginiamycin as a growth promoter when added to either the feed or drinking water. A control group received no growth promoter while one treatment group was provided a diet containing 11 mg of virginiamycin/kg. Another treatment group was provided drinking water containing virginiamycin in amounts calculated to ensure equivalent or one-half equivalent intake of the antibiotic. Virginiamycin supplementation had no significant (P greater than .05) effect on mortality or feed conversion ratios, regardless of the mode of administration. Body weights at 21 days of age but not at 42 days of age were significantly (P less than .05) heavier for broilers receiving virginiamycin via the drinking water. The inclusion of virginiamycin in the feed failed to improve body weights at either 21 or 42 days of age.

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Determination of antibiotic residues in Canadian slaughter animals by thin-layer chromatography-bioautography

Cited by 29 publications

References 6 publications

Determination of tylosin, spiramycin, and erythromycin residues in egyptian buffaloes’ meat by thin-layer chromatography-bioautography

Determination of tylosin, spiramycin, and erythromycin residues in egyptian buffaloes’ meat by thin-layer chromatography-bioautography

Confirmatory analysis of β‐lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry

The Response of Male Chicken Broilers to the Dietary Addition of Virginiamycin

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