Determination of Antioxidant Activity of Some Drugs Using High-pressure Liquid Chromatography

Karunakar, N; Prabhakar, M. C.; Krishna, D. R.

doi:10.1055/s-0031-1297105

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…The HPLC method is sensitive and can be used as a quality control tool for the rapid determination of free radical scavenging activity of variety of products including plant extracts, foods, drugs and polyherbal formulations. The applicability of HPLC method was extended to determine the antioxidant activity of crude plant extracts and drugs (Dapkevicius et al 1999;Karunakar et al 2003). The HPLC method developed was specific for DPPH with an acceptable reproducibility and short run time allowing for rapid determination of radical scavenging activity of several samples (Chandrasekar et al 2006).…”

Section: Developments In Dpph Methodsmentioning

confidence: 99%

Genesis and development of DPPH method of antioxidant assay

2011

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α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

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Section: Developments In Dpph Methodsmentioning

confidence: 99%

Genesis and development of DPPH method of antioxidant assay

2011

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“…Also, the DPPH spectrophotometric method of evaluation may not be of much use to judge the antioxidant activity, as it is not capable to indicate the antioxidant activity of drugs such as nimesulide, dapsone and acetylsalicylic acid etc. [20][21][22]. Few workers developed DPPH-HPLC method and compared the antioxidant activities of known standard antioxidants and commercial beverages with that of spectrophotometric method.…”

Section: Rs+rs=rs Sr (3)mentioning

confidence: 99%

Determination of DPPH Free Radical Scavenging Activity by RP-HPLC, Rapid Sensitive Method for the Screening of Berry Fruit Juice Freeze Dried Extract

Kumara¹,

Sunil²,

B³

2018

Nat Prod Chem Res

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USP official UV-VIS spectrophotometric method for the evaluation of antioxidant activity by scavenging the DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical was not suitable to determine the antioxidant activity of Berry fruit juice freeze dried extract due to interferences from the matrix. Interference was also observed from the color pigments of the Berry fruit juice freeze dried extract. This has leads to the development of a specific analytical method for evaluation of the DPPH free radical scavenging activity of Berry fruit juice freeze dried extract. RP-HPLC method was developed on a Phenomenex Luna-C18 Column (250 mm × 4 mm, 5 µM) with mobile phase methanol and water mixed online in the ratio of 80:20 (v/v), pumped at a flow rate of 1 mL/min. DPPH peaks were quantified at wavelength 517 nm. Method was standardized using the anti-oxidant ascorbic acid. The 50% free radical scavenging activity (IC 50 ) determined by the novel HPLC method was correlating with the results obtained by USP UV-VIS spectrophotometric method. While the USP UV-VIS spectrophotometric method failed to estimate the free radical scavenging activity of Berry fruit juice freeze dried extract, whereas the developed HPLC method was specific, sensitive, robust, accurate and precise and rapid in the determination.The objective of the present study is to develop, standardize and compare the DPPH-HPLC method with that of spectrophotometric method using known antioxidants, and to evaluate its application to determine the antioxidant activity of selected berry fruit juice freeze dry powder as a means of rapid screening methodology.

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“…DPPH-HPLC assay developed by Yamaguchi et al (1998) was later extended to determine the crude plant extracts and drugs antioxidant activity (Karunakar et al 2003;Krishnaiah et al 2004).…”

Section: Determination Of Antioxidant Activitymentioning

confidence: 99%

Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae

et al. 2009

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Antioxidant activities of both cells and extracellular substances were evaluated in 12 soil-isolated strains of microalgae according to FRAP and DPPH-HPLC assays. Their total phenolic contents were also determined by Folin-Ciocalteu method. Extractions were performed with hexane, ethyl acetate, and water. The results of FRAP assay showed that algal cells contained considerable amounts of antioxidants from 0.56 ± 0.06 to 31.06 ± 4.00 µmol Trolox g −1 for Microchaete tenera hexane extract and Chlorella vulgaris water extract, respectively. In water fractions of extracellular substances, the antioxidants were from 1.30±0.15 µmol Trolox g −1 for Fischerella musicola to 73.20±0.16 µmol Trolox g −1 for Fischerella ambigua. Also, DPPH-HPLC assay represented high antioxidant potential of water fractions. The measured radicalscavenging activities of the studied microalgae were at least 0.15±0.02 in Nostoc ellipsosporum cell mass to a maximum of 109.02±8.25 in C. vulgaris extracellular substance. The amount of total phenolic contents varied in different strains of microalgae and ranged from zero in hexane extract to 19.15±0.04 mg GAE g −1 in C. vulgaris extracellular water fraction. Significant correlation coefficients between two measured parameters indicated that phenolic compounds were a major contributor to the microalgal antioxidant capacities.

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Determination of Antioxidant Activity of Some Drugs Using High-pressure Liquid Chromatography

Cited by 11 publications

References 7 publications

Genesis and development of DPPH method of antioxidant assay

Genesis and development of DPPH method of antioxidant assay

Determination of DPPH Free Radical Scavenging Activity by RP-HPLC, Rapid Sensitive Method for the Screening of Berry Fruit Juice Freeze Dried Extract

Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae

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