Determination of Antimicrobial Activity of Sorrel (Hibiscus sabdariffa) onEsherichia coliO157:H7 Isolated from Food, Veterinary, and Clinical Samples

Abstract

The use of medicinal plants as natural antimicrobial agents is gaining popularity. Sorrel (Hibiscus sabdariffa) is widely used for the treatment of diseases. The objective of this study was to investigate the antimicrobial activity of sorrel on Escherichia coli O157:H7 isolates from food, veterinary, and clinical samples. Phenolics of the calyces were extracted from 10 g of ground, freeze-dried samples using 100 mL of 80% aqueous methanol. Concentrations of 10%, 5%, and 2.5% methanol extract of sorrel were investigated for its antimicrobial activity. Inhibition zones were indicated by a lack of microbial growth due to inhibitory concentrations of sorrel diffused into semisolid culture medium beneath the sorrelimpregnated disk. The results of this experiment showed that the most potent sorrel concentration was 10%, then 5%, and finally 2.5%. The overall mean zone of inhibition for the sorrel extract was 12.66 mm for 10%, 10.75 mm for 5%, and 8.9 mm for 2.5%. The highest inhibition zones (11.16 mm) were observed in veterinary samples, and the lowest (10.57 mm) in the food samples. There were significant (P < .05) differences among mean zones of inhibition found in the food, veterinary, and clinical sources. Based on the source of samples and concentration of sorrel extract, the lowest mean inhibition was 7.00 -0.04 mm from clinical samples, and the highest was 15.37 -0.61 mm from a food source. These findings indicated that sorrel was effective at all levels in inhibiting E. coli O157:H7; thus it possesses antimicrobial activity and hold great promise as an antimicrobial agent.

Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa

“…The antimicrobial activity of H. sabdariffa has been reported against foodborne and food spoilage microorganisms, like E. coli O157:H7, is a major foodborne pathogen [23,37], also against Gram-negative bacteria such as Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus vulgaris, Salmonella enterica and some multidrug resistant Salmonella strains, in addition to some Gram-positive bacteria such as Bacillus subtilis, S. aureus, Staphylococcus epidermis, and Staphylococcus cereus [24,25,37,38].…”

“…Finely powdered and extracted in a Soxhlet with 2 L of methanol for 48 h. The methanol was evaporated by vacuum pressure, the ×10 concentrated extract 10 mg/ml was used in further experiments [23,25,31].…”

“…The antimicrobial efficiency of H. sabdariffa L. against E. coli and some other Gram-negative bacteria has been demonstrated [23][24][25]. Combination of methanol extracts of H. sabdariffa L. with antibiotics enhances the activity of some antibiotics against resistant strain and standard strain of E. coli [26].…”

Roselle Calyx Ethanol Extract Stimulates Oral Mucosal Wound Healing

“…This process is performed by activating fibroblast that has similar properties to smooth muscle cells, enabling necessary contraction and tension for damage repair. Growth factors are of great importance in the migration, proliferation, and differentiation of epithelial cells as well as in the formation of connective tissue and the growth of new vessels during the healing process [2,16,19].…”

“…Former studies also reported that surgical wounds developed keloid tissue (collagen scarring) or led to extended coagulation necrosis in all tissue layers of the oral cavity [6,14,19]. Former in vitro study reported that roselle extract showed the novel property as potential supplement and gene protectant against oxidative damage to culture hematopoietic stem cells.…”