Determination of antibiotics and detergent residues in decellularized tissue-engineered heart valves using LC–MS/MS

Kraft, Letícia; Ribeiro, Victoria Stadler Tasca; Wollmann, Luciana Cristina; Suss, Paula Hansen; Tuon, Felipe Francisco

doi:10.1007/s10561-020-09856-x

Cited by 13 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Binding to collagen and elastin fibers, SDS can damage ECM architecture ( Crapo et al, 2011 ; Keane et al, 2015 ), influence cell development ( Rieder et al, 2004 ; Gratzer et al, 2006 ; Cebotari et al, 2010 ; Reing et al, 2010 ; Andrée et al, 2014 ; Zvarova et al, 2016 ; Alizadeh et al, 2019 ; Kraft et al, 2020 ), and promote in vivo host adverse reactions ( Friedrich et al, 2018 ). Combining DIW and PBS bath steps, the residual SDS in the produced scaffolds was below different levels considered non-cytotoxic and similar to those found by previous research groups ( Gratzer et al, 2006 ; Cebotari et al, 2010 ; Zvarova et al, 2016 ; Roderjan et al, 2019 ; Kraft et al, 2020 ). Associated with the increasing proliferation of the seeded fibroblasts, these results confirmed the non-toxicity of d-NAC scaffolds and their ability to promote cell adherence and proliferation.…”

Section: Discussionmentioning

confidence: 99%

Reconstruction of the human nipple–areolar complex: a tissue engineering approach

Maistriaux,

Foulon,

Fievé

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Introduction: Nipple–areolar complex (NAC) reconstruction after breast cancer surgery is challenging and does not always provide optimal long-term esthetic results. Therefore, generating a NAC using tissue engineering techniques, such as a decellularization–recellularization process, is an alternative option to recreate a specific 3D NAC morphological unit, which is then covered with an in vitro regenerated epidermis and, thereafter, skin-grafted on the reconstructed breast.Materials and methods: Human NACs were harvested from cadaveric donors and decellularized using sequential detergent baths. Cellular clearance and extracellular matrix (ECM) preservation were analyzed by histology, as well as by DNA, ECM proteins, growth factors, and residual sodium dodecyl sulfate (SDS) quantification. In vivo biocompatibility was evaluated 30 days after the subcutaneous implantation of native and decellularized human NACs in rats. In vitro scaffold cytocompatibility was assessed by static seeding of human fibroblasts on their hypodermal side for 7 days, while human keratinocytes were seeded on the scaffold epidermal side for 10 days by using the reconstructed human epidermis (RHE) technique to investigate the regeneration of a new epidermis.Results: The decellularized NAC showed a preserved 3D morphology and appeared white. After decellularization, a DNA reduction of 98.3% and the absence of nuclear and HLA staining in histological sections confirmed complete cellular clearance. The ECM architecture and main ECM proteins were preserved, associated with the detection and decrease in growth factors, while a very low amount of residual SDS was detected after decellularization. The decellularized scaffolds were in vivo biocompatible, fully revascularized, and did not induce the production of rat anti-human antibodies after 30 days of subcutaneous implantation. Scaffold in vitro cytocompatibility was confirmed by the increasing proliferation of seeded human fibroblasts during 7 days of culture, associated with a high number of living cells and a similar viability compared to the control cells after 7 days of static culture. Moreover, the RHE technique allowed us to recreate a keratinized pluristratified epithelium after 10 days of culture.Conclusion: Tissue engineering allowed us to create an acellular and biocompatible NAC with a preserved morphology, microarchitecture, and matrix proteins while maintaining their cell growth potential and ability to regenerate the skin epidermis. Thus, tissue engineering could provide a novel alternative to personalized and natural NAC reconstruction.

show abstract

Section: Discussionmentioning

confidence: 99%

Reconstruction of the human nipple–areolar complex: a tissue engineering approach

Maistriaux,

Foulon,

Fievé

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Regarding biocompatibility and biosecurity, this study conducted in vitro tests, including cell affinity, DNA released in culture medium [ 45 ] and CCK-8 assay [ 46 ], as well as in vivo tests, including inflammatory factor ELISA and neutrophil immunohistochemical staining [ 47 ]. The possible reasons for immune rejection and cytotoxicity of decellularized ECM materials mainly include residual cell components and toxic substances remaining in the process of decellularization and sterilization [ 20 , 48 , 49 ]. The results confirmed that CDCC and SDCC had excellent cell compatibility and did not cause severe acute systemic and local immune responses.…”

Section: Discussionmentioning

confidence: 99%

Fabrication and evaluation of an optimized xenogenic decellularized costal cartilage graft: preclinical studies of a novel biocompatible prosthesis for rhinoplasty

Lin

Tao

et al. 2021

Regenerative Biomaterials

View full text Add to dashboard Cite

On account of the poor biocompatibility of synthetic prosthesis, millions of rhinoplasty recipients have been forced to choose autologous costal cartilage as grafts, which suffer from limited availability, morbidity at donor site and prolonged operation time. Here, as a promising alternative to autologous costal cartilage, we developed a novel xenogeneic costal cartilage and explored its feasibility as a rhinoplasty graft for the first time. Adopting an improved decellularization protocol, in which the ionic detergent was substituted by trypsin, the resulting decellularized graft was confirmed to preserve more structural components and better mechanics, and eliminate cellular components effectively. The in vitro and in vivo compatibility experiments demonstrated that the decellularized graft showed excellent biocompatibility and biosecurity. Additionally, the functionality assessment of rhinoplasty was performed in a rabbit model, and the condition of grafts after implantation was comprehensively evaluated. The optimized graft exhibited better capacity to reduce the degradation rate and maintain the morphology, in comparison to the decellularized costal cartilage prepared by conventional protocol. These findings indicate that this optimized graft derived from decellularized xenogeneic costal cartilage provides a new prospective for future investigations of rhinoplasty prosthesis and has great potential for clinical application.

show abstract

“…Persistent positive cultures after decontamination were excluded [32]. It has been observed that there is not enough antibiotic present in the processed pericardium to inhibit bacterial growth [6]. Each step of the process is depicted in the Figure 9.…”

Section: Tissue Preparation and Decontaminationmentioning

confidence: 99%

“…The use of antibiotics in decellularized tissues, like decellularized pericardium, is a mean to reduce the risk of infection and promote healing after implantation [5]. The decellularization process removes all cells and DNA, leaving behind extracellular matrix scaffold that can be impregnated with antibiotics [6]. The antibiotics can be incorporated into the tissue before, during, or after decellularization [7].…”

Section: Introductionmentioning

confidence: 99%

Biological Membrane with Antimicrobial Activity Based on Lyophilized and Decellularized Bovine Pericardium Impregnated with Vancomycin or Silver Nanoparticles

Collatusso,

Dantas,

Suss

et al. 2023

Preprint

View full text Add to dashboard Cite

Introduction: Infections associated with medical implants pose significant challenges to healthcare, demanding the development of new effective antimicrobial strategies. This study focuses on the creation of a novel biological membrane with enhanced antimicrobial properties by with the incorporation of either vancomycin or silver nanoparticles. Methods: Pericardium samples were impregnated with either silver nanoparticles (AgNPs) or vancomycin (VAN). The antimicrobial activity of the membranes was assessed through qualitative microbiological analysis, biofilm production and animal in vivo model of infection. Histological analysis was performed on the pericardium samples, and quantitative microbiological analysis was conducted on the tissue from the animal model and biocompatibility evaluation. Statistical analyses were carried out using appropriate tests. Results: Microbiological tests confirmed both VAN and AgNP showed inhibition halos against bacteria. Histological analysis indicated minimal inflammatory response and bacterial presence in VAN and AgNP groups compared to the control group revealed reduced bacterial presence. Biomechanical testing showed that polypropylene had the lowest tensile strength, while AgNP-impregnated pericardium exhibited lower resistance than VAN-impregnated and control pericardium. Conclusion: Lyophilized and decellularized bovine pericardium as a substrate, combined with the incorporation of vancomycin or silver nanoparticles, provides a versatile platform for combating infections associated with medical implants.

show abstract

Determination of antibiotics and detergent residues in decellularized tissue-engineered heart valves using LC–MS/MS

Cited by 13 publications

References 20 publications

Reconstruction of the human nipple–areolar complex: a tissue engineering approach

Reconstruction of the human nipple–areolar complex: a tissue engineering approach

Fabrication and evaluation of an optimized xenogenic decellularized costal cartilage graft: preclinical studies of a novel biocompatible prosthesis for rhinoplasty

Biological Membrane with Antimicrobial Activity Based on Lyophilized and Decellularized Bovine Pericardium Impregnated with Vancomycin or Silver Nanoparticles

Contact Info

Product

Resources

About