Determination of antiplatelet antibodies on the surface of platelets from patients with various forms of immune thrombocytopenia by direct radioimmunoassay

Kuznetsov, A. I.; Idel'son, L I; Мазуров, А. В.

doi:10.1007/bf00840201

Cited by 2 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Control group comprised healthy donors with a platelet count above 200x109 cells/liter. PA-lgG was assayed by radioimmunoassay [4], and the content of serum platelet-reactive antibodies was measured by enzyme-linked immunosorbent assay [3].…”

Section: Methodsmentioning

confidence: 99%

Assay of antigens of antiplatelet antibodies in patients with various thrombocytopenias

et al. 1998

Self Cite

View full text Add to dashboard Cite

Sandwich enzyme-linked immunosorbent assay was developed for evaluation of target antigens reacting with antiplatelet auto-and alloantibodies. The method based on specific immobilization of platelet antigens via monoclonal antibodies was applied to identify antigens of platelet-associated and serum autoantibodies in patients with different thrombocytopenias and to determine platelet HPA-1 alloantigens.

show abstract

Section: Methodsmentioning

confidence: 99%

Assay of antigens of antiplatelet antibodies in patients with various thrombocytopenias

et al. 1998

Self Cite

View full text Add to dashboard Cite

show abstract

“…Plermm Publishing Corporation Direct radioimmunoassay was used to detect antibodies associated with the platelet surface [3,6]. The number of plat 9 immunoglobulins (PAIgG) was assessed from the binding of ~25I-labeled antibodies to human IgG (125I-anti-IgGh) with patient platelets washed free of plasma and expressed in ng/106 platelets.…”

Section: Methodsmentioning

confidence: 99%

Autoantibodies to platelets in children with thrombocytopenia

et al. 1995

Self Cite

View full text Add to dashboard Cite

Antiplatelet antibodies were titered in children and adults with idiopathic thrombocytopenic purpura. The levels of platelet-associated IgG were elevated in 100% of the examined children and 86% of the adults, their mean titers being at least 10 times higher than in the control and differing little within the groups. Serum antibodies reacting with normal donor platelets were detected in 43% of adults and in only 18% of children. Improvement of the clinical picture and increase of the platelet count in these patients according to different protocols always correlated with a drop in the level of antiplatelet antibodies. The resuits point to the immune nature of idiopathic thrombocytopenic purpura in children.

show abstract

Determination of antiplatelet antibodies on the surface of platelets from patients with various forms of immune thrombocytopenia by direct radioimmunoassay

Cited by 2 publications

References 12 publications

Assay of antigens of antiplatelet antibodies in patients with various thrombocytopenias

Assay of antigens of antiplatelet antibodies in patients with various thrombocytopenias

Autoantibodies to platelets in children with thrombocytopenia

Contact Info

Product

Resources

About