Determination of antimony species with fluoride as modifier and flow injection hydride generation inductively-coupled plasma emission spectrometry

Ulrich, Nina

doi:10.1016/s0003-2670(00)00939-9

Cited by 32 publications

(17 citation statements)

References 17 publications

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“…First, total intracellular antimony content was measured with FI-ICP-MS (25,26), and the results are summarized under "Total [Sb] i " in Tables 1 and 2. The second method used to determine the content of antimony hydride (SbH 3 ) was FI-HG-ICP-MS (27,28). In this method, Sb V and Sb III in cell extracts were separately reduced to SbH 3 , which then flowed through the plasma to the mass spectrometer, where its concentration was subsequently determined (Tables 1 and 2 With a similar set of experiments using WT and mutant amastigotes, the data in Table 2 1 The abbreviations used are: FI-ICP-MS, flow injection-inductively coupled plasma mass-spectrometry; FI-HG-ICP-MS, flow injectionhydride generation-inductively coupled plasma-mass spectrometry; IC-ICP-MS, ion-chromatography-inductively coupled plasma-massspectrometry; WT, wild type; HPLC, high performance liquid chromatography; LD, lethal dose.…”

Section: Resultsmentioning

confidence: 99%

“…4) suggest the possibility of covalent interactions of either Sb V or Sb III with intracellular molecules. The bound antimony may not be reduced by BH 4 (27), thus explaining the differences between total [Sb] i and the reducible intracellular Sb V and Sb III measured in Tables 1 and 2.…”

Section: Figmentioning

confidence: 90%

“…Determination of Sb III and Sb V -Intracellular Sb III and Sb V were measured using flow injection-hydride generation-inductively coupled plasma-mass spectrometry (FI-HG-ICP-MS), which has recently been developed (27). Briefly, Sb III was reduced to antimony hydride (SbH 3 ) using 0.2% sodium tetrahydridoborate in de-ionized water and was directly quantified using mass spectrometry at m/z 121.…”

Section: Determination Of Intracellular Antimonymentioning

confidence: 99%

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Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani

Shaked‐Mishan¹,

Ulrich²,

Ephros³

et al. 2001

Journal of Biological Chemistry

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208

158

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The standard treatment of human visceral leishmaniasis involves the use of pentavalent antimony (Sb V ). Its mechanism of action is unknown because of the limited information available about intracellular antimony metabolism and about the genes that regulate these processes. Herein, flow injection-inductively coupled plasma mass spectrometry (ICP-MS), flow injection hydride generation ICP-MS, and ion chromatography ICP-MS were used to measure antimony accumulation and intracellular metabolism in the human protozoan parasite Leishmania donovani. V reduction has yet to be identified, and it may or may not be enzymatic. This is the first description of intracellular Sb V reducing activity in Leishmania as well as in any prokaryotic or eukaryotic cell.Leishmania donovani is the major causative agent of visceral leishmaniasis. In nature, the parasite exists either as an extracellular promastigote found in the alimentary tract of sandflies or as an obligatory intracellular amastigote found in phagolysosomes of mammalian macrophages(1-3). During the last several years, a number of laboratories have utilized axenic culture of L. donovani amastigotes for the direct evaluation of cell biological and biochemical processes in the amastigote, devoid of the host macrophage (4 -7,8,9,10). This technique has also been used to investigate the mechanism of drug action and resistance as well as for screening of potential new drugs(11-13). The treatment of choice of human visceral leishmaniasis is the administration of pentavalent antimony (Sb V )-containing drugs such as sodium stibogluconate (Pentostam, Wellcome, Beckenham, UK) or meglumine antimoniate (Glucantime, Rhone-Poulenc, Paris, France). Despite extensive use of these compounds over the last decades, the mechanism of action remains unclear.It has been hypothesized that Sb V is not toxic to Leishmania, but rather that it is enzymatically reduced, probably by the host macrophage, to Sb III , the form of antimony that is highly Leishmania-toxic (14 -18). In contrast, it has been shown that Sb V is directly toxic to axenic amastigotes (12,13,19), thus negating the necessity of the macrophage for Sb V reduction. Furthermore, these data imply that either the parasite reduces Sb V to Sb III intracellularly, and thus Sb III is directly toxic to amastigotes, or that both oxidation states of antimony are active against Leishmania amastigotes. The modes of action of the two oxidation states of antimony (Sb III and Sb V ) on Leishmania are, as yet, not fully understood.Several groups have shown obligatory cross-resistance between Sb V , Sb III , and arsenite (As III ) in Leishmania tarentolae, Leishmania major, Leishmania mexicana, L. donovani and Leishmania infantum (11,[20][21][22]. In contrast, it has been suggested that, at least in L. donovani, such cross-resistance does not necessarily occur (12). Furthermore, it has been demonstrated that, when bound to organic compounds, structural similarities exist between Sb III and Sb V (23). For example, the trivalent antimony comp...

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Section: Resultsmentioning

confidence: 99%

Section: Figmentioning

confidence: 90%

Section: Determination Of Intracellular Antimonymentioning

confidence: 99%

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Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani

Shaked‐Mishan¹,

Ulrich²,

Ephros³

et al. 2001

Journal of Biological Chemistry

Self Cite

208

158

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“…18 The usual methods applicable for orange juice sample preparation involve the organic matter destruction. Dry ashing in a muffle furnace, 3,5,7,8,12,13 wet digestion procedure using a hot plate, 4,10,14 or microwave-assisted oven 9,12,13,[15][16][17] have been used as orange juice sample preparation for elemental analysis. For some elements determination, such as Al, a simple filtration has been proposed.…”

Section: Etaas Icp-oes and Icp-ms Whereas Simultaneous Icp-mentioning

confidence: 99%

On-line digestion in a focused microwave-assisted oven for elements determination in orange juice by inductively coupled plasma optical emission spectrometry

Fili¹,

Oliveira²,

Oliveira³

2003

J. Braz. Chem. Soc.

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Um sistema de fluxo acoplado a um forno de microondas focalizadas foi utilizado para digestão em linha de amostras de suco de laranja para a determinação de Ca, Cu, Fe, K, Mg, Mn, Na, P e Zn por espectrometria de emissão ótica com plasma indutivamente acoplado. Uma bobina de reação de PTFE (4,0 m de comprimento e 1,6 mm de diâmetro interno) foi posicionada no interior da cavidade de vidro do forno de microondas focalizadas. Alíquotas de 500 µL de amostra e de 1000 µL de reagente (80% v/v HNO 3 ) foram misturadas em uma confluência e conduzidas para a bobina de reação utilizando ar como carregador. O desvio padrão relativo para cinco determinações da mesma amostra usando o método proposto foi inferior a 5,0%. Boas recuperações variando de 91 a 111% foram obtidas para adições de quantidades conhecidas dos elementos de interesse. Os resultados obtidos, utilizando o sistema de digestão proposto, foram concordantes a um nível de confiança de 95% com aqueles obtidos por digestão total. Com este método de digestão foi possível processar 12 amostras h -1 , minimizando contaminações, consumo de amostra e reagentes e gerando pequena quantidade de resíduos.A flow injection system coupled to focused microwave-assisted oven was used for on-line orange juice sample digestion for determination of Ca, Cu, Fe, K, Mg, Mn, Na, P, and Zn by inductively coupled plasma optical emission spectrometry. The reactor coil was a PTFE tube (4.0 m long and 1.6 mm i.d.) positioned into the commercial glass tube of the focused microwave oven. Aliquots of 500 µL of sample and 1000 µL of reagent (80% v/v HNO 3 ) were mixed in a confluence and carried out to the reactor coil by air carrier. The relative standard deviation for five replicates of sample was lower than 5.0%. Good recoveries varying from 91 to 111% were obtained for added concentrations of the interest elements. The results obtained using the proposed digestion system are in agreement with those obtained for total digestion at the 95% confidence level. With this on-line digestion system was possible to carry out 12 samples h -1 , minimizing contamination, saving consumption of samples and reagent and low residue generation.

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“…Many sophisticated analytical techniques, hydride generation-atomic absorption spectrometry (HG-AAS) [16,17], inductively coupled plasma-mass spectrometry (ICP-MS) [18,19], hydride generation-atomic fluorescence spectrometry (HG-AFS) [20][21][22], high performance liquid chromatographyatomic fluorescence spectrometry (HPLC-AFS) [23] and differential pulse anodic stripping voltammetry [24,25] are used for the determination of antimony in environmental and biological samples. These techniques are quite expensive and require costly maintenance and skilled hands for operation.…”

Section: Introductionmentioning

confidence: 99%

On-site spectrophotometric determination of antimony in water, soil and dust samples of Central India

Shrivas

Agrawal

Harmukh³

2008

Journal of Hazardous Materials

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Determination of antimony species with fluoride as modifier and flow injection hydride generation inductively-coupled plasma emission spectrometry

Cited by 32 publications

References 17 publications

Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani

Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani

On-line digestion in a focused microwave-assisted oven for elements determination in orange juice by inductively coupled plasma optical emission spectrometry

On-site spectrophotometric determination of antimony in water, soil and dust samples of Central India

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