Determination of binding constants of Ca2+, Na+, and Cl− ions to liposomal membranes of dipalmitoylphosphatidylcholine at gel phase by particle electrophoresis

Satoh, Koichi

doi:10.1016/0005-2736(95)00154-u

Cited by 53 publications

(62 citation statements)

References 38 publications

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“…For this purpose available literature experimental results and molecular modeling (CHARMM36 force field model) were used (Satoh 1995, Liu et al 2001Warshawiak et al 2011;Clarke 2001) and the constructed scheme of the potential profile across DOPC bilayer is drawn in Fig. 6.…”

Section: Profile Of Electric Potential Across the Liposome Bilayermentioning

confidence: 99%

“…6 A scheme of the electrical potential profile across the DOPC bilayer based on the data from ref. (Satoh 1995;Liu et al 2001;Warshawiak et al 2011;Clarke 2001) 5 Surface potential and surface charge of the liposome Finally, also it seemed interesting to calculate the surface potential W o and then the surface charge of the liposomes using the measured zeta potential values. The surface potential was calculated from Eq.…”

Section: Profile Of Electric Potential Across the Liposome Bilayermentioning

confidence: 99%

“…The slip plane location was assumed to be at x = 0.24 nm from the liposome surface independently of the ionic concentration as postulated by Satoh (1995). In Table 1 are listed, the Debye-Hückel parameter j and ja product, the zeta potential f calculated from Henry's equation, and the surface potential W o calculated from Eq.…”

Section: Profile Of Electric Potential Across the Liposome Bilayermentioning

confidence: 99%

“…Remarkable value of zeta potential, say[20 mV, should stabilize the liposomes and protect against their aggregation. Therefore, the zeta potential changes might be a valuable source of information shedding an additional light on liposome properties, including the hydrolysis reaction (Yamauchi et al 1988;Satoh 1995;Vaccaro et al 1997;Liu et al 2001;Ikonen et al 2010). However, there are not many published papers on the zeta potential changes of liposomes occurring during the enzymatic hydrolysis reactions.…”

Section: Introductionmentioning

confidence: 99%

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Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

2016

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Zeta potential of liposomes is often measured in studies of their properties and/or applications. However, there are not many papers published in which zeta potential was determined during enzymatic reaction of a lipid. Therefore in this paper size, polydispersity index, and zeta potentials of 1,2-dipalmitoylsn-glycero-3-phosphatidylcholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) liposomes were investigated in 1 mM NaCl (pH 6.2) and phosphate buffer (pH 8.1) during 60 min of phospholipase C action at 20 and 37°C. The hydrocarbon chains saturation differ these two phospholipids what appears in some differences in the zeta potential changes during the hydrolysis reaction run. The polydispersity index of the liposomes indicates that they are relatively stable and monodisperse, except for DPPC in phosphate buffer where up to 30 % of the initial amount forms larger size moieties, possibly aggregates of the liposomes, enzyme and the hydrolysis products. However, in this buffer zeta potential of the liposomes practically does not change during PLC action. Also the changes of zeta potential of DOPC liposomes are minor, although their negative values are much smaller than those of DPPC at both temperatures. These small changes of the potential may be due to compression of the diffuse double layer by present phosphate buffer. However, distinct changes of the zeta potentials in the presence of PLC take place in NaCl solution. The observed changes can be explained by reorientation of the phospholipid polar heads and their different density on DPPC and DOPC surface of the liposomes. Although it appeared that the zeta potential is not a very sensitive parameter for tracking the hydrolysis reaction in phosphate buffer, generally zeta potential enables the characterization of such reactions through determination of electrokinetic properties of liposomes as well as the polydispersity and size distribution of the liposomes do.

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Section: Profile Of Electric Potential Across the Liposome Bilayermentioning

confidence: 99%

Section: Profile Of Electric Potential Across the Liposome Bilayermentioning

confidence: 99%

Section: Profile Of Electric Potential Across the Liposome Bilayermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

2016

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“…Further increases in temperature cause a rise in the quasilamellar segments within the membrane in the intermediate periodic phase P β . In this phase, the surface of the membrane is usually rippled, which occurs at T p temperature [23]. After the intermediate phase, above T p temperature, the hydrocarbon chains start to melt and form trans-gauche isomers.…”

Section: Model Membrane Phase Transitionsmentioning

confidence: 99%

Dynamics of Model Membranes by NMR

Timoszyk¹

2017

Spectroscopic Analyses - Developments and Applications

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Amphiphilic molecules can create various aggregates in water. Concern about exploring such structures has been unabated for several decades due to the wide range of possible applications of lipid aggregates, from food technology to the pharmaceutical industry. The form of self-assembled structures depends on many factors, such as the type of amphiphilic molecule, the concentration, the level of hydration, the temperature, and the pH. Liposomes and micelles are the most widely known types of closed structures. Liposomes are more often used in the fields of medicine and pharmacy because they consist of nontoxic compounds and their composition and size can be controlled. Nuclear magnetic resonance (NMR) is one of the methods, which is most commonly used to study liposome properties. It can be used to observe changes in the structure, dynamics, and phase transition of lipid membranes. The membrane properties are changed under the influence of external factors, such as temperature, pH, and the presence of ions or drugs. The chapter aims to introduce and discuss the possibilities of the most useful NMR methods, 31 P and 1 H, to study the liposome properties. It also aims to show how various changes in the structure or dynamics of lipid molecules are visible in the NMR spectra.

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Solvation of Calcium–Phosphate Headgroup Complexes at the DPPC/Aqueous Interface

2015

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The effects of sodium (Na(+) ) and calcium (Ca(2+) ) cations on model zwitterionic dipalmitoylphosphatidylcholine (DPPC) monolayers spread on metal chloride salt solutions are investigated by means of vibrational sum frequency generation (VSFG) and heterodyne-detected (HD)-VSFG spectroscopy. VSFG and HD-VSFG spectra in the OH stretching region reveal cation-specific effects on the interfacial water's H-bonding network, knowledge of which has been limited to date. It is found that low-concentrated Ca(2+) more strongly perturbs interfacial water organization relative to highly concentrated Na(+) . At higher Ca(2+) concentrations, the water H-bonding network at the DPPC/CaCl2 interface reorganizes and the resulting spectrum closely follows that of the bare air/CaCl2 interface up to ∼3400 cm(-1) . Most interesting is the appearance of a negative band at ∼3450 cm(-1) in the DPPC/CaCl2 Im χs ((2)) spectra, likely arising from an asymmetric solvation of Ca(2+) -phosphate headgroup complexes. This gives rise to an electric field that orients the net OH transition moments of a subset of OH dipoles toward the bulk solution.

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Determination of binding constants of Ca2+, Na+, and Cl− ions to liposomal membranes of dipalmitoylphosphatidylcholine at gel phase by particle electrophoresis

Cited by 53 publications

References 38 publications

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

Dynamics of Model Membranes by NMR

Solvation of Calcium–Phosphate Headgroup Complexes at the DPPC/Aqueous Interface

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