Determination of ATP and its metabolites in dietary energy supplements by capillary electrophoresis

Büyüktuncel, Ebru

doi:10.1590/s2175-97902022e201045

Cited by 1 publication

(2 citation statements)

References 17 publications

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“…Moreover, sample purification is a significant limitation of that method [36]. In the case of dietary energy supplements containing only one nucleotide and no other additives, sample preparation is very simple, because the tablet can be ground, dissolved in water, and filtered [37]. Admittedly, that method is simpler than the one we developed, but it is limited in its applicability to supplements containing only one group of compounds-nucleotides [37].…”

Section: Application Of the Developed Extraction Methods To Dietary S...mentioning

confidence: 99%

“…In the case of dietary energy supplements containing only one nucleotide and no other additives, sample preparation is very simple, because the tablet can be ground, dissolved in water, and filtered [37]. Admittedly, that method is simpler than the one we developed, but it is limited in its applicability to supplements containing only one group of compounds-nucleotides [37]. There are very few such supplements today, because the composition of most of them is more complex and contains compounds belonging to different groups (e.g., nucleotides, vitamins, yeast, organic acids).…”

Section: Application Of the Developed Extraction Methods To Dietary S...mentioning

confidence: 99%

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Extraction of Nucleotides from Dietary Supplements by Newly Synthesized Adsorbents

Studzińska,

Bocian,

Stypczyńska

et al. 2023

Foods

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The aim of the study was the synthesis and application of novel adsorbents for the extraction of nucleotides from dietary supplements. Three different adsorbents modified with a silane containing two amine groups and various dicarboxylic acids were synthesized and characterized using various instrumental techniques. Next, different solvents were tested for their adsorption and desorption of five nucleotides. The results showed that the efficiency of both processes depends on the conditions used and the type of dicarboxylic acid bound to the surface of the adsorbent. The best results were obtained for succinic acid. The most effective adsorption occurred for water acidified with acetic acid to pH 4.5, while the highest recoveries (85–102%) with high reproducibility were obtained for 10 mM ammonium acetate at pH 9. The nucleotide extraction was performed simply by changing the charge at the adsorbent surface, providing the possibility of electrostatic attraction and repulsion between the adsorbent and nucleotides. Moreover, the sorption capacity of the obtained materials was also determined, which was essential for their use in extracting nucleotides from real samples by dispersive extrusion to the solid phase. The new adsorbents and the developed extraction method were successfully applied to isolate nucleotides from two different dietary supplements with different compositions (one of them with yeast strains). The method is simple and reproducible; no organic solvents or high-concentration inorganic salts are used (it is environmentally friendly). The entire process is performed in one centrifuge tube and is cheaper compared with methods used so far.

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Section: Application Of the Developed Extraction Methods To Dietary S...mentioning

confidence: 99%

Section: Application Of the Developed Extraction Methods To Dietary S...mentioning

confidence: 99%

Extraction of Nucleotides from Dietary Supplements by Newly Synthesized Adsorbents

Studzińska,

Bocian,

Stypczyńska

et al. 2023

Foods

View full text Add to dashboard Cite

show abstract

Determination of ATP and its metabolites in dietary energy supplements by capillary electrophoresis

Cited by 1 publication

References 17 publications

Extraction of Nucleotides from Dietary Supplements by Newly Synthesized Adsorbents

Extraction of Nucleotides from Dietary Supplements by Newly Synthesized Adsorbents

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