Determination of Bactericidal Potential of Green Based Silver and Zinc Nanoparticles against Bacterial Canker of Tomato

Abstract

Bacterial canker of tomato is the most damaging one and is responsible for causing 70% yield losses each year. Green synthesis of nanoparticles has ushered in a new research field known as green nanotechnology and has emerged as vital tool for the management of plant diseases. In comparison to other conventional techniques, green synthesis of various nanoparticles was found to be eco-friendly, low-cost and profitable resulting in more stable synthesized materials. In present research, zinc and silver nanoparticles were synthesized and their antibacterial potential was evaluated alone and in combination against Clavibacter michiganensis subsp. michiganensis under lab and field conditions. In lab experiment, combination of Zinc and Silver NPs proved to be the most effective showing maximum inhibition zone (27.449 mm) followed by Zinc (19.50 mm), and Silver (16.21 mm). Similarly, in the field experiment combination of Ag+Zn NPs proved effective with minimum disease incidence (6.10%) followed by Zinc (10.15%) and Silver NPs (22.40%) as compared to the control.

Green inspired synthesis of zinc oxide nanoparticles using Silybum marianum (milk thistle) extract and evaluation of their potential pesticidal and phytopathogens activities

“…Tribolium castaneum and Sitophilus oryzae are destructive wheat, rice, and maize pests worldwide. Clavibacter michiganensis and Pseudomonas syringes are gram-positive and gram-negative phytopathogenic bacterial strains responsible for canker ( Atiq et al, 2022 ) and wilting in tomatoes, ring rot in potatoes, and bacterial apical necrosis (BAN) in mango trees ( Miller et al, 2019 ). Similarly, Fusarium oxysporum and Aspergillus niger also cause black mold disease in vegetables and fruits such as apricots, onions, grapes, and peanuts ( Tolossa & Shibeshi, 2022 ).…”

Evaluation of virulence among Pakistani isolates of Ralstonia solanacearum inducing bacterial wilt in chilies across different agro-ecological zones

“…The isolates of the bacterium were further characterized on the basis of morphology, i.e., by their growth patterns (mucoid and non-mucoid growth) and biochemical tests (Atiq et al 2022;Khurshid et al 2022) viz. gram reaction, catalase activity, levan production (Schaad 1988;Rahoo et al 2022), KOH loop test (Suslow et al 1982), oxidase activity (Kovacs 1956), lipase activity, pigment production (King et al 1954), arginine dihydrolase reaction (Thornley 1960), gas production (Van den Mooter et al 1987), oxidation, and fermentation activity (Hayward 1964).…”

Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan

“…The isolates were characterized morphologically by their growth patterns (mucoid and nonmucoid growth) and biochemically by employing various biochemical tests (Atiq et al 2022;Khurshid et al 2022), viz. gram reaction, catalase activity, levan production (Rahoo et al 2022;Schaad 1988), KOH loop test (Suslow et al 1982), oxidase activity (Kovacs 1956), lipase activity, pigment production (King et al 1954), arginine dihydrolase reaction (Thornley 1960), gas production (van den Mooter 1987), oxidation and fermentation activity (Hayward 1964).…”