Determination of Antioxidants by DPPH Scavenging Activity of Ashitaba Herb (Angelica keiskei) Methanol Extract

Abstract

Ashitaba (Angelica keiskei) is one of the plants cultivated in the Sembalun region, Lombok, NTB, which has the potential to be developed as a source of antioxidants. This study aims to determine the antioxidant activity of the methanol extract of ashitaba herb by measuring the inhibition against DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals using a UV-Vis spectrophotometer. Ashitaba herb samples were collected in Jorong Hamlet, Sembalun Bumbung Village, Sembalun District, East Lombok Regency at coordinates 8°23'5.273" LAT - l16°32'12.016" EAST. The sample was then processed into simplisia with a simplisia yield of 14.75%. The simplisia was dechlorinated with n-hexane solvent and then extracted with 80% methanol solvent using the sonic method, giving a methanol extract of ashitaba herb yielding 20.30%. DPPH radical inhibitory activity was measured at a maximum wavelength of 516 nm and an operating time (OT) of 30 minutes. The methanol extract of ashitaba herb has an IC50 value of 129.40 ± 7.36 ppm, which shows moderate antioxidant activity, with a standard compound comparison of ascorbic acid with an IC50 value of 2.37 ± 0.06 ppm, which is classified as very potent.