2022
DOI: 10.1007/s11274-022-03332-2
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Determination of antibiotic susceptibility of bacteria by flow cytometric method

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Cited by 6 publications
(6 citation statements)
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References 23 publications
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“…For the routine use of FCM in the detection of bacterial contamination in PS, it would be appropriate to take a sample with a satellite bag after the PS is taken and leave it to incubate at 37 °C for 5 days like a blood culture bottle. In line with the data we obtained in our other study (Hatipoglu et al 2022 ) and as suggested by some researchers, in cases where there is no satellite bag, we think that pre-incubation of the sample taken from the PS for 1–2 h in an incubator at 37 °C would be useful in detecting the presence of bacteria with FCM. We recommend that researchers consider this process in their study design.…”
Section: Discussionsupporting
confidence: 92%
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“…For the routine use of FCM in the detection of bacterial contamination in PS, it would be appropriate to take a sample with a satellite bag after the PS is taken and leave it to incubate at 37 °C for 5 days like a blood culture bottle. In line with the data we obtained in our other study (Hatipoglu et al 2022 ) and as suggested by some researchers, in cases where there is no satellite bag, we think that pre-incubation of the sample taken from the PS for 1–2 h in an incubator at 37 °C would be useful in detecting the presence of bacteria with FCM. We recommend that researchers consider this process in their study design.…”
Section: Discussionsupporting
confidence: 92%
“…For bacteriological analyzes, we used the FCM analysis protocol that we used in our previous study (Hatipoglu et al 2022 ). As described in detail above, 1 ml was taken from all samples (spiked and non-spiked APSs), platelets were lysed, centrifuged, pellet suspension was prepared, fluorescent staining was performed and analyzed in a flow cytometry device (Accuri C6, Becton Dickinson, USA).…”
Section: Methodsmentioning
confidence: 99%
“…FCM assay also offers an advantage in higher flexibility compared to current automated AST tools, such as Vitek-2 [22,36]. High correlations (>90%) of flow cytometric determination of AST against Vitek-2 was documented across multiple species [22]. These flexibilities include testing any bacteria species against any antibiotics, including antibiotics combinations [37][38][39].…”
Section: Discussionmentioning
confidence: 99%
“…One major advantage of FCM is the rapid assessments of cells at the single cell resolution. FCM assay also offers an advantage in higher flexibility compared to current automated AST tools, such as Vitek-2 [ 22 , 36 ]. High correlations (>90%) of flow cytometric determination of AST against Vitek-2 was documented across multiple species [ 22 ].…”
Section: Discussionmentioning
confidence: 99%
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