Determination of artemisinin and artemisinic acid by capillary and packed supercritical fluid chromatography

Köhler, Marcel; Haerdi, W.; Christen, Philippe; Veuthey, Jean‐Luc

doi:10.1002/jhrc.1240200203

Cited by 18 publications

(6 citation statements)

References 13 publications

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“…However, they were not fast and sensitive enough to quantitate the large number of samples in biological matrices, necessary to accomplish in a pharmacokinetic study in small animals [19,20]. An improvement in chromatographic performance has been achieved by the introduction of ultra-performance liquid chromatography (UPLC).…”

Section: Spiked Concentration (Ng/ml)mentioning

confidence: 98%

Determination of antimalarial compound, ARB-89 (7β-hydroxy-artemisinin carbamate) in rat serum by UPLC/MS/MS and its application in pharmacokinetics

Pabbisetty

Illendula

Muraleedharan

et al. 2012

Journal of Chromatography B

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Section: Spiked Concentration (Ng/ml)mentioning

confidence: 98%

Determination of antimalarial compound, ARB-89 (7β-hydroxy-artemisinin carbamate) in rat serum by UPLC/MS/MS and its application in pharmacokinetics

Pabbisetty

Illendula

Muraleedharan

et al. 2012

Journal of Chromatography B

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“…Previously, we have developed a simultaneous isolation of artemisinin and its precursors from A. annua by preparative reversed-phase HPLC, but it requires expensive instrument under laboratory settings. 16) Others reported the extraction of artemisinin and artemisinic acid from A. annua using supercritical carbon dioxide, 17) but they did not explore the technology to extract DHAA.…”

Section: )mentioning

confidence: 99%

Isolation of Dihydroartemisinic Acid from <i>Artemisia annua</i> L. By-Product by Combining Ultrasound-Assisted Extraction with Response Surface Methodology

Liu

Ferreira

Liu

et al. 2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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Malaria is the most devastating parasitic disease worldwide. Artemisinin is the only drug that can cure malaria that is resistant to quinine-derived drugs. After the commercial extraction of artemisinin from Artemisia annua, the recovery of dihydroartemisinic acid (DHAA) from artemisinin extraction by-product has the potential to increase artemisinin commercial yield. Here we describe the development and optimization of an ultrasound-assisted alkaline procedure for the extraction of DHAA from artemisinin production waste using response surface methodology. Our results using this methodology established that NaOH at 0.36%, extraction time of 67.96 min, liquid-solid ratio of 5.89, and ultrasonic power of 83.9 W were the optimal conditions to extract DHAA from artemisinin production waste. Under these optimal conditions, we achieved a DHAA yield of 2.7%. Finally, we conducted a validation experiment, and the results confirmed the prediction generated by the regression model developed in this study. This work provides a novel way to increase the production of artemisinin per cultivated area and to reduce artemisinin production costs by recycling its commercial waste to obtain DHAA, an immediate precursor of artemisinin. The use of this technology may reduce the costs of artemisinin-based antimalarial medicines.Key words dihydroartemisinic acid; ultrasound-assisted extraction; response surface; Artemisia annua L.; alkaline extraction; acid precipitation Malaria is one of the world's most important parasitic diseases, affects approximately 300-500 million people worldwide, and causes more than one million deaths per year (WHO: World Malaria Report 2015, http://www.who.int/ malaria/publications/world-malaria-report-2015/report/en/). 1)Artemisia annua, L. is currently the only commercial source of artemisinin, the raw material for the production of artemisinin combination therapies (ACTs). ACTs are the frontline and life-saving medicine to treat malaria where Plasmodium falciparum is endemic and resistant to quinine-derived medicines. ACTs cost between US$ 1.0 and 3.50 per treatment and can be required many times a year by people living in malaria-endemic areas. However, at the current cost ACTs are unaffordable for people living in economically-stricken countries, and who need it the most.2) Thus, it is of paramount importance and urgency to reduce the production costs of artemisinin-derived antimalarial medicines. One way to achieve this goal is by increasing artemisinin yield per cultivated area and by improving the extraction efficiency of artemisinin and its related compounds from leaves of A. annua. Although the production of one of the artemisinin precursors (artemisinic acid) in genetically-engineered yeast was developed, no economically feasible artemisinin product is yet available from this technology, and its predicted cost is higher than current market prices for plant-based artemisinin. The production of artemisinin in planta surpasses what can be achieved by microorganisms engineered to produce the precurs...

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“…For the analysis of diterpenes in rosemary extracts see chapter "Preparative/semipreparative applications". The SFC separation of two sesquiterpene lactones in Artemisia annua, artemisinin and artemisinic acid, was described by Kohler et al [86]. They compared two different approaches, either using a packed aminopropyl silica column (Nucleosil NH 2 ) together with ELSD, or a DB-WAX capillary column and an FID.…”

Section: Analyticalmentioning

confidence: 99%

Supercritical Fluid Chromatography – Theoretical Background and Applications on Natural Products

Hartmann

Ganzera

2015

Planta Med

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The use of supercritical fluid chromatography for natural product analysis as well as underlying theoretical mechanisms and instrumental requirements are summarized in this review. A short introduction focusing on the historical development of this interesting separation technique is followed by remarks on the current instrumental design, also describing possible detection modes and useable stationary phases. The overview on relevant applications is grouped based on their basic intention, may it be (semi)preparative or purely analytical. They indicate that supercritical fluid chromatography is still primarily considered for the analysis of nonpolar analytes like carotenoids, fatty acids, or terpenes. The low polarity of supercritical carbon dioxide, which is used with modifiers almost exclusively as a mobile phase today, combined with high efficiency and fast separations might explain the popularity of supercritical fluid chromatography for the analysis of these compounds. Yet, it has been shown that more polar natural products (e.g., xanthones, flavonoids, alkaloids) are separable too, with the same (if not superior) selectivity and reproducibility than established approaches like HPLC or GC.

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Determination of artemisinin and artemisinic acid by capillary and packed supercritical fluid chromatography

Cited by 18 publications

References 13 publications

Determination of antimalarial compound, ARB-89 (7β-hydroxy-artemisinin carbamate) in rat serum by UPLC/MS/MS and its application in pharmacokinetics

Determination of antimalarial compound, ARB-89 (7β-hydroxy-artemisinin carbamate) in rat serum by UPLC/MS/MS and its application in pharmacokinetics

Isolation of Dihydroartemisinic Acid from <i>Artemisia annua</i> L. By-Product by Combining Ultrasound-Assisted Extraction with Response Surface Methodology

Supercritical Fluid Chromatography – Theoretical Background and Applications on Natural Products

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