Determination of Antioxidant Capacity and Free Radical Scavenging Activity of Milk from Native Cows (Bos Indicus ), Exotic Cows (Bos Taurus ), and Riverine Buffaloes (Bubalus Bubalis ) Across Different Lactation Stages

Mukesh, Manishi; Mann, Sabine; Uk, Shandilya; Kumar, Pradeep; Mohanty, Ayesha

doi:10.19070/2379-1578-1600013

Cited by 8 publications

(4 citation statements)

References 21 publications

(23 reference statements)

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“…It should be emphasized that fat-soluble vitamins, primarily E and A, are the main antioxidants derived from the fat fraction protecting cells against reactive oxygen species [ 36 , 39 ]. Their activity consists of scavenging organic free radicals and inhibiting lipid peroxidation [ 73 ]. Similarly, Kuczyńska et al [ 65 ] reported an increase in the content of vitamins E and A in milk fat after supplementation with a mixture of oregano, cumin, and rosemary.…”

Section: Resultsmentioning

confidence: 99%

Effects of Supplementation with an Herbal Mixture on the Antioxidant Capacity of Milk

Stobiecka

Król

Brodziak

et al. 2023

Animals

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The aim of this study was to assess the effect of the addition of a standardized herbal mixture to the feed ration for Holstein-Friesian cows on the antioxidant capacity of milk. The study was carried out on a farm specialized in breeding dairy cattle. The exact study involved 30 cows in lactation III, which were in the first phase of lactation at the beginning of the experiment (15 cows—control group; 15 cows—experimental group). The nutrition supplied to the cows was based on the TMR (total mixed ration) system, with roughage and concentrate fodder used as the basis of the feed ration. The addition of a standardized blend of dried herbs, i.e., oregano (Origanum vulgare), thyme (Thymus vulgaris), purple coneflower (Echinacea purpurea), and cinnamon bark (Cinnamomum zeylanicum), was the experimental factor. Powdered herbs were administered as a component of the concentrate fodder at the dose of 3% DM ration/day/head. Milk samples were collected four times during the experiment (term 0 after the colostrum period and then after lactation weeks 2, 4, and 6). The following parameters were determined in the milk: the basic chemical composition, i.e., the content of total protein, fat, lactose, and casein; somatic cell count; content of selected whey proteins (α-lactalbumin, β-lactoglobulin, lactoferrin, BSA); and fat-soluble vitamins (A, D3, E). Additionally, the milk antioxidant capacity (ABTS, FRAP, DPPH) was determined and the degree of antioxidant protection (DAP) was calculated. It was shown that the milk from cows receiving the herbal blend-supplemented fodder had a higher content of casein, compared to the control group. The herbal supplementation contributed to a significant increase in the content of bioactive compounds, i.e., selected whey proteins (β-lactoglobulin, lactoferrin) and lipophilic vitamins (A, E). The milk was also characterized by significantly higher antioxidant potential (regardless of the measurement method) and a higher degree of antioxidant protection (DAP).

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Section: Resultsmentioning

confidence: 99%

Effects of Supplementation with an Herbal Mixture on the Antioxidant Capacity of Milk

Stobiecka

Król

Brodziak

et al. 2023

Animals

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“…In contrast, Mann et al . [ 36 ] found that during the early and peak stages of lactation, TAC levels were lower in milk from Sahiwal cows (indigenous Indian cattle) than in milk from Karan Fries cows (hybrids). Our results suggest that the immune cells of Brahman × Thai native crossbreed cattle have better antioxidant capacity than those of Brahman cattle, indicating that crossbreed cattle are more resistant to stress responses and oxidative stress than purebred breeds.…”

Section: Discussionmentioning

confidence: 99%

Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman x Thai native crossbreed cattle

Srinontong,

Aengwanich,

Somphon

et al. 2024

Vet World

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Background and Aim: Lipopolysaccharide (LPS) is a robust endotoxin known to activate the immune system in cattle. The objective of this study was to investigate the effect of LPS on the morphology, cell viability, malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC) of peripheral blood mononuclear cells (PBMCs) in Brahman and Brahman x Thai native crossbreed cattle. Materials and Methods: PBMCs were isolated from Brahman and Brahman x Thai native crossbreed cattle and treated with 0, 0.1, 1, and 10 μg/mL Escherichia coli LPS, respectively. Morphological changes in PBMCs were assessed at 24 and 48 h. In addition, we measured PBMC cell viability, MDA, NO, and TAC. Results: LPS stimulation caused cell deformation and partial PBMC area enlargement, but there were no differences between Brahman and Brahman x Thai native crossbreed cattle. Stimulation at all levels did not affect the viability of PBMCs (p > 0.05). MDA and NO levels were significantly higher in Brahman cattle than in Brahman Thai native crossbred cattle (p < 0.05). TAC was significantly higher in Brahman x Thai native crossbred cattle than in Brahman cattle (p < 0.05). Conclusion: Immune cells of crossbreed cattle have a higher activation response to LPS than those of purebred cattle, and native crossbreed beef cattle have a higher antioxidant capacity than purebred beef cattle. This result may explain why hybrid cattle of indigenous breeds are more resistant to disease than purebred cattle. Keywords: Brahman cattle, lipopolysaccharide, oxidative stress, peripheral blood mononuclear cells, Thai native crossbreed cattle.

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“…Vitamins (soluble in either milk fat or milk serum) and minerals play an essential role as antioxidant factors. The antioxidant capacity of vitamins E (α-tocopherol), A, and C (ascorbic acid) as well as carotenoids is due to their ability to scavenge free radicals (mainly oxygen, hydroxyl, and peroxyl radicals), inhibit lipid peroxidation, and protect DNA from damage [17,18]. Supplementation of milk with ascorbic acid in light-exposed milk enhanced the antioxidant capacity of milk and inhibited the degradation of riboflavin [19].…”

Section: Milk Component With Antioxidant Activities: An Overviewmentioning

confidence: 99%

Recent Development in Antioxidant of Milk and Its Products

Elshaghabee¹,

El-Maksoud²,

Gouveia³

2023

Biochemistry

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Free radicals are produced in humans through natural metabolism or the external environment, such as diet. These free radicals are neutralized by the antioxidant system, whereas enzymes, for example, catalase, superoxide dismutase, and glutathione peroxidase, play an important role in preventing excessive free radicals. Food antioxidants give a good hand in enhancing the human antioxidant system; high consumption of a diet rich in natural antioxidants protects against the risk of diseases such as cardiovascular, cancer, diabetes, and obesity. Milk and its products are popular for a wide range of consumers. Milk contains casein, whey protein, lactoferrin, milk lipid and phospholipids, vitamins, and microelements, for example, selenium (Se), which have antioxidant properties. Furthermore, probiotication of milk either sweet or fermented could enhance the antioxidant capacity of milk. This chapter focuses on presenting recent review data on milk components with antioxidant activity and their health benefits, probiotics as antioxidant agents, and methods for enhancing the antioxidant capacity of dairy products. The key aim of this chapter is to focus on major strategies for enhancing the antioxidant capacity of milk and its products.

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Determination of Antioxidant Capacity and Free Radical Scavenging Activity of Milk from Native Cows (Bos Indicus ), Exotic Cows (Bos Taurus ), and Riverine Buffaloes (Bubalus Bubalis ) Across Different Lactation Stages

Cited by 8 publications

References 21 publications

Effects of Supplementation with an Herbal Mixture on the Antioxidant Capacity of Milk

Effects of Supplementation with an Herbal Mixture on the Antioxidant Capacity of Milk

Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman x Thai native crossbreed cattle

Recent Development in Antioxidant of Milk and Its Products

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