Determination of Antifungals in Rodent Diet by Supercritical Fluid Extraction Followed by Packed Column Supercritical Fluid Chromatography with Ultraviolet Detection

Abstract

Supercritical fluid extraction (SFE) followed by packed column supercritical fluid chromatography with ultraviolet detection was evaluated as a quantitative method for determining 4 antifungals (fluconazole, tioconazole, hexaconazole, and UK- 47,265) in rodent diet. Chromatography was achieved with a cyano-bonded silica column, UV detection at 210 nm, and methanol-modified supercritical carbon dioxide as mobile phase. The effects of modifier concentration, temperature, and column pressure on antifungal retention time was studied. Off-line SFE was optimized at 2 spike levels, ranging from 0.5 to 10 g/kg, for each of the 4 antifungals. Average recoveries ranged from 79.0% for UK-47,265 to 96.5% for hexaconazole. Overall, the procedure provides a suitable method for analyzing antifungals in spiked rodent diet.

Fluidos supercríticos em química analítica. III.: aplicações

“…Este é um exemplo típico de porque a SFC é amplamente usada na análise de drogas, pois os fármacos têm se constituído como um verdadeiro nicho de aplicação para SFC 59 . Por ex,, agentes antifúngicos [60][61][62] , sulfato de salbutamol 63 , benzodiazepinas 64 , sulfonamidas 65 , tocoferóis 66 e esteróides 67,68 foram determinados com sucesso em uma variedade ampla de matrizes.…”

Supercritical Fluid Chromatography and Extraction

“…Analysis time in pcSFC/ELSD was approximately one-third that in otSFC/FID. Other drugs determined by pcSFC include antifungal agents (194), salbutamol sulfate and related compounds (195), paclitaxel and related taxanes (196), and benzodiazepines (197). Most of these works included investigations of various columns, mobilephase modifiers and additives, and chromatographic conditions, to provide a rapid, high-resolution separation.…”