Determination of Antibiotic Sensitivity of Bacteroid fragilis Isolated from Patients and Healthy Individuals in Imam Reza Center of Medical Teaching and Treatment-Tabriz

Abstract

Background:Bacteroides fragilis are among the most important anaerobic bacteria behind most of the anaerobic infections. They have acquired resistance to essential treatment antibiotics of anaerobic infections, more than other anaerobic bacteria. Objectives: The goal of this study is to determine the resistance of isolated B. fragilis against common anaerobic infections treatment antibiotics. Patients and Methods: A total of 188 fecal samples including 59 samples from hospitalized patients, 84 samples from outpatients, and 45 samples from healthy individuals were collected. The samples were cultured in Bacteroides-Bile-Esculine agar and KanamycinVancomycin-Laked Blood media and were incubated in anaerobic atmosphere at 37°C for at least 48 hours. Suspected one millimeter sized colonies with black surroundings, were selected and further studied using MID8, as well as biochemical tests. For MIC determination of antibiotics against isolated B. fragilis, Etest was used. Results: There wasn't any difference between antibiotic resistance patterns of isolated B. fragilis from hospitalized patients or outpatients, including diarrheal and non-diarrheal cases, and resistant pattern of isolates from healthy individuals. All or most of isolated B. fragilis were susceptible to imipenem (100%), metronidazole (95%), rifampin (100%) and piperacillin/tazobactam (95%). On the contrary, they exhibited resistance to other antibiotics such as clindamycin (90%), and chloramphenicol (55%). Conclusions:In the present study, we have figured out that a number of the important anaerobic infections treatment antibiotics have lost partly or totally their effectiveness on B. fragilis.

Role of Enterotoxigenic Bacteroides fragilis in Children Less Than 5 Years of Age With Diarrhea in Tabriz, Iran

“…These results are similar to others study carried by Nakano et al ( 16 ). In contrast to the results obtained in our study, Akhi et al reported 5% resistance to metronidazole among B. fragilis isolates ( 7 ). In other previous studies, metronidazole resistant Bacteroides spp.…”

“…Bacteroides fragilis is an anaerobic Gram-negative bacillus and a member of the normal flora community of the human gastrointestinal tract and vagina. B. fragilis represents a common cause of endogenous infections in humans that is frequently associated with polymicrobial infections such as intra-abdominal, diabetic foot, obstetric-gynecologic tract and surgical site infections, as well as toxin-dependent diarrhea in adults and children ( 7 , 8 ). Enterotoxigenic Bacteroides fragilis (ETBF) induced diarrhea in children has been reported by different researchers in various parts of the world, but ETBF has also been found in non-diarrheal fecal samples ( 9 , 10 ).…”

Bacterial etiology and antibiotic susceptibility pattern of diabetic foot infections in Tabriz, Iran

“…For enrichment and isolation of Clostridium perfringens , a drop of syringe specimen was introduced in cooked meat broth media and incubated at 45ºC for 4–6 h. Thereafter, one loop of this incubated media was sub-cultured in sheep blood agar plate and incubated under anaerobic condition and examined after 24 and 48 h [ 15 ], [ 17 ]. For identification of Gram-negative anaerobic bacteria, biochemical test such as reaction in Bile Esculin agar and MAST ID MID8 ANAEROB ID RING (MAST CO) was used [ 18 ].…”

Emerging Metronidazole Resistance in Anaerobes and Mapping Their Susceptibility Behaviour

“…The infections caused by anaerobic bacteria are some of the most important causes of morbidly and mortality in developing countries (Akhi et al, 2013). The development of resistance to metronidazole leading to treatment failure during the course of metronidazole therapy in a patient has been reported (Buta et al, 2010;Soki et al, 2013).…”